P2X1 (P2RX1) (NM_002558) Human Untagged Clone

CAT#: SC118594

P2RX1 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P2X1
• Synonyms: P2X1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118594 sequence for NM_002558 edited (data generated by NextGen Sequencing)
  ATGGCACGGCGGTTCCAGGAGGAGCTGGCCGCCTTCCTCTTCGAGTATGACACCCCCCGC
  ATGGTGCTGGTGCGTAATAAGAAGGTGGGCGTTATCTTCCGACTGATCCAGCTGGTGGTC
  CTGGTCTACGTCATCGGGTGGGTGTTTCTCTATGAGAAGGGCTACCAGACCTCGAGCGGC
  CTCATCAGCAGTGTCTCTGTGAAACTCAAGGGCCTGGCCGTGACCCAGCTCCCTGGCCTC
  GGCCCCCAGGTCTGGGATGTGGCTGACTACGTCTTCCCAGCCCAGGGGGACAACTCCTTC
  GTGGTCATGACCAATTTCATCGTGACCCCGAAGCAGACTCAAGGCTACTGCGCAGAGCAC
  CCAGAAGGGGGCATATGCAAGGAAGACAGTGGCTGTACCCCTGGGAAGGCCAAGAGGAAG
  GCCCAAGGCATCCGCACGGGCAAGTGTGTGGCCTTCAACGACACTGTGAAGACGTGTGAG
  ATCTTTGGCTGGTGCCCCGTGGAGGTGGATGACGACATCCCGCGCCCTGCCCTTCTCCGA
  GAGGCCGAGAACTTCACTCTTTTCATCAAGAACAGCATCAGCTTTCCACGCTTCAAGGTC
  AACAGGCGCAACCTGGTGGAGGAGGTGAATGCTGCCCACATGAAGACCTGCCTCTTTCAC
  AAGACCCTGCACCCCCTGTGCCCAGTCTTCCAGCTTGGCTACGTGGTGCAAGAGTCAGGC
  CAGAACTTCAGCACCCTGGCTGAGAAGGGTGGAGTGGTTGGCATCACCATCGACTGGCAC
  TGTGACCTGGACTGGCACGTACGGCACTGCAGACCCATCTATGAGTTCCATGGGCTGTAC
  GAAGAGAAAAATCTCTCCCCAGGCTTCAACTTCAGGTTTGCCAGGCACTTTGTGGAGAAC
  GGGACCAACTACCGTCACCTCTTCAAGGTGTTTGGGATTCGCTTTGACATCCTGGTGGAC
  GGCAAGGCCGGGAAGTTTGACATCATCCCTACAATGACCACCATCGGCTCTGGAATTGGC
  ATCTTTGGGGTGGCCACAGTTCTCTGTGACCTGCTGCTGCTTCACATCCTGCCTAAGAGG
  CACTACTACAAGCAGAAGAAGTTCAAATACGCTGAGGACATGGGGCCAGGGGCGGCTGAG
  CGTGACCTCGCAGCTACCAGCTCCACCCTGGGCCTGCAGGAGAACATGAGGACATCCTGA
  
  Clone variation with respect to NM_002558.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002558 unedited
  CAGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCCAAGAGC
  TGCTCTGATCACCCAGGGATTCTCTCTCCAACCCAAGTGCCTCCAGCTGACCTCTGGCTC
  CTGTCCTCTGGCTCCACCTGCACCGCCCTGCTCTTCCTAAGGGGCCAGGAAGCCCCCAGA
  AGCTCTACCATCGACGTGGGTGGTGGCACCCGGCTCACCCTGAGAGCAGAGGCCGTGCAG
  GGGGCTCAGTTCTGAGCCCCAGCCGGCCCACCATGGCACGGCGGTTCCAGGAGGAGCTGG
  CCGCCTTCCTCTTCGAGTATGACACCCCCCGCATGGTGCTGGTGCGTAATAAGAAGGTGG
  GCGTTATCTTCCGACTGATCCAGCTGGTGGTCCTGGTCTACGTCATCGGGTGGGGTGTTT
  CTCTATGAGAAGGGCTACCAGACCTCGAGCGGCCTCATCAGCAGTGTCTCTGTGAAACTC
  AAGGGGCCTGGCCGTGACCCAGCTCCCTGGCCTCGGCCCCCANGTCTGGGGATGGTGGCT
  GACTACGTCTTCCCAGCCCAGGGGGACAACTCCTNTCGTGGTCATGACCAATTTCATCGT
  GACCCCGAAGCAGACTCAAGGCTACTGCGCAGAGCACCCCANAAGGGGGCATATGCAAGG
  AAGACAGTGGCTGTACCCCTGNGAAGGCCAGNAGGAAGGCCCAAGGCATCCGCCGGGCAA
  GTTGTGTGGCCTTCACGACACTGTGGAGACGTGTGAGATCTTTGGCTGTGCCCCGTNGNA
  GGTGGATGACGACATCCCGCGCCCTGCCCTTCTCCGAGAGGCCGAGAAACTCACTCTTTT
  CATCAAGACAGCATCAGCTTTCCACGCTTCAAGTCAACAGCGCACCTGGGGAAGGAGGGG
  AATGCTGGCCAATGAAGACTGCCTTTTTACAAGACCTGCACCCCTGTGCCAGCCTTCACT
  TGGCTACGTGGGCCAGAGTCAGCCAAACTCACACCTGTCGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002558 unedited
  CGGGGGGGAACAGACCAGACNTCCTTCCNNNCNCCGTCAATCGTAGNACCNCGGNNCCGC
  ATTTTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTGGGCCCAATCCCTTTATTGGACCCCACCCCTCGGTTTCCCCAAAACTTTTGTTT
  AAAAAAAACCAGGCAGGCCTTGGGAGCTGGGGGTGAACCACAGGCAGGGGGCCCAAGATT
  TGGGTGGGGGTCCCACCCCAACCCCAGGGAGCTGAGTAAGGGTTTATGGGTGGGAGGGGT
  GTCCTGGCCTGGCACACTTGGAAAAAAACCTTTTCCTTTGGGGGGGACATGGGCCCGACC
  ATGTGGGGGGGCTTGGGGGGGGAAAAGTGGGTAAAAGGGTGGGTTTGCAGGGCCCAAACT
  GTTCACATACACAATGGGGAAGGAAGCCTGGTGCATTTAAGGGGGGGGTACAACGGGGTG
  GGGGCATGAAAAACTGGGGGGGACGTGAGCACCCAAAACACATGCTGAAGGGGGTTGAGA
  ACCTGTTCAATGGTTGTCCCTTAACAGGAAATGGCGGGCTCAGGGTTAATCTGACCCCCC
  CTTAAGGGCCTTAAATCAAAAAGTTCGAGGACGGCCCTTTTTAAAATTAAGCCCCCAAAG
  GCCCGGGGGGGTTGGCCATTCACCACAAAAAAACGGGCACCCCTTGGAACTTTTGGGTTA
  GGAAGGCTGGGGGGGGGGCCCCACGAGACTTGCGATTTGCCAAAAAATTTCCCCCAGGGG
  AAACCCAAGGGGCACGGAAAAAACAAGGGCTTTAAAACAGGGCGTGGTTTAATAAAAAAA
  CTTTTTTTGTAAAAAGAGAAAAAGCNCCCCAGACTCGTTCACCATGTGGGGCCCACTCAC
  CCCCCCCTTTATTTCTCGGCCCTTGAAAATGGGGTTTTTCTCCCCCGAGGAAAAGAAATT
  TGGGGGTCCCTTTTTATTTTATTTCACGTTAAATTTAACCCCCCTGGGGTTATGCGCGGC
  CCCACGCGGAG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002558
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002558.2, NP_002549.1
• RefSeq Size: 2910 bp
• RefSeq ORF: 1200 bp
• Locus ID: 5023
• UniProt ID: P51575
• Cytogenetics: 17p13.2
• Domains: P2X_receptor
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene belongs to the P2X family of G-protein-coupled receptors. These proteins can form homo-and heterotimers and function as ATP-gated ion channels and mediate rapid and selective permeability to cations. This protein is primarily localized to smooth muscle where binds ATP and mediates synaptic transmission between neurons and from neurons to smooth muscle and may being responsible for sympathetic vasoconstriction in small arteries, arterioles and vas deferens. Mouse studies suggest that this receptor is essential for normal male reproductive function. This protein may also be involved in promoting apoptosis. [provided by RefSeq, Jun 2013]