BMPR1B (NM_001203) Human Untagged Clone

CAT#: SC119393

BMPR1B (untagged)-Human bone morphogenetic protein receptor, type IB (BMPR1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BMPR1B
• Synonyms: ALK-6; ALK6; AMDD; BDA1D; BDA2; CDw293
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119393 sequence for NM_001203 edited (data generated by NextGen Sequencing)
  ATGCTTTTGCGAAGTGCAGGAAAATTAAATGTAGGCACCAAGAAAGAGGATGGTGAGAGT
  ACAGCCCCCACCCCCCGTCCAAAGGTCTTGCGTTGTAAATGCCACCACCATTGTCCAGAA
  GACTCAGTCAACAATATTTGCAGCACAGACGGATATTGTTTCACGATGATAGAAGAGGAT
  GACTCTGGGTTGCCTGTGGTCACTTCTGGTTGCCTAGGACTAGAAGGCTCAGATTTTCAG
  TGTCGGGACACTCCCATTCCTCATCAAAGAAGATCAATTGAATGCTGCACAGAAAGGAAC
  GAATGTAATAAAGACCTACACCCTACACTGCCTCCATTGAAAAACAGAGATTTTGTTGAT
  GGACCTATACACCACAGGGCTTTACTTATATCTGTGACTGTCTGTAGTTTGCTCTTGGTC
  CTTATCATATTATTTTGTTACTTCCGGTATAAAAGACAAGAAACCAGACCTCGATACAGC
  ATTGGGTTAGAACAGGATGAAACTTACATTCCTCCTGGAGAATCCCTGAGAGACTTAATT
  GAGCAGTCTCAGAGCTCAGGAAGTGGATCAGGCCTCCCTCTGCTGGTCCAAAGGACTATA
  GCTAAGCAGATTCAGATGGTGAAACAGATTGGAAAAGGTCGCTATGGGGAAGTTTGGATG
  GGAAAGTGGCGTGGCGAAAAGGTAGCTGTGAAAGTGTTCTTCACCACAGAGGAAGCCAGC
  TGGTTCAGAGAGACAGAAATATATCAGACAGTGTTGATGAGGCATGAAAACATTTTGGGT
  TTCATTGCTGCAGATATCAAAGGGACAGGGTCCTGGACCCAGTTGTACCTAATCACAGAC
  TATCATGAAAATGGTTCCCTTTATGATTATCTGAAGTCCACCACCCTAGACGCTAAATCA
  ATGCTGAAGTTAGCCTACTCTTCTGTCAGTGGCTTATGTCATTTACACACAGAAATCTTT
  AGTACTCAAGGCAAACCAGCAATTGCCCATCGAGATCTGAAAAGTAAAAACATTCTGGTG
  AAGAAAAATGGAACTTGCTGTATTGCTGACCTGGGCCTGGCTGTTAAATTTATTAGTGAT
  ACAAATGAAGTTGACATACCACCTAACACTCGAGTTGGCACCAAACGCTATATGCCTCCA
  GAAGTGTTGGACGAGAGCTTGAACAGAAATCACTTCCAGTCTTACATCATGGCTGACATG
  TATAGTTTTGGCCTCATCCTTTGGGAGGTTGCTAGGAGATGTGTATCAGGAGGTATAGTG
  GAAGAATACCAGCTTCCTTATCATGACCTAGTGCCCAGTGACCCCTCTTATGAGGACATG
  AGGGAGATTGTGTGCATCAAGAAGTTACGCCCCTCATTCCCAAACCGGTGGAGCAGTGAT
  GAGTGTCTAAGGCAGATGGGAAAACTCATGACAGAATGCTGGGCTCACAATCCTGCATCA
  AGGCTGACAGCCCTGCGGGTTAAGAAAACACTTGCCAAAATGTCAGAGTCCCAGGACATT
  AAACTCTGA
  
  Clone variation with respect to NM_001203.2
  33 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001203 unedited
  ACGCGGGAGCCGGGAGCGCAGCCGCGGGGTGGAGTTCAGCCTACTCTTTCTTAGATGTGA
  AAGGAAAGGAAGATCATTTCATGCCTTGTTGATAAAGGTTCAGACTTCTGCTGATTCATA
  ACCATTTGGCTCTGAGCTATGACAAGAGAGGAAACAAAAAGTTAAACAAGCAAGCCTGCC
  ATAAGTGAGAAGCAAACTTCCTTGATAACATGCTTTTGCGAAGTGCAGGAAAATTAAATG
  TAGGCACCAAGAAAGAGGATGGTGAGAGTACAGCCCCCACCCCCCGTCCAAAGGTCTTGC
  GTTGTAAATGCCACCACCATTGTCCAGAAGACTCAGTCAACAATATTTGCAGCACAGACG
  GATATTGTTTCACGATGATAGAAGAGGATGACTCTGGGTTGCCTGTGGTCACTTCTGGTT
  GCCTAGGACTAGAAGGCTCACATTTTCAGTGTCGGGACACTCCCATTCCTCATCAAAGAA
  GATCAATTGAATGCTGCACAGAAAGGAACGAATGTGATAAAGACCTACACCCTACACTGC
  CTCCATTGAAAAAACAGAGATTTGTTGATGGACCTATACACCACAGGGCTTTACTTATAT
  CTGTGACTGACTGAAATTTGCTCTTGGGCCTAACATAATATTGTGGTACTTCCGGGTTAA
  AGACAAGAAAACCGACCTTGATCCACATTGGGGTATAACAGGATGAAAATTAATTTCTCC
  TGGAGAATCCCTGAAAGACTTATTGACCATTTCATAACTAAGAAGGGCACCGGCCTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001203 unedited
  AAAATCTGACTTGANACCGCGGCCGCATTCTAGNGATCNGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTCCAGGGGGAAAATGAAGCCTTTTTATTTAGGGGAATGTTAAAACA
  AAGCCATTTACAATTTGTAACAAATACAATACAATTGTTGGTTCCCTAATCCTGAATAAT
  TACAGTATATACAAAATTTGTAAGGCACTGGTTCCCCCAAAAAAACAAATGAGTCTTTTT
  GGGAAACAAACAACGAAACCCCTATATTGCCAACTTTTCTCCAAAAAATAGGGCATTGTT
  TTTTAGTTTTGTTTTACAAGAGGAATCACAATTGCTAAATTAAGTCACTTCTGATGCCAT
  TTCTTTCATTTCCACTGCTATGTGTTTGGGTTTTCTAAGGCAATGGAACTACTGAACATG
  TTTAAGTTTAAAAAATTATTCTTTTTTCAGTGCCGTACCACCCACAAACAAACAATAATC
  ATATCGAACCAACAACATAAAACTTCTAACGAATATGTTTGACTGCAGGAACATAAAATT
  AAGTAATTTATAAGACCTTCGCTGACCTTGACCTCCCACCAGTTGAAATGTGGAAACACA
  ATTCCGTGCAAAAAAAATCTACACGTTCTTTCTAAAAACCTTCGGTTTGGGGAATTCAAA
  AATGGGCCCTTTTGATTTTGGGAATGTGCTTCACTCAAGGCCAAAGGCCTCTCTCCTTCC
  ACCCAGTGGGCAAAGCACCTTCTCTTTTTTCTCCACATCTAACACCCTTTTTTAAAACAC
  CCTTTGGCCCCTTCCTCGTTTTTATAACCGTAAATATTTACTCTTTCCTCCATTTTTGCC
  ATCTGCGCCGGCGTTACTAATTGATTTTGTTATCTTCCGCCACTGACTTCCCAATTTAAC
  ACCCTATGAGATAATTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001203
• Insert Size: 6000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001203.1, NP_001194.1
• RefSeq Size: 2032 bp
• RefSeq ORF: 1509 bp
• Locus ID: 658
• UniProt ID: O00238
• Cytogenetics: 4q22.3
• Domains: Activin_recp, pkinase, TyrKc, S_TKc, GS
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
• Gene Summary: This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.