Syndecan 1 (SDC1) (NM_002997) Human Untagged Clone

CAT#: SC118272

SDC1 (untagged)-Human syndecan 1 (SDC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Syndecan 1
• Synonyms: CD138; SDC; SYND1; syndecan
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002997 edited
  GAATTCGGCACGAGGCCGAATCCGAGCCGAGCGGAGAGGAATCCGGCAGTAGAGAGCGGA
  CTCCAGCCGGCGGACCCTGCAGCCCTCGCCTGGGACAGCGGCGCGCTGGGCAGGCGCCCA
  AGAGAGCATCGAGCAGCGGAACCCGCGAAGCCGGCCCGCAGCCGCGACCCGCGCAGCCTG
  CCGCTCTCCCGCCGCCGGTCCGGGCAGCATGAGGCGCGCGGCGCTCTGGCTCTGGCTGTG
  CGCGCTGGCGCTGAGCCTGCAGCCGGCCCTGCCGCAAATTGTGGCTACTAATTTGCCCCC
  TGAAGATCAAGATGGCTCTGGGGATGACTCTGACAACTTCTCCGGCTCAGGTGCAGGTGC
  TTTGCAAGATATCACCTTGTCACAGCAGACCCCCTCCACTTGGAAGGACACGCAGCTCCT
  GACGGCTATTCCCACGTCTCCAGAACCCACCGGCCTGGAGGCTACAGCTGCCTCCACCTC
  CACCCTGCCGGCTGGAGAGGGGCCCAAGGAGGGAGAGGCTGTAGTCCTGCCAGAAGTGGA
  GCCTGGCCTCACCGCCCGGGAGCAGGAGGCCACCCCCCGACCCAGGGAGACCACACAGCT
  CCCGACCACTCATCAGGCCTCAACGACCACAGCCACCACGGCCCAGGAGCCCGCCACCTC
  CCACCCCCACAGGGACATGCAGCCTGGCCACCATGAGACCTCAACCCCTGCAGGACCCAG
  CCAAGCTGACCTTCACACTCCCCACACAGAGGATGGAGGTCCTTCTGCCACCGAGAGGGC
  TGCTGAGGATGGAGCCTCCAGTCAGCTCCCAGCAGCAGAGGGCTCTGGGGAGCAGGACTT
  CACCTTTGAAACCTCGGGGGAGAATACGGCTGTAGTGGCCGTGGAGCCTGACCGCCGGAA
  CCAGTCCCCAGTGGATCAGGGGGCCACGGGGGCCTCACAGGGCCTCCTGGACAGGAAAGA
  GGTGCTGGGAGGGGTCATTGCCGGAGGCCTCGTGGGGCTCATCTTTGCTGTGTGCCTGGT
  GGGTTTCATGCTGTACCGCATGAAGAAGAAGGACGAAGGCAGCTACTCCTTGGAGGAGCC
  GAAACAAGCCAACGGCGGGGCCTACCAGAAGCCCACCAAACAGGAGGAATTCTATGCCTG
  ACGCGGGAGCCATGCGCCCCCTCCGCCCTGCCACTCACTAGGCCCCCACTTGCCTCTTCC
  TTGAAGAACTGCAGGCCCTGGCCTCCCCTGCCACCAGGCCACCTCCCCAGCATTCCAGCC
  CCTCTGGTCGCTCCTGCCCACGGAGTCGTGGGGTGTGCTGGGAGCTCCACTCTGCTTCTC
  TGACTTCTGCCTGGAGACTTAGGGCACCAGGGGTTTCTCGCATAGGACCTTTCCACCACA
  GCCAGCACCTGGCATCGCACCATTCTGACTCGGTTTCTCCAAACTGAAGCAGCCTCTCCC
  CAGGTCCAGCTCTGGAGGGGAGGGGGATCCGACTGCTTTGGACCTAAATGGCCTCATGTG
  GCTGGAAGATCCTGCGGGTGGGGCTTGGGGCTCACACACCTGTAGCACTTACTGGTAGGA
  CCAAGCATCTTGGGGGGGTGGCCGCTGAGTGGCAGGGGACAGGAGTCCACTTTGTTTCGT
  GGGGAGGTCTAATCTAGATATCGACTTGTTTTTGCACATGTTTCCTCTAGTTCTTTGTTC
  ATAGCCCAGTAGACCTTGTTACTTCTGAGGTAAGTTAAGTAAGTTGATTCGGTATCCCCC
  CATCTTGCTTCCCTAATCTATGGTCGGGAGACAGCATCAGGGTTAAGAAGACTTTTTTTT
  TTTTTTTTXXXXXXXXXXXXXXXXXXXXXAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002997 unedited
  CGGGTAGCATTTGTATACGACTTATATAGGGCGGCCGCGAATTCGCACGAGGCCGAATCC
  GAGCCGAGCGGAGAGGAATCCGGCAGTAGAGAGCGGACTCCAGCCGGCGGACCCTGCAGC
  CCTCGCCTGGGACAGCGGCGCGCTGGGCAGGCGCCCAAGAGAGCATCGAGCAGCGGAACC
  CGCGAAGCCGGCCCGCAGCCGCGACCCGCGCATCCTGCCGCTCTCCCGCCGCCGGTCCGG
  GCAGCATGAGGCGCGCGGCGCTCTGGCTCTGGCTGTGCGCGCTGGCGCTGAGCCTGCAGC
  CGGCCCTGCCGCAAATTGTGGCTACTAATTTGCCCCCTGAAGATCAAGATGGCTCTGGGG
  ATGACTCTGACAACTTCTCCGGCTCAGGTGCAGGTGCTTTGCAAGATATCACCTTGTCAC
  AGCAGACCCCCTCCACTTGGAAGGACACGCAGCTCCTGACGGCTATTCCCACGTCTCCAG
  AACCCACCGGCCTGGAGGCTACAGCTGCCTCCACCTCCACCCTGCCGGCTGGAGAGGGGC
  CCAAGGAGGGAGAGGCTGTAGTCCTGCCAGAAGTGGAGCCTGGCCTCACCGCCCGGGAGC
  AGGAGGCCACCCCCCGACCCAGGGAGACCACACAGCTCCCGACCACTCATCAGGCCTCAA
  CGACCACAGCCACCACGGCCCAGGAGCCCGCCACCTCCCACCCNCACAGGGACATGCAGC
  CTGGCCACCATGAGACCTCAACCCCTGCAGGACCCAGCCAAGCTGACCTTCACACTCCCC
  ACACAGAGGATGGGAGTCCTTCTGCCANCGAGAGGGCTGCTNGAGATGGAGCCTNCAGTC
  AGCTCCCAGCACAGAGGGCTCTNGGGGAGCAGACTTTCACTTTGAACCTCCGGGGAGAAT
  ACGCTGTATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002997 unedited
  CTCCCCCNAATTGACTTGNACCGCGCCCGTATNCTAGNGATCNGTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC
  CCGGGCCCCCTTTTTTTAAAAGTTTATTCCTTTGGGAAAACCTCCGGGCCGGGGAAAAAC
  CCCTTTGGATAAAATAAAGTTTTCCCAAAACCAAAAAACTTTTTCTTTTTGGAAAATTTT
  CCTGATCCCCCCCCAGGGCCCAGGGGGGGCCCCCGGGGCCTACCGGAAAACCTGCCCTTG
  GATGGGGGCCCTAAAAACCAGCTTTGCCCAAAAACCCCCCTCGTTAATTTCCCGGGGGAA
  ACCCCCCCCGGGGAACGGCCCCCCCGGCCAAGAAGGGGGGGTTTTTTCAAGAAAAATTTT
  TTAGGGCCCCGGGGGCGCCCCCCCAAAAAGGTCTCTTTTGTGGGGGGGGGGGGGGGGCCC
  CCCATTTTTTTTTTTTTCCCCGGGGGGTGTTTTCTCCAAGGGGGGGGGGCGGCCCCCCCC
  CCCCCAAAGAGAGGGGGTCGGCGGGGGGGCGGGCCCCCCCCCCCTTTTTTTTTTTATAAG
  AAGGGGGGCCCCCCCCCCCCCCCCGCCAGAGTGGGGGGGGGGGGGGCCCCCAAAAAAAAA
  AAAAAAAAAAAGGAGAGGGGGGCGCCGCCCCACACAAAAAAACAAAACACCCCCGCTGGG
  GGGCGGTCGTTGAGGGCGCCCCGCCNCNCTCCACACACAAACACACCCCCACCACCCCCC
  CCCCCAACCCCCGCGCCCGGGGGGGGCGGGGGGGGGGGGGGGGGCCCCCCGCCNAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAGTTTGTTTGTGGGGCTGGCGCGTCCCGCCCCCCCCAAC
  AACAACACAAAAAAAAAAAGGGAGGGGCGCGGGGGGGGCGGCGCGCGTTCTTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002997
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002997.4, NP_002988.3
• RefSeq Size: 3217 bp
• RefSeq ORF: 933 bp
• Locus ID: 6382
• UniProt ID: P18827
• Cytogenetics: 2p24.1
• Domains: Syndecan
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), ECM-receptor interaction
• Gene Summary: The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.