PSMA2 (NM_002787) Human Untagged Clone

CAT#: SC118410

PSMA2 (untagged)-Human proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMA2
• Synonyms: HC3; MU; PMSA2; PSC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118410 sequence for NM_002787 edited (data generated by NextGen Sequencing)
  ATGGCGGAGCGCGGGTACAGCTTTTCGCTGACTACATTCAGCCCGTCTGGTAAACTTGTC
  CAGATTGAATATGCTTTGGCTGCTGTAGCTGGAGGAGCCCCGTCCGTGGGAATTAAAGCT
  GCAAATGGTGTGGTATTAGCAACTGAGAAAAAACAGAAATCCATTCTGTATGATGAGCGA
  AGTGTACACAAAGTAGAACCAATTACCAAGCATATAGGTTTGGTGTACAGTGGCATGGGC
  CCCGATTACAGAGTGCTTGTGCACAGAGCTCGAAAACTAGCTCAACAATACTATCTTGTG
  TACCAAGAACCCATTCCTACAGCTCAGCTGGTACAGAGAGTAGCTTCTGTGATGCAAGAA
  TATACTCAGTCAGGTGGTGTTCGTCCATTTGGAGTTTCTTTACTTATTTGTGGTTGGAAT
  GAGGGACGACCATATTTATTTCAGTCAGATCCATCTGGAGCTTACTTTGCCTGGAAAGCT
  ACAGCAATGGGAAAGAACTATGTGAATGGGAAGACTTTCCTTGAGAAAAGATATAATGAA
  GATCTGGAACTTGAAGATGCCATTCATACAGCCATCTTAACCCTAAAGGAAAGCTTTGAA
  GGGCAAATGACAGAGGATAACATAGAAGTTGGAATCTGCAATGAAGCTGGATTTAGGAGG
  CTTACTCCAACTGAAGTTAAGGATTACTTGGCTGCCATAGCATAA
  
  Clone variation with respect to NM_002787.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002787 unedited
  CATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGCTTTGGCTCTTC
  GGGTAAAGATGGCGGAGCGCGGGTACAGCTTTTCGCTGACTACATTCAGCCCGTCTGGTA
  AACTTGTCCAGATTGAATATGCTTTGGCTGCTGTAGCTGGAGGAGCCCCGTCCGTGGGAA
  TTAAAGCTGCAAATGGTGTGGTATTAGCAACTGAGAAAAAACAGAAATCCATTCTGTATG
  ATGAGCGAAGTGTACACAAAGTAGAACCAATTACCAAGCATATAGGTTTGGTGTACAGTG
  GCATGGGCCCCGATTACAGAGTGCTTGTGCACAGAGCTCGAAAACTAGCTCAACAATACT
  ATCTTGTGTACCAAGAACCCATTCCTACAGCTCAGCTGGTACAGAGAGTAGCTTCTGTGA
  TGCAAGAATATACTCAGTCAGGTGGTGTTCGTCCATTTGGAGTTTCTTTACTTATTTGTG
  GTTGGAATGAGGGACGACCATATTTATTTCAGTCAGATCCATCTGGAGCTTACTTTGCCT
  GGAAAGCTACAGCAATGGGAAAGAACTATGTGAATGGGAAGACTTTCCTTGAGAAAAGAT
  ATAATGAAGATCTGGAACTTGAAGATGCCATTCATACAGCCATCTTAACCCTAAAGGAAA
  GCTTTGAAGGGCAAATGACAGAGGATAACATAGAAGTTGGAATCTGCAATGAAGCTGGAT
  TTANGANGCTTACTCCAACTGAAANTAAGGATTACTTGGCTGCCATAGCATAACAATGAA
  GTGACTGNAAAATCCAGAAATTCAGATAATCTATCTACTNTAACATNGTTAAAGTATNGT
  TTTGTTTTGCAGACTTTTTGCATACTTTATTCTACATGGNTTAAATCGACTGTTNTTAAA
  TGACACTATNAATCCCTATAACTGTTAACCACCTTCAGCCTTTAGAGTGCTAAAATTTAA
  CAGTATTCCTGCTTTNTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002787 unedited
  ATTAGCATTTTATTTGAAGCACTTATGCCTCATCCTTGATTTAAAAAGTCTAACAAAATC
  TAAGAACCCGGGTTCCTTTAAACATGTTTACCTACTTGTGGAGACACCAACAGGAACCAG
  CAAACCATTGTTTATTTATTTACATAATGTATCTCCCATGGAGACCTATTTGGCTTACCA
  CTCTTCATAGGCCTAGTACAATCTCATAGAGCACCCTAGGATTTCACTGATCAATACCTA
  ATGGCACCTAAAAGTACCTATGCTTCTTTTCTTGTTCCATGAATCCGTGAAAGTCTGCAG
  ATCCTCCCAGCTCTAGAAGCAGTCATGTTTTTTACCAAAATCAGCATTTTGCTTTATGGA
  AACACAGGCAACATCTGATAGCGAAGAGGGCATTAAGATTTGACTTAACCAAAATTGCCT
  GACAACGAAAAAGTCATTTCATCATTCTGCTTGGCAATGTACTCTTCAGAAATCAACTGT
  GATAAAAAGCAGGAAATAACTGTTAAAATTTTAGCAACTCCTAAAAGGCTGGAAGGTGGG
  TTTAACAGTTTATTANGATTTATAAGTGTCATTTTAAAAACAGTCGATTTAAACCATGTT
  AGAATAAGTATGCAAAAAGTCTGCAAAACANAACATACTTTAAACATGTTTAAGTAGATA
  GATTATCTGAAACTCCTGATTTTTCAGTCACCTCACTGCTATGCTATGGCAGCCAAGTAC
  ATCCTCACCTCCAGTTGGGATCAGTCCCCCTAATCCCACCTCNTTTGATAATCCCAACCC
  CTATGCTATCCTCTCGCTATTGCCCTTCAAAGCTCTCTTTTAGGCTAACAAGGCCGTTTG
  AATTGCATCTTCAATTCCCAATCTCCATTCTTCTTTCTAACCGCAGCGCTCTCATTCCAT
  ATACCTTCCCATGGTGCAATTTTCAGCCAACAACCCCACATGTTCTCCTTGAAAAATTGC
  GCCCCCTCTCCACCACAATATTCACAACCTCAATGATATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002787
• Insert Size: 1610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002787.3, NP_002778.1
• RefSeq Size: 1477 bp
• RefSeq ORF: 705 bp
• Locus ID: 5683
• UniProt ID: P25787
• Cytogenetics: 7p14.1
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq, Jul 2008]