EEF1B2 (NM_001959) Human Untagged Clone

CAT#: SC118903

EEF1B2 (untagged)-Human eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EEF1B2
• Synonyms: EEF1B; EEF1B1; EF1B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118903 sequence for NM_001959 edited (data generated by NextGen Sequencing)
  ATGGGTTTCGGAGACCTGAAAAGCCCTGCCGGCCTCCAGGTGCTCAACGATTACCTGGCG
  GACAAGAGCTACATCGAGGGGTATGTGCCATCACAAGCAGATGTGGCAGTATTTGAAGCC
  GTGTCCAGCCCACCGCCTGCCGACTTGTGTCATGCCCTACGTTGGTATAATCACATCAAG
  TCTTACGAAAAGGAAAAGGCCAGCCTGCCAGGAGTGAAGAAAGCTTTGGGCAAATATGGT
  CCTGCCGATGTGGAAGACACTACAGGAAGTGGAGCTACAGATAGTAAAGATGATGATGAC
  ATTGACCTCTTTGGATCTGATGATGAGGAGGAAAGTGAAGAAGCAAAGAGGCTAAGGGAA
  GAACGTCTTGCACAATATGAATCAAAGAAAGCCAAAAAACCTGCACTTGTTGCCAAGTCT
  TCCATCTTACTAGATGTGAAACCTTGGGATGATGAGACAGATATGGCGAAATTAGAGGAG
  TGCGTCAGAAGCATTCAAGCAGACGGCTTAGTCTGGGGCTCATCTAAACTAGTTCCAGTG
  GGATACGGAATTAAGAAACTTCAAATACAGTGTGTAGTTGAAGATGATAAAGTTGGAACA
  GATATGCTGGAGGAGCAGATCACTGCTTTTGAGGACTATGTGCAGTCCATGGATGTGGCT
  GCTTTCAACAAGATCTAA
  
  Clone variation with respect to NM_001959.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001959 unedited
  GTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTGGAGGTACGAACTTA
  AGACATGCCTATTTTATTAATTTACTTCCAAACGCAACGAAAGGTCCATGGACAATTTGT
  GGGCCATTTAATTCAGGGCCCCCAATTCGTACGTGGAGAAGTGGGAATGCAAAAGTACTT
  TGACCTTTAACCTTCGGTCCGGCGCGGTGGAGGGAAACGCCTCCGTCTCTATATAAGGAA
  TTTTCCGGTCTCTTCGGGTCCTTTTTCCTCTCTTCAGCGTGGGGCGCCCACAATTTGCGC
  GCTCTCTTTCTGCTGCTCCCCAGCTCTCGGATACAGCCGACACCATGGGTTTCGGAGACC
  TGAAAAGCCCTGCCGGCCTCCAGGTGCTCAACGATTACCTGGCGGACAAGAGCTACATCG
  AGGGGTATGTGCCATCACAAGCAGATGTGGCAGTATTTGAAGCCGTGTCCAGCCCACCGC
  CTGCCGACTTGTGTCATGCCCTACGTTGGTATAATCACATCAAGTCTTACGAAAAGGAAA
  AGGCCAGCCTGCCAGGAGTGAAGAAAGCTTTGGGCAAATATGGTCCTGCCGATGTGGAAG
  ACACTACAGGAAGTGGAGCTACAGATAGTAAAGATGATGATGACATTGACCTCTNTGGAT
  CTGATGATGAGGAGGAAAGTGAAGAAGCANAGAGGCTAAGGGAAGAACGTCTTGCACAAT
  ATGAATCANAGAAAGCCAAAAAACCTGCACTTGTTGCCAAGTCTTCCATCTTACTAGATG
  TGAAACCTTGGGATGATGAGACAGATATGGCGAAATAGAGGAGTGCGTCAGAAGCATTCA
  GCAGACGGCTAGTCTGGGGCTCATCTAACTAGTTCAGTGGGATACGAATTAGAAAACTCA
  ATACAGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001959 unedited
  CTTCCAGGGCCAGNANAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGGATCTGTTCA
  GGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATC
  TTTCAATCTTTTATTTAAATGCCATGATCCAGGATGGATTTTAGATCTTGTTGAAAGCAG
  CCACATCCATGGACTGCACATAGTCCTCAAAAGCAGTGATCTGCTCCTCCAGCATATCTG
  TTCCAACTTTATCATCTTCAACTACACACTGTATTTGAAGTTTCTTAATTCCGTATCCCA
  CTGGAACTAGTTTAGATGAGCCCCAGACTAAGCCGTCTGCTTGAATGCTTCTGACGCACT
  CCTCTAATTTCGCCATATCTGTCTCATCATCCCAAGGTTTCACATCTAGTAAGATGGAAG
  ACTTGGCAACAAGTGCAGGTTTTTTGGCTTTCTTTGATTCATATTGGGCAAGACGTTCTT
  CCCTTAGCCTCTTTGCTTCTTCACTTTCCTCCTCATCATCAGATCCAAAGAGGTCAATGG
  CATCATCATCTTTACTATCTGTAGCTCCACTTCCTGTAGTGTCTTCCACATCGGCAGGAC
  CATATTTGCCCAAAGCTTTCTTCACTCCTGGCAGGCTGGCCTTTTCCTTTTCGTAAGACT
  TGATGTGATTATACCAACGTANGGCATGACACAAAGTCGCAGGCGGTGGGCTGGACACNG
  CTTCAAATACTGCCACATCTGCTTGTGATGGCACATACCCCTCGATGTAGCTCTTGTCCG
  CCAGGTAATCGTTGAGCACCTGGNAGGCCGCAGGGCTTTTTCAGTCTCCGAAACCATGGT
  GTCNGCTGTATCCGGAGCTGGGGACAGANAAAGAACGCGCAATTGGGGCCGCCCACGCTG
  AGGAGAAAAAGACCNAAAGACGNNAAATTCTATTAAGACCGAGCGGTTCCTCACCGGCGG
  ACGAAGTAAGGCAAAACTTTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001959
• Insert Size: 1070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001959.2, NP_001950.1
• RefSeq Size: 961 bp
• RefSeq ORF: 678 bp
• Locus ID: 1933
• UniProt ID: P24534
• Cytogenetics: 2q33.3
• Domains: EF1BD
• Gene Summary: This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq, Jul 2008]
  Transcript Variant: This predominant variant (1) has a distinct 5' UTR. Variants 1, 2 and 3 encode the same protein.