Chromogranin B (CHGB) (NM_001819) Human Untagged Clone

CAT#: SC119004

CHGB (untagged)-Human chromogranin B (secretogranin 1) (CHGB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Chromogranin B
• Synonyms: SCG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119004 sequence for NM_001819 edited (data generated by NextGen Sequencing)
  ATGCAGCCAACGCTGCTTCTCAGCCTCCTGGGAGCCGTGGGGCTGGCGGCTGTCAATTCC
  ATGCCAGTGGATAACAGGAACCACAATGAAGGAATGGTGACTCGCTGCATCATTGAGGTC
  CTCTCAAATGCCTTGTCGAAGTCCAGCGCTCCACCCATCACCCCTGAGTGCCGCCAAGTC
  CTGAAGACGAGTAGAAAAGACGTCAAAGACAAAGAGACAACTGAAAATGAAAACACAAAG
  TTTGAAGTAAGATTGTTAAGAGACCCAGCTGATGCCTCGGAAGCCCACGAGTCCTCCAGC
  AGGGGAGAGGCAGGAGCCCCAGGGGAGGAGGACATCCAAGGCCCAACAAAGGCAGACACA
  GAGAAATGGGCAGAGGGAGGCGGGCACAGCCGAGAGCGAGCGGATGAGCCCCAGTGGAGC
  CTCTATCCCTCCGACAGCCAAGTCTCTGAAGAAGTGAAGACACGCCATTCTGAGAAGAGC
  CAGAGAGAGGATGAGGAGGAGGAGGAGGGAGAGAACTATCAAAAAGGGGAGCGAGGGGAA
  GATAGCAGTGAAGAGAAACACCTTGAAGAGCCAGGAGAGACACAAAACGCTTTTCTCAAT
  GAAAGAAAGCAGGCTTCAGCTATAAAAAAAGAGGAGTTAGTGGCCAGATCGGAAACACAT
  GCTGCCGGGCATTCTCAGGAGAAGACACATAGCCGAGAGAAGAGTAGCCAGGAGAGTGGA
  GAGGAGACAGGGAGCCAGGAGAATCACCCCCAGGAGTCTAAAGGCCAACCCCGAAGCCAG
  GAAGAATCTGAGGAAGGTGAGGAAGATGCCACCTCTGAGGTGGACAAACGACGCACGAGG
  CCCAGACACCACCACGGGAGGAGCAGGCCCGACAGGTCCTCTCAAGGAGGGAGTCTTCCC
  TCTGAGGAAAAGGGACACCCCCAGGAGGAATCTGAGGAGTCAAACGTCAGCATGGCCAGT
  TTAGGGGAAAAGAGGGACCACCATTCAACCCACTACAGGGCTTCAGAGGAAGAACCTGAA
  TATGGAGAAGAAATAAAGGGTTATCCAGGCGTCCAGGCCCCTGAGGACCTGGAGTGGGAG
  CGCTATAGGGGCAGAGGAAGTGAAGAATACAGGGCTCCAAGACCTCAGAGTGAGGAGAGT
  TGGGATGAGGAGGACAAGAGAAACTACCCCAGCTTAGAGCTTGATAAGATGGCACATGGA
  TATGGTGAAGAAAGTGAGGAAGAGAGGGGCCTTGAGCCGGGAAAGGGACGCCATCACAGA
  GGCAGGGGAGGGGAGCCACGTGCCTATTTCATGTCTGACACCAGAGAAGAGAAAAGGTTC
  TTGGGTGAAGGACACCACCGTGTCCAAGAAAACCAGATGGACAAGGCAAGGAGGCATCCA
  CAAGGTGCGTGGAAAGAGCTGGACAGAAATTATCTCAACTACGGTGAGGAAGGAGCCCCA
  GGGAAGTGGCAGCAGCAGGGAGACCTGCAGGACACTAAAGAAAACAGGGAGGAAGCTAGG
  TTTCAAGATAAACAATATAGCTCCCATCACACAGCTGAAAAGAGGAAGAGATTAGGGGAA
  CTGTTCAACCCATACTACGACCCTCTCCAGTGGAAGAGCAGCCATTTTGAAAGAAGAGAC
  AACATGAATGACAATTTTCTCGAGGGTGAGGAGGAAAATGAGCTGACCTTGAACGAGAAG
  AATTTCTTCCCAGAATACAACTATGACTGGTGGGAGAAAAAGCCCTTCTCTGAGGATGTG
  AACTGGGGGTATGAGAAGAGAAACCTCGCCAGGGTCCCCAAGCTGGACCTGAAAAGGCAA
  TATGACAGGGTGGCCCAACTGGACCAGCTCCTTCACTACAGGAAGAAGTCAGCTGAGTTT
  CCAGACTTCTATGATTCTGAGGAGCCGGTGAGCACCCACCAGGAGGCAGAAAATGAAAAG
  GACAGGGCTGACCAGACAGTCCTGACAGAGGACGAGAAAAAAGAACTCGAAAACTTGGCT
  GCAATGGATTTGGAACTACAGAAGATAGCTGAGAAATTCAGCCAAAGGGGCTGA
  
  Clone variation with respect to NM_001819.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001819 unedited
  TGCAGTATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTTTTCCGGG
  GCCGCTCCATCGCGCCTTCCTCCTGCGCCTCGCTTCTCCGGTCCAGCCGCCATCTTCCTT
  TCCGCACAGGGGCCGCCGAGCGGGGCCATGCAGCCAACGCTGCTTCTCAGCCTCCTGGGA
  GCCGTGGGGCTGGCGGCTGTCAATTCCATGCCAGTGGATAACAGGAACCACAATGAAGGA
  ATGGTGACTCGCTGCATCATTGAGGTCCTCTCAAATGCCTTGTCGAAGTCCAGCGCTCCA
  CCCATCACCCCTGAGTGCCGCCAAGTCCTGAAGACGAGTAGAAAAGACGTCAAAGACAAA
  GAGACAACTGAAAATGAAAACACAAAGTTTGAAGTAAGATTGTTAAGAGACCCAGCTGAT
  GCCTCGGAAGCCCACGAGTCCTCCAGCAGGGGAGAGGCAGGAGCCCCAGGGGAGGAGGAC
  ATCCAAGGCCCAACAAAGGCAGACACAGAGAAATGGGCAGAGGGAGGCGGGCACAGCCGA
  GAGCGAGCGGATGAGCCCCAGTGGAGCCTCTATCCCTCCGACAGCCAAGTCTCTGAAGAA
  GTGAAGACACGCCATTCTGAGAAGAGCCAGAGAGAGGATGAGGAGGAGGAGGAGGGAGAG
  AACTATCAAAAAGGGGAGCGAGGGGAAGATAGCAGTGAAGAGAACACCTTGAAGAGCCCA
  GAGAGACATCAAACGCTTTNCTCAATGAAAGAAAGCAGGCTTCAGCTATAAAAAAAGAAG
  AGTTAGTGGCCAGATTCGGAACACATGCTGNCGGGCATTCTCAGGAGATGACACATAGCC
  CGAAAAAAGAGTANCCAGCAAAGTGGAGAGGAGACAGGAACCCAGGATATCACCCCCAGG
  AGTCTAAAGGCCACCCCCGAACCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001819 unedited
  TTTATTATTTTCCAACTGGANACCAAACATTTTTCCAAAAACAGTAGCACAGTCATTGTC
  ATAAGCATGTTAATATGTCTATTTTGCAGAAAAGAATATGCTACAAGTCATGCAATTCAC
  ATTTTCAATAGCATTCTGACAGAAATTAAAGACAATTCCAATTGTGTCAAACATTTAATG
  AATAGTAAGTTCTACTTTCTGCCCTTGGGTAGATAAATGGTGTCTTTCAGTGAAGTGGTG
  AAAAACAGATCATGTGATGGCTGCTTCTTAACAGTGCCCACCGCTCCAATGACAGTCAGC
  CCCTTTGGCTGAATTTCTCAGCTATTCTTCTGTAGTTCCAAATCCATTGCAGCCAAGTTT
  TCGAGTTCTTTTTTCTCGTCCTCTGTCAGGACTGTCTGGTCAGCCCTGTCCTTTTCATTT
  TCTGCCTCCTGGTGGGTGCTCACCGGCTCCTCAGAATCATAGAAGTCTGGAAACTCAGCT
  GACTTCTTCCTGTAGTGAAGGAGCTGGTCCAGTTGGGCCACCCTGTCATATTGCCTTTTC
  AGGTCCAGCTTGGGGACCCTGGCGAGGTTTCTCTTCTCATACCCNCAGTTCACATCCTCA
  GAGAAGGGCTTTNTCTCCCACAGTCATAGTTGTATTCTGGGAAGAATNNNCTCTCGTCAA
  GTCAGCTCATTTTCTNCTCACCCTCGAGAAAATGNCATTCATGTNGNCTCTTCTTCAAAT
  GGCTGCTCTTCACTGGAGAGGGTCGTNTATGGGTTGACAGNTCCCCTAATCTCTTCTCTT
  TCAGCTGTGTGATGGAGCTATATGGTTATCTTGAACCTAGCTCCTNCCTGGTTTCTTATT
  GCCTGCAGGTCTTCTGCTGCTGCCCTTTCCTGGGGCTCCTTTCTACGAATTGAAATATTC
  TGTCACTCTTCCACGCACTGGGGATGCCTCTGGCTGCCATCTGGTTCTGGACCGGGGGGC
  CTTACCAAGACCTTTCTTCCTTGTGTAGATAAAAGCCCGGGCTCCTCCTGCCTTGTAGGG
  GTCTTTCGGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001819
• Insert Size: 2630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001819.1, NP_001810.1
• RefSeq Size: 2454 bp
• RefSeq ORF: 2034 bp
• Locus ID: 1114
• UniProt ID: P05060
• Cytogenetics: 20p12.3
• Domains: Granin
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a tyrosine-sulfated secretory protein abundant in peptidergic endocrine cells and neurons. This protein may serve as a precursor for regulatory peptides. [provided by RefSeq, Jan 2009]