RFC2 (NM_002914) Human Untagged Clone

CAT#: SC118345

RFC2 (untagged)-Human replication factor C (activator 1) 2, 40kDa (RFC2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFC2
• Synonyms: RFC40
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118345 sequence for NM_002914 edited (data generated by NextGen Sequencing)
  ATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGTGGAGGCCCAGGACTCTGACCCT
  GCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCACTACGAACTGCCGTGGGTTGAA
  AAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAATGAAGACACCGTGAGCAGGCTA
  GAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCATCATTGCGGGCCCTCCAGGAACC
  GGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCTGCTGGGCCCAGCACTCAAAGAT
  GCCATGTTGGAACTCAATGCTTCAAATGACAGCATGACCGACGGAGCCCAGCAAGCCTTG
  AGGAGAACCATGGAAATCTACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGCTTCG
  GATAAGATCATCGAGCCCATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCTGACC
  GACGCCCAGATCCTCACCAGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTACACT
  GATGACGGCCTAGAAGCCATCATCTTCACGGCCCAGGGAGACATGAGGCAGGCGCTGAAC
  AACCTGCAGTCCACCTTCTCAGGATTTGGCTTCATTAACAGTGAGAACGTGTTCAAGGTC
  TGTGACGAGCCCCACCCACTGCTGGTAAAGGAGATGATCCAGCACTGTGTGAATGCCAAC
  ATTGACGAAGCCTACAAGATTCTTGCTCACTTGTGGCATCTGGGCTACTCACCAGAAGAT
  ATCATTGGCAACATCTTTCGAGTGTGTAAAACTTTCCAAATGGCAGAATACCTGAAACTG
  GAGTTTATCAAGGAAATTGGATACACTCACATGAAAATAGCGGAAGGAGTGAACTCTCTT
  TTGCAGATGGCAGGCCTCCTGGCAAGGCTGTGTCAGAAGACAATGGCCCCGGTGGCCAGT
  TAG
  
  Clone variation with respect to NM_002914.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002914 unedited
  NNGGTCGGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCTCGGGT
  GGCGGGAAGAGGAGGCGCGAGAATGGAGGTGGAGGCCGTCTGTGGTGGCGCGGGCGAGGT
  GGAGGCCCAGGACTCTGACCCTGCCCCTGCCTTCAGCAAGGCCCCCGGCAGCGCCGGCCA
  CTACGAACTGCCGTGGGTTGAAAAATATAGGCCAGTAAAGCTGAATGAAATTGTCGGGAA
  TGAAGACACCGTGAGCAGGCTAGAGGTCTTTGCAAGGGAAGGAAATGTGCCCAACATCAT
  CATTGCGGGCCCTCCAGGAACCGGCAAGACCACAAGCATTCTGTGCTTGGCCCGGGCCCT
  GCTGGGCCCAGCACTCAAAGATGCCATGTTGGAACTCAATGCTTCAAATGACAGGGGCAT
  TGACGTTGTGAGGAATAAAATTAAAATGTTTGCTCAACAAAAAGTCACTCTTCCCAAAGG
  CCGACATAAGATCATCATTCTGGATGAAGCAGACAGCATGACCGACGGAGCCCAGCAAGC
  CTTGAGGAGAACCATGGAAATCTACTCTAAAACCACTCGCTTCGCCCTTGCTTGTAATGC
  TTCGGATAAGATCATCGAGCCCATTCAGTCCCGCTGTGCAGTCCTCCGGTACACAAAGCT
  GACCGACGCCCAGATCCTCACCAGGCTGATGAATGTTATCGAGAAGGAGAGGGTACCCTA
  CACTGATGACGGCCTAGAAGCCATCATCTTCACGGCCCAGGGAGACATGANGCAGGCGCT
  GAACAACCTGCAGTCCACCTTCTCAGGATTTGGCTTCATTAACAGTGAGAACGTGNTCAA
  GGTCTGTGACGAGCCCCACCCACTGCTGGGTAAGGNAGAGATCCAGCACTGTGTGAATGC
  CACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002914 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAAGTCACGGGAGT
  TTATTTATTTAATTTTTTTCCCCAGATGGAGACTCTGTCGCCCAGGCTGGAGTGCAATGG
  TGTGATCTTGGCTCACTGCAACCTCCACCTCCCGGGCTCAAGCGATTCTCCCGCCACAGC
  CTCCCGAGTAGCTGGGATTACAGGTGCCCGCCACCACACCCAGCTAATTTTTATATTTTT
  AGTAAAGACAGGGTTTCCCCATGTTGGCCAGGCTGGTCTTGAACTTCTGACCTCAGGTGA
  TCCACCCGCCTCGGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCTACCGTGCCCGGCC
  AGCCACTGGAGTTTAAAGGACAGTCATGTTGGCTCCAGCCTAAGGCGGCATTTTCCCCCA
  TCAGAAAGCCGCGGCTCCTGTACCTCAGAATAGGGCACCTGTAAGTCAGTCAGTGAAGTC
  TCTGCTCTAACTGGCCACCGGGGCCATTGTCTTCTGACACAGCCTTGCCAGGAGGCCTGC
  CATCTGCCAAAGAGAGTTCACTCCTTCCGCTATTTTCATGTGAGTGTATCCAATTTCCCT
  TGATAACTCCAGTTTCAGGTATTCTGCCATTTGGAAAGTTTTACACACCTCGAAGATGTT
  GCCAATGATATCTTCTGGTGAGTAGCCCAGATGCCACAAGTGAGCACGAATCTTGTAGGC
  TTCGTCAATGGTGCCATTCACACAGTGGCTGGATCATCTCCTTTACCAGCAGTGGGTGGG
  GCTCGTCACAAGACCTTGAACACGTTTTCACTGGTAATGAAGCCAAATCCTGAAAAGGTG
  GACCTGCACGTTGTCCACCGCCTGTCTCATGTCTTCCTGGGCCGCGAAAATGAAGGCGTT
  CTAAGCCGTCATCAGTGTAAGGACCCCTCTTCTTTCTGGAAACATTCAATAAGCTGGGGG
  AGGAACTGGCCGTCNGCCCACNTTTGGTACCCGGAGACTGTCCAANGGCACTGAATGGCC
  TCCAGATCTTATCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002914
• Insert Size: 1630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002914.3, NP_002905.2
• RefSeq Size: 1613 bp
• RefSeq ORF: 963 bp
• Locus ID: 5982
• UniProt ID: P35250
• Cytogenetics: 7q11.23
• Domains: AAA, AAA
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.