MC1 Receptor (MC1R) (NM_002386) Human Untagged Clone

CAT#: SC118656

MC1R (untagged)-Human melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) (MC1R)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MC1 Receptor
• Synonyms: CMM5; MSH-R; SHEP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002386 edited
  ATGGCTGTGCAGGGATCCCAGAGAAGACTTCTGGGCTCCCTCAACTCCACCCCCACAGCC
  ATCCCCCAGCTGGGGCTGGCTGCCAACCAGACAGGAGCCCGGTGCCTGGAGGTGTCCATC
  TCTGACGGGCTCTTCCTCAGCCTGGGGCTGGTGAGCTTGGTGGAGAACGCGCTGGTGGTG
  GCCACCATCGCCAAGAACCGGAACCTGCACTCACCCATGTACTGCTTCATCTGCTGCCTG
  GCCTTGTCGGACCTGCTGGTGAGCGGGAGCAACGTGCTGGAGACGGCCGTCATCCTCCTG
  CTGGAGGCCGGTGCACTGGTGGCCCGGGCTGCGGTGCTGCAGCAGCTGGACAATGTCATT
  GACGTGATCACCTGCAGCTCCATGCTGTCCAGCCTCTGCTTCCTGGGCGCCATCGCCGTG
  GACCGCTACATCTCCATCTTCTACGCACTGCGCTACCACAGCATCGTGACCCTGCCGCGG
  GCGCGGCAAGCCGTTGCGGCCATCTGGGTGGCCAGTGTCGTCTTCAGCACGCTCTTCATC
  GCCTACTACGACCACGTGGCCGTCCTGCTGTGCCTCGTGGTCTTCTTCCTGGCTATGCTG
  GTGCTCATGGCCGTGCTGTACGTCCACATGCTGGCCCGGGCCTGCCAGCACGCCCAGGGC
  ATCGCCCGGCTCCACAAGAGGCAGCGCCCGGTCCACCAGGGCTTTGGCCTTAAAGGCGCT
  GTCACCCTCACCATCCTGCTGGGCATTTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCAT
  CTCACACTCATCGTCCTCTGCCCCGAGCACCCCACGTGCGGCTGCATCTTCAAGAACTTC
  AACCTCTTTCTCGCCCTCATCATCTGCAATGCCATCATCGACCCCCTCATCTACGCCTTC
  CACAGCCAGGAGCTCCGCAGGACGCTCAAGGAGGTGCTGACATGCTCCTGGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002386 unedited
  CCCCCCCCGTTTAGAATTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACGAGGGC
  AGGGAGGGAGCTGAGGACCAGGCTTGGTTGTGAGAATCCCTGAGCCCAGGCGGTTGATGC
  CAGGAGGTGTCTGGACTGGCTGGGCCATGCCTGGGCTGACCTGTCCAGCCAGGGAGAGGG
  TGTGAGGGCAGATCTGGGGGTGCCCAGATGGAAGGAGGCAGGCATGGGGGACACCCAAGG
  CCCCCTGGCAGCACCATGAACTAAGCAGGACACCTGGAGGGGAAGAACTGTGGGGACCTG
  GAGGCCTCCAACGACTCCTTCCTGCTTCCTGGACAGGACTATGGCTGTGCAGGGATCCCA
  GAGAAGACTTCTGGGCTCCCTCAACTCCACCCCCACAGCCATCCCCCAGCTGGGGCTGGC
  TGCCAACCAGACAGGAGCCCGGTGCCTGGAGGTGTCCATCTCTGACGGGCTCTTCCTCAG
  CCTGGGGCTGGTGAGCTTGGTGGAGAACGCGCTGGTGGTGGCCACCATCGCCAAGAACCG
  GAACCTGCACTCACCCATGTACTGCTTCATCTGCTGCCTGGCCTTGTCGGACCTGCTGGT
  GAGCGGGAGCAACGTGCTGGAGACGGCCGTCATCCTCCTGCTGGAGGCCGGTGCACTGGT
  GGCCCGGGCTGCGGTGCTGCAGCAGCTGGACAATGTCATTGACGTGATCACCTGCAGCTC
  CATGCTGTCCAGCCTCTGCTTCCTGGGCGCCATCGCCGTGGACCGCTACATCTNCATCTT
  CTACGCACTGCGCTACCACAGCATCGTGACCCTGCCGCGGNCGCTGCAAGCCGTTGCTGC
  CATCATGGTGGCCAGTGTCGTCTTCAGCACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002386 unedited
  CCCCGAAATTGTGTGNACCGCGGNNCCGCATTTTACGATCGAGTTTTTTTTTTTTTTTTT
  TTTTTTTTTTGGGGGATGCTGAGTCACATTTATTACCAGACTTTCCTGGCCCCATGCTCA
  CAGGCACTGGTCACTGAGTCAGGCATTTGCCAGGGCTGTCTGCTTGGGCGACTGCTGCAG
  GAAAGCAGGCTGAGGCCCCAGTGCCCAGTCTGAGCCTTAGAACCGGCCCTTAGGAGGGCT
  CAGCCCCATACCACTAGGGGGGCTCCTGCAAACCTGGTCCCTTGGCCTGAGGCAGGGATT
  TCACCTCCTTTTGCAGGAGCCGCTGCAAGGCTGTGGATGAAGCTTTCTGGTCACAAAGCC
  CCTTCTCCTGGGGCTGCTGTGGGAGTAGCTCTTGGCACACACTTCCACCCAGAGACTTCA
  CATCCCAGCTGACGAGGGCACGGTCCTGAGAGCTGGCAGCAAAGATGGGGTGGGGTGGAT
  GCTGCTCAGTCATTGGAGCAGACGGAGTGTCCCAGGAGTGGGCGGAAAATGTCTCTTTAG
  GAGCCTGAGGTTGGCCCCTGGTGCCCGGAGCCTGAGGCCCCACAACCTCCCCATGTCCTC
  CTCCGTTCCCCACGACTGCTGGTGAGAAGTCCTGCCTGGAGTTTTGCAGGGACCCCTCCC
  TGGACCCAGAAGGGTCCGCGCTTCAACACTTTCAGAGATCATTTAGTCCATCCTCTTTGA
  CAAACGGGGACCAGGGAGGTAAGGAACTGCCCTAGGTCTCACAGGAACCAGACCCACAAT
  ATTACCACCTTCCTCTGCCCAGCACACTTAAAGCCGCGTGCACCGCGCTCACCAGGAGCA
  TGTCAGCACCTTCTTTGACGTCCTTGCGNACTCCTGGGCTGTGAAGGCGTACATGAGGGG
  GTCCATGATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002386
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002386.2, NP_002377.3
• RefSeq Size: 2400 bp
• RefSeq ORF: 954 bp
• Locus ID: 4157
• UniProt ID: Q01726
• Cytogenetics: 16q24.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Melanogenesis, Neuroactive ligand-receptor interaction
• Gene Summary: This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]