DLST (NM_001933) Human Untagged Clone

CAT#: SC118954

DLST (untagged)-Human dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (DLST), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DLST
• Synonyms: DLTS; KGD2; PGL7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118954 sequence for NM_001933 edited (data generated by NextGen Sequencing)
  ATGCTGTCCCGATCCCGCTGTGTGTCTCGGGCGTTCAGCCGCTCGCTCTCCGCCTTCCAG
  AAGGGGAACTGCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTCCTTATGCCAGGGACCA
  GGTTACCCTAACAGCAGGAAGGTTGTCATTAACAACAGTGTCTTCAGTGTTCGCTTTTTC
  AGAACTACAGCTGTATGCAAGGATGACTTGGTTACAGTCAAAACCCCAGCGTTTGCAGAA
  TCTGTCACAGAGGGAGATGTCAGGTGGGAGAAAGCTGTTGGAGACACAGTTGCAGAAGAT
  GAAGTGGTTTGTGAGATTGAAACTGACAAGACATCTGTGCAGGTTCCATCACCAGCAAAT
  GGCGTGATTGAAGCTCTTTTGGTACCTGATGGGGGAAAAGTCGAAGGAGGCACTCCACTT
  TTCACACTCAGGAAAACTGGTGCTGCTCCTGCTAAGGCCAAGCCGGCTGAAGCTCCTGCT
  GCTGCAGCCCCAAAAGCAGAACCTACAGCAGCGGCAGTTCCTCCCCCTGCAGCACCCATA
  CCCACTCAGATGCCACCGGTGCCCTCGCCCTCACAACCTCCTTCTGGCAAACCTGTGTCT
  GCAGTAAAACCCACTGTTGCCCCACCACTAGCTGAGCCAGGAGCTGGCAAAGGTCTGCGT
  TCAGAACATCGGGAGAAAATGAACAGGATGCGGCAGCGCATTGCTCAGCGTCTGAAGGAG
  GCCCAGAATACATGTGCAATGCTGACAACTTTTAATGAGATTGACATGAGTAACATCCAG
  GAGATGAGGGCTCGGCACAAAGAGGCTTTTTTGAAGAAACATAACCTCAAACTAGGCTTC
  ATGTCGGCATTTGTGAAGGCCTCAGCCTTTGCCTTGCAGGAACAGCCTGTTGTAAATGCA
  GTGATTGACGACACAACCAAAGAGGTGGTGTATAGGGATTATATTGACATCAGTGTTGCA
  GTGGCCACCCCACGGGGTCTGGTGGTTCCAGTCATCAGGAATGTGGAAGCTATGAATTTT
  GCAGATATTGAACGGACCATCACTGAACTGGGAGAGAAGGCCCGAAAGAATGAACTTGCC
  ATTGAAGATATGGATGGTGGTACCTTCACCATTAGCAATGGAGGCGTTTTTGGCTCGCTC
  TTTGGAACACCCATTATCAACCCCCCTCAGTCTGCCATCCTGGGGATGCATGGCATCTTT
  GACAGGCCAGTGGCTATAGGAGGCAAGGTAGAGGTGCGGCCCATGATGTACGTGGCACTG
  ACCTATGATCACCGGCTGATTGATGGCAGAGAGGCTGTGACTTTCCTCCGCAAAATCAAG
  GCAGCGGTAGAGGATCCCAGAGTCCTCCTCCTGGATCTTTAG
  
  Clone variation with respect to NM_001933.4
  576 g=>a;1098 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001933 unedited
  AGTATTTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGCCCGCCCT
  CGGCTCCTCCGCCGTGATGCTGTCCCGATCCCGCTGTGTGTCTCGGGCGTTCAGCCGCTC
  GCTCTCCGCCTTCCAGAAGGGGAACTGCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTC
  CTTATGCCAGGGACCAGGTTACCCTAACAGCAGGAAGGTTGTCATTAACAACAGTGTCTT
  CAGTGTTCGCTTTTTCAGAACTACAGCTGTATGCAAGGATGACTTGGTTACAGTCAAAAC
  CCCAGCGTTTGCAGAATCTGTCACAGAGGGAGATGTCAGGTGGGAGAAAGCTGTTGGAGA
  CACAGTTGCAGAAGATGAAGTGGTTTGTGAGATTGAAACTGACAAGACATCTGTGCAGGT
  TCCATCACCAGCAAATGGCGTGATTGAAGCTCTTTTGGTACCTGATGGGGGAAAAGTCGA
  AGGAGGCACTCCACTTTTCACACTCAGGAAAACTGGTGCTGCTCCTGCTAAGGCCAAGCC
  GGCTGAAGCTCCTGCTGCTGCAGCCCCAAAAGCAGAACCTACAGCAGCGGCAGTTCCTCC
  CCCTGCAGCACCCATACCCACTCAGATGCCACCGGTGCCCTCGCCCTCACAACCTNCTTC
  TGGCAAACCTGTGTCTGCAGTAAAACCCACTGTTGCCCCACCACTAGCTGAGCCAGGAGC
  TGGCAAGGTCTGCGTTCAGACATCGGGAGAAAAGAACAGATGCGGCAGCGCATTGCTCAC
  CGTCTGAAGGAGGCCCAGATACATGTGCAATGCTGACACCTTTTATGAAGATGACATGAG
  TAACATCCAGAAGATGAGGGCTCGGCACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001933 unedited
  GCTATGCACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGAAATCT
  GCCCTTTAATTTGCATTTAAAATCAGTTTCTTTAAATACTTTAGTTACAAGTTCTGAATA
  GTGAAGACAGAGTAGTAGATGCTGCAGGCACTCAGTATTGGCTCCCACCCCTTCCCCCCT
  CCCCCACCCCCAGGCCTCACTTCTTGGCTTAAGATACAAAAGGGCACTCTCTACTGGCCC
  TGGTGTCAAACGTCAGCAGCCTCAGGCCTGGATGCAGTTGGATACAAAGCCATGAATCCC
  AGTGGCTCCAAGGAATTCCAATGATTGTGCCCAGATCCACGGGCAACACACCTTAGAAAT
  GACCTCATCCGTTTCACCACTAGGTACTAACACCCTCAGGGTGGTTTCCTACCCCAGTCC
  ACGTCCTCCAGATCTGTGTTCTGCACAGGAAGAGACAAGGCCCCGTTATAACGCACGAAG
  GAACAAACCCTGCCCCCTCATCCCCGCTGCTTCCCGCTACCTCAACTGCACACCCCAAGG
  CACTGCGACGCCACTCTAGACGGCACCCCCCCTCCCCCATTCCCCACCCCCCTTTTGCCC
  CCTTTCTGGACATCCTCTCGCCCCCCCGCCCTCATTCGCTCCACACCCCCCTTTCCATCA
  ATCGGCCCCACCGAGGCCCACCCTCTCTTTTGGGCTCTCTACGGCCCTTCCCCCCCCTCA
  CCGCCACCGCAGTCCCCTTGTTTATTCTTCGTTTCCCCCCCCATTATCCCCCCCTTCCCC
  TCCTCCCCACCTTTTCCCTCCCCCCACTCCTTTCCCCCCCGCCCCCATTTTTCTTCCCAC
  TATTCCCGCCCCCGTCCCCCCCTATTTTCCTCCCTTTTCCACGCCCCCCCTGCTCTTCAC
  TCTCTCCCTTCTTCTTCGTCCCTCCCCCCACGTTTATCCCCCCCACCTCCCTTTTCCCCC
  CCTCTCCTTCAGCCCTTCTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001933
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001933.3, NP_001924.2
• RefSeq Size: 2828 bp
• RefSeq ORF: 1362 bp
• Locus ID: 1743
• UniProt ID: P36957
• Cytogenetics: 14q24.3
• Domains: biotin_lipoyl, 2-oxoacid_dh
• Protein Pathways: Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways
• Gene Summary: This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).