Carboxypeptidase H (CPE) (NM_001873) Human Untagged Clone

CAT#: SC118995

CPE (untagged)-Human carboxypeptidase E (CPE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Carboxypeptidase H
• Synonyms: CPH; IDDHH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118995 sequence for NM_001873 edited (data generated by NextGen Sequencing)
  ATGGCCGGGCGAGGGGGCAGCGCGCTGCTGGCTCTGTGCGGGGCACTGGCTGCCTGCGGG
  TGGCTCCTGGGCGCCGAAGCCCAGGAGCCCGGGGCGCCCGCGGCGGGCATGAGGCGGCGC
  CGGCGGCTGCAGCAAGAGGACGGCATCTCCTTCGAGTACCACCGCTACCCCGAGCTGCGC
  GAGGCGCTCGTGTCCGTGTGGCTGCAGTGCACCGCCATCAGCAGGATTTACACGGTGGGG
  CGCAGCTTCGAGGGCCGGGAGCTCCTGGTCATCGAGCTGTCCGACAACCCTGGCGTCCAT
  GAGCCTGGTGAGCCTGAATTTAAATACATTGGGAATATGCATGGGAATGAGGCTGTTGGA
  CGAGAACTGCTCATTTTCTTGGCCCAGTACCTATGCAACGAATACCAGAAGGGGAACGAG
  ACAATTGTCAACCTGATCCACAGTACCCGCATTCACATCATGCCTTCCCTGAACCCAGAT
  GGCTTTGAGAAGGCAGCGTCTCAGCCTGGTGAACTCAAGGACTGGTTTGTGGGTCGAAGC
  AATGCCCAGGGAATAGATCTGAACCGGAACTTTCCAGACCTGGATAGGATAGTGTACGTG
  AATGAGAAAGAAGGTGGTCCAAATAATCATCTGTTGAAAAATATGAAGAAAATTGTGGAT
  CAAAACACAAAGCTTGCTCCTGAGACCAAGGCTGTCATTCATTGGATTATGGATATTCCT
  TTTGTGCTTTCTGCCAATCTCCATGGAGGAGACCTTGTGGCCAATTATCCATATGATGAG
  ACGCGGAGTGGTAGTGCTCACGAATACAGCTCCTCCCCAGATGACGCCATTTTCCAAAGC
  TTGGCCCGGGCATACTCTTCTTTCAACCCGGCCATGTCTGACCCCAATCGGCCACCATGT
  CGCAAGAATGATGATGACAGCAGCTTTGTAGATGGAACCACCAACGGTGGTGCTTGGTAC
  AGCGTACCTGGAGGGATGCAAGACTTCAATTACCTTAGCAGCAACTGTTTTGAGATCACC
  GTGGAGCTTAGCTGTGAGAAGTTCCCACCTGAAGAGACTCTGAAGACCTACTGGGAGGAT
  AACAAAAACTCCCTCATTAGCTACCTTGAGCAGATACACCGAGGAGTTAAAGGATTTGTC
  CGAGACCTTCAAGGTAACCCAATTGCGAATGCCACCATCTCCGTGGAAGGAATAGACCAC
  GATGTTACATCCGCAAAGGATGGTGATTACTGGAGATTGCTTATACCTGGAAACTATAAA
  CTTACAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGGCAGTTCCTTACAGC
  CCTGCTGCTGGGGTTGATTTTGAACTGGAGTCATTTTCTGAAAGGAAAGAAGAGGAGAAG
  GAAGAATTGATGGAATGGTGGAAAATGATGTCAGAAACTTTAAATTTTTAA
  
  Clone variation with respect to NM_001873.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001873 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCGGACGCCAT
  TTGGCTGTTGACGTGGTTCCGAGCCAGCAAATAACGCCAGCAGCCCTCCCAGATCCACGC
  CGGCCCGTCTCTCCGCCGGCCCCCTCCTCGCAGTGGTTTCTCCTGCAGCTCCCCTGGGCT
  CCGCGGCCAGTAGTGCAGCCCGTGGAGCCGCGGCTTTGCCCGTCTCCTCTGGGTGGCCCC
  AGTGCGCGGGCTGACACTCATTCAGCCGGGGAAGGTGAGGCGAGTAGAGGCTGGTGCGGA
  ACTTGCCGCCCCCAGCAGCGCCGGCGGGCTAAGCCCAGGGCCGGGCAGACAAAAGAGGCC
  GCCCGCGTAAGAAGGCACGGCCGGCGGCGGCGGAGCGCATCGATGGCCGGGCGAGGGGGC
  AGCGCGCTGCTGGCTCTGTGCGGGGCACTGGCTGCCTGCGGGTGGCTCCTGGGCGCCGAA
  GCCCAGGAGCCCGGGGCGCCCGCGGCGGGCATGAGGCGGCGCCGGCGGCTGCAGCAAGAG
  GACGGCATCTCCTTCGAGTACCACCGCTACCCCGAGCTGCGCGAGGCGCTCGTGTCCGTG
  TGGCTGCAGTGCACCGCCATCAGCAGGATTTACACGGTGGGGCGCAGCTTCGAGGGCCGG
  GAGCTCCTGGTCATCGAGCTGTCCGACAACCCTGGCGTCCATGAGCCTGGTGAGCCTGAA
  TTTAAATACATTGGGAATATGCATGGGAATGAGGCTGTTGGACGAGAACTGCTCATTNTC
  TTGCCCAGTACCCTATGCACGAATACCAGAGGGGAACGAGACANNTGTCACCTGATCACA
  GTACCCGCATTACATCATGCCTTCCCTGAAACCAGATGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001873 unedited
  TAGTCGAGTTTTTTTTTCTTTTTTTTAACCTTTATTCATTCAGATTGCAATTCTGGGCCA
  CTCTACAACCAAGAGAAAACCCTAAACTTTTAAGTAGTGTTAACGGACTACATAATATAA
  TAGTATTGCTCCTATATGTACAAGCAAGAAGTCAATTTTTATTCTTTTCATAAAACCTCT
  TTGTAAACATGTACCTTTTCTTCCCATCAAAAATGCATTAACAAGATCATGCTTATAGTT
  AGTCAGCACAAGAAAAAAACGACAATTTATACTGAACTATTTAAGTCTTGCAGTAGAGTG
  CTCACAACTCCGAGCTGTATCTGTTAACCTTTTCAATAGCATTCATTATGTGACATTGTC
  ACCAATTACAGTAAAAGAACTTCACTAATTTTTGAAAGCTAGAATTACTCAAAAAATTCT
  GCATTGTAAATAATACCAGGAATATTGCATTTAAGCCTAGGATGGCAAAATTACTTAAAT
  CTTTTCTATACTTTTTTGTGTAATATAGGTAGGAAAATGAATACTATATAAGAGAATGAA
  ATATATCAAGTTCTTTATATTTTTACCTAAGAGGCTATTTATCTTAAGGAAAGTTGATTA
  ATATTTAATGATTAAAAATAAATCAATGTTAGGATTAACTGCACAAAATCTAAAAAAAGA
  CACCTTACATCATACTACTTATATAGATAGATTTAAAGCAGCTACCTAGAGCCCTTTTAA
  AAATTAAAGTTTTGGACNTATTTTCCACCATTCATCAATGTTTCCTCTCCCTTCTTTCCT
  TTAAAAAAGGACTCCCTTCAAAACAACCCCAACAGCAGGGCTGTAAGAACCGCCACCTTT
  CTTTGTTTTCGCCAATACCCGGAACTGAAGCCTGAACTTATAATTTCAAGGGTTAACACA
  TTCCACATAATCCCATCTCTTGGGAAGTAACACGTGGGCTTTTCTCTTCACGGGAATGGG
  GGCTCCCAATTGCGTTCACTCTAAGAGCCGGGACAATCTTTATACTCTCGCGGATTTGTC
  TAGGACACAAGAGGGGAATNTTTGTTCCCTCCAAAGATCCTCAGATTCTTCGCGGGAGAC
  TCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001873
• Insert Size: 2560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001873.1, NP_001864.1
• RefSeq Size: 2443 bp
• RefSeq ORF: 1431 bp
• Locus ID: 1363
• UniProt ID: P16870
• Cytogenetics: 4q32.3
• Domains: Zn_carbOpept
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Type I diabetes mellitus
• Gene Summary: This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015]