DGKA (NM_001345) Human Untagged Clone

CAT#: SC119317

DGKA (untagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGKA
• Synonyms: DAGK; DAGK1; DGK-alpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119317 sequence for NM_001345 edited (data generated by NextGen Sequencing)
  ATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATACATG
  GAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGATGGCT
  AAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAATCTAT
  CTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGAGACT
  GGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGTGGTGTGTCTCAATGATGTTTCC
  TGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCTTCAAG
  CTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTATCCTA
  CAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGATTCTT
  CAGGAGATGATGAAAGAGATTGACTATGATGGCAGTGGCTCTGTCTCTCAAGCTGAGTGG
  GTCCGGGCTGGGGCCACCACCGTGCCACTGCTAGTGCTGCTGGGTCTGGAGATGACTCTG
  AAGGACGACGGACAGCACATGTGGAGGCCCAAGAGGTTCCCCAGACCAGTCTACTGCAAT
  CTGTGCGAGTCAAGCATTGGTCTTGGCAAACAGGGACTGAGCTGTAACCTCTGTAAGTAC
  ACTGTTCACGACCAGTGTGCCATGAAAGCCCTGCCTTGTGAAGTCAGCACCTATGCCAAG
  TCTCGGAAGGACATTGGTGTCCAATCACATGTGTGGGTGCGAGGAGGCTGTGAGTCCGGG
  CGCTGCGACCGCTGTCAGAAAAAGATCCGGATCTACCACAGTCTGACCGGGCTGCATTGT
  GTATGGTGCCACCTAGAGATCCACGATGACTGCCTGCAAGCGGTGGGCCATGAGTGTGAC
  NNNNNNCTGCTCCGGGATCACATCCTGCCTCCATCTTCCATCTATCCCAGTGTCCTGGCC
  TCTGGACCGGATCGTAAAAATAGCAAAACAAGCCAGAAGACCATGGATGATTTAAATTTG
  AGCACCTCTGAGGCTCTGCGGATTGACCCTGTTCCTAACACCCACCCACTTCTCGTCTTT
  GTCAATCCTAAGAGTGGCGGGAAGCAGGGNNNNAGGGTGCTCTGGAAGTTCCAGTATATA
  TTAAACCCTCGACAGGTGTTCAACCTCCTAAAGGATGGTCCTGAGATAGGGCTCCGATTA
  TTCAAGGATGTTCCTGATAGCCGGATTTTGGTGTGTGGTGGAGACGGCACAGTAGGCTGG
  ATTCTAGAGACCATTGACAAAGCTAACTTGCCAGTTTTGCCTCCTGTTGCTGTGTTGCCC
  CTGGGTACTGGAAATGATCTGGCTCGATGCCTAAGATGGGGAGGAGGTTATGAAGGACAG
  AATCTGGCAAAGATCCTCAAGGATTTAGAGATGAGTAAAGTGGTACATATGGATCGATGG
  TCTGTGGAGGTGATACCTCAACAAACTGAAGAAAAAAGTGACCCAGTCCCCTTTCAAATC
  ATCAATAACTACTTCTCTATTGGCGTGGATGCCTCTATTGCTCATCGATTCCACATCATG
  CGAGAGAAATATCCGGAGAAGTTCAACAGCAGAATGAAGAACAAGCTATGGTACTTCGAA
  TTTGCCACATCTGAATCCATCTTCTCAACATGCAAAAAGCTGGAGGAGTCTTTGACAGTT
  GAGATCTGTGGGAAACCGCTGGATCTGAGCAACCTGTCCCTAGAAGGCATCGCAGTGCTA
  AACATCCCTAGCATGCATGGTGGCTCCAACCTCTGGGGTGATACCAGGAGACCCCATGGG
  GATATCTATGGGATCAACCAGGCCTTAGGTGCTACAGCTAAAGTCATCACCGACCCTGAT
  ATCCTGAAAACCTGTGTACCAGACCTAAGTGACAAGAGACTGGAAGTGGTTGGGCTGGAG
  GGTGCAATTGAGATGGGCCAAATCTATACCAAGCTCAAGAATGCTGGACGTCGGCTGGCC
  AAGTGCTCTGAGATCACCTTCCACACCACAAAAACCCTTCCCATGCAAATTGACGGAGAA
  CCCTGGATGCAGACGCCCTGTACAATCAAGATCACCCACAAGAACCAGATGCCCATGCTC
  ATGGGCCCACCCCCCCGCTCCACCAATTTCTTTGGCTTCTTGAGCTAA
  
  Clone variation with respect to NM_001345.4
  961 t=>n;962 g=>n;963 t=>n;964 g=>n;965 g=>n;966 g=>n;1170 g=>n;1171 c=>n;1172 a=>n;1173 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001345 unedited
  GTAATTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGAGTGAGTCCC
  TAGGCCTCCATCTCTCTCCCTTGCTGTACCACCTTCACCACCATCCATGCGACCCCAAGA
  GCCTTAATGACTCTAGAAGAGACTCCAGGCAGGGGAAGCTGAAAGGACCTTTCACTCCCT
  ACTTTTGGCCAGGGCCTTCTGTGCCACCTGCCAAGACCAGCAGGCCTACCCTCTGAAGAG
  GTCCAAGCAACGGAAGTACTACTACGAAGCTGCCTTTCTGGCCATCCTTGAGAAAAATAG
  ACAGATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATA
  CATGGAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGAT
  GGCTAAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAAT
  CTATCTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGA
  GACTGGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGGTGGTGTGTCTCAATGATG
  TTTCCTGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCT
  TCAAGCTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTA
  TCCTACAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGA
  TTCTTCAGGAGATGATGAAGAGATGCTATGATGGCAGGGGCTCTGTTCTCAACTGGAGGG
  GTCCGGGCTGGGGCCACACCGTGCCCTGCTATGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001345 unedited
  GGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGATCCCAAGGGGGAAACA
  CAAGTCATTTTATTCAGATGAGGCACTTTCAATGTATGTGTTTTGGGGTGTGTGTATGTG
  TGTGCGCATGGGGAACAGTGAGGTGGTGCAGGATAATAAATACTGTGAGGGTGAAGGAAC
  ACTCCCCCAAGCTGGCAGGAGTATGTGGCAGCAATGTACAGAGCCTCGGGAGTAGAGTCC
  AGGGACAGGGAGGTGGGTTCAAGGCTGGCTTGGAGGCCAAGGGTGTCCCCCTTAGCTCAA
  GAAGCCAAAAAAATTGGTGGAGCGGGGGGGTGGGCCCATGAGCATGGGCATCTGGTTCTT
  GTGGGTGATCTTGATTGTACAGGGCGTCTGCATCCAGGGTTCTCCGTCAATTTGCATGGG
  AAGGGTTTTTGTGGTGTGGAAGGTGATCTCAGAGCACTTGGCCAGCCGACGTCCAGCATT
  CTTGAGCTTGGTATAGATTTGGCCCATCTCAATTGCACCCTCCAGCCCAACCACTTCCAG
  TCTTTTGTTACTTACGTCTGGTACACAGGTTTTCAGGATATCACGGTCGGTGATGACTTT
  AACTGAAGCACCTAACGGCTGGTTGATCCCATATATATCCCCCATGGCGCGCTCCTGGTA
  TCACCTCCTAAACGCCGGAGCCCCCATGCGATTGTTAGGGATGGTTACGACCTGCGAACG
  CCTCTCCAGGGACAGGGTGCCCAAACCCCCCGGTTTCCCCACACATCTCCCTCCCCCAAC
  ACTTCCTTCGTTCTTTTATATGTCGGAAACGAACCCTGTTACCATCCCCAAACANGCGAA
  GACATANATAGTGTTCTTTATTTCCTTCCTCTATCTTTGTGGGAAATCGGTGTGGAATTG
  GGGGAGAGAGGGGCGCCCCGGCCGCGCCCTCCCATGACATTTCACTCCACCTTCTTCCGC
  CCCCCCCATTCGCCCTCCGTCTTCCCCTCCGCCCTCCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001345
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001345.4, NP_001336.2
• RefSeq Size: 2756 bp
• RefSeq ORF: 2208 bp
• Locus ID: 1606
• UniProt ID: P23743
• Cytogenetics: 12q13.2
• Domains: DAGKa, DAGKc, EFh, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]
  Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a.