Amino terminal enhancer of split (AES) (NM_001130) Human Untagged Clone

CAT#: SC119415

AES (untagged)-Human amino-terminal enhancer of split (AES), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Amino terminal enhancer of split
• Synonyms: AES; AES-1; AES-2; ESP1; GRG; Grg-5; GRG5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119415 sequence for NM_001130 edited (data generated by NextGen Sequencing)
  ATGATGTTTCCACAAAGCAGGCATTCGGGCTCCTCGCACCTACCCCAGCAACTCAAATTC
  ACCACCTCGGACTCCTGCGACCGCATCAAAGACGAATTTCAGCTACTGCAAGCTCAGTAC
  CACAGCCTCAAGCTCGAATGTGACAAGTTGGCCAGTGAGAAGTCAGAGATGCAGCGTCAC
  TATGTGATGTACTACGAGATGTCCTACGGCTTGAACATCGAGATGCACAAACAGGCTGAG
  ATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCTGCCCTACCTCTCCCAAGAGCAC
  CAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCAGGTCACCGCTCCCGAGCTGAAC
  TCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTCCCAGCTGCAGGCCCTGGCCCTG
  CCCTTGACCCCACTACCCGTGGGGCTGCAGCCGCCTTCGCTGCCGGCGGTCAGCGCAGGC
  ACCGGCCTCCTCTCGCTGTCCGCGCTGGGTTCCCAGGCCCACCTCTCCAAGGAAGACAAG
  AACGGGCACGATGGTGACACCCACCAGGAGGATGATGGCGAGAAGTCGGATTAG
  
  Clone variation with respect to NM_001130.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001130 unedited
  TAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGATCGGCGCCGGGACAA
  AGGCGCGGCCGCCCGGCGCCCCCAGCAGCCCGAGCCGGGGCGCACAGCCGGGGCGCAGCC
  CGCGCCCCCCGCCGCGATTGACATGATGTTTCCACAAAGCAGGCATTCGGGCTCCTCGCA
  CCTACCCCAGCAACTCAAATTCACCACCTCGGACTCCTGCGACCGCATCAAAGACGAATT
  TCAGCTACTGCAAGCTCAGTACCACAGCCTCAAGCTCGAATGTGACAAGTTGGCCAGTGA
  GAAGTCAGAGATGCAGCGTCACTATGTGATGTACTACGAGATGTCCTACGGCTTGAACAT
  CGAGATGCACAAACAGGCTGAGATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCT
  GCCCTACCTCTCCCAAGAGCACCAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCA
  GGTCACCGCTCCCGAGCTGAACTCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTC
  CCAGCTGCAGGCCCTGGCCCTGCCCTTGACCCCACTACCCGTGGGGCTGCAGCCGCCTTC
  GCTGCCGGCGGTCAGCGCAGGCACCGGCCTNCTCTCGCTGTCCGCGCTGGGTTCCCAGGC
  CCACCTCTCCAGGGAGACAGAACGGGCACGATGGTGACACCCACCAGGAGGATGATGGCG
  AGAAAGTCGATTANCAAGGNGGCCGGACGGNGAGGGTTGGAGGGNGGACCAGAGGGANAC
  AGAGGCACNGAGAGAAAAGGATGTTTATCACAAGACACAGCGGAGCTCGGGGATGGNCTA
  ACTCCATAGTATTTATGGTGGCCGNNCGCGNNGGCCCCAGCCAGCTTGCAAGCCCACTNT
  AGCTTTNTTNNCTACCCCATACGGNNNTTCTTCCTCCTCCCNTGCAGNCTGGNTATGTGG
  ATACCTGCCCTGACTGGTTGAGCAAGCTAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001130 unedited
  CGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACT
  TCTCACATCTTTATTGGAAAGGCACAGCTAAGCCCACCCTTGATACAGCATTTTCCACTT
  CTCTCTGTAGAGATCAGACGATTGAACACGAGATAAGCTGACTATATACAGACACAGGTG
  TGGGTGTTGCTTTTTTTTAAAAACACTTTTGGCTTTTTTTTTTTTTAATATCCCCTTTCT
  TAAAAGACAAGCTAGTATACTGGAAAAAGAAAAAAATAATAATAAAATAAAAACCAAGAC
  AACTTTAGTACCCTCATCTTTATTTGGGAAGGGGAGGGGGAATCCTGGGTCGCCCACCCT
  TACCCTGGTCCTCCCAGCTCAGCTAAGCTCGTCCCTCGTGCCCCCCCTTTTGTGGGCGAT
  GGGAAAGGACCAGGTGGGCGTGGAGGTGTCTGGAACTAACAAAGGTGGTGAGTGGGGCAA
  GTGGAGGTGGGAGCATACCTGGGACCCGGGGTCGGGGGAGACTCGGGGTGCCCAGGACGG
  GAAAGGGGCAGCTAGCATTGCGTGCATGCAGTACCAGGGTGAGAGGGCTGTGGCCCAGGC
  AGACTGTCGGTTACACATGTTCAAAACGGGGGAAGGGCCGGGGCTGCTGCGCTTCGCGAG
  GTCTTGCTCCCTTGGGACCTGGTCTCCCATCTGACCCTCCAGGCCTTAGCTTGCCTCACA
  CGTCAGGGCAGGTATCCACCTAACGGNCTGCAGGGGAGGAGGAGGGAANCCCGGATGGGG
  TANGAAAAAGCTANAGTGGCCTGCAACTGGCTGGGGCCCCGCCGCGGCCCATAATACTTG
  GGATTATCCATCCGACTCGCTGGTCTGGCTAAATTCTCTTCGCGCCCGGGTCCCCCTTGC
  CCCCTTCAACTCCGGTCGGCCCTGTATACGATTCGCTATCCCTGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001130
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130.5, NP_001121.2
• RefSeq Size: 1687 bp
• RefSeq ORF: 594 bp
• Locus ID: 166
• UniProt ID: Q08117
• Cytogenetics: 19p13.3
• Domains: TLE_N
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus compared to isoform a.