ST8SIA1 (NM_003034) Human Untagged Clone

CAT#: SC118261

ST8SIA1 (untagged)-Human ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST8SIA1
• Synonyms: GD3S; SIAT8; SIAT8-A; SIAT8A; ST8SiaI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003034, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCCCTGCGGGCGGGCCCGGCGACAAACGTCCAGAGGGGCCATGGCTGTACTGGCGTGGAAGTTCC
  CGCGGACCCGGCTGCCCATGGGAGCCAGTGCCCTCTGTGTCGTGGTCCTCTGTTGGCTCTACATCTTCCC
  CGTCTACCGGCTGCCCAACGAGAAAGAGATCGTGCAGGGGGTGCTGCAACAGGGCACGGCGTGGAGGAGG
  AACCAGACCGCGGCCAGAGCGTTCAGGAAACAAATGGAAGACTGCTGCGACCCTGCCCATCTCTTTGCTA
  TGACTAAAATGAATTCCCCTATGGGGAAGAGCATGTGGTATGACGGGGAGTTTTTATACTCATTCACCAT
  TGACAATTCAACTTACTCTCTCTTCCCACAGGCAACCCCATTCCAGCTGCCATTGAAGAAATGCGCGGTG
  GTGGGAAATGGTGGGATTCTGAAGAAGAGTGGCTGTGGCCGTCAAATAGATGAAGCAAATTTTGTCATGC
  GATGCAATCTCCCTCCTTTGTCAAGTGAATACACTAAGGATGTTGGATCCAAAAGTCAGTTAGTGACAGC
  TAATCCCAGCATAATTCGGCAAAGGTTTCAGAACCTTCTGTGGTCCAGAAAGACATTTGTGGACAACATG
  AAAATTTATAACCACAGTTACATCTACATGCCTGCCTTTTCTATGAAGACAGGAACAGAGCCATCTTTGA
  GGGTTTATTATACACTGTCAGATGTTGGTGCCAATCAAACAGTGCTGTTTGCCAACCCCAACTTTCTGCG
  TAGCATTGGAAAGTTCTGGAAAAGTAGAGGAATCCATGCCAAGCGCCTGTCCACAGGACTTTTTCTGGTG
  AGCGCAGCTCTGGGTCTCTGTGAAGAGGTGGCCATCTATGGCTTCTGGCCCTTCTCTGTGAATATGCATG
  AGCAGCCCATCAGCCACCACTACTATGACAACGTCTTACCCTTTTCTGGCTTCCATGCCATGCCCGAGGA
  ATTTCTCCAACTCTGGTATCTTCATAAAATCGGTGCACTGAGAATGCAGCTGGACCCATGTGAAGATACC
  TCACTCCAGCCCACTTCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003034 unedited
  TTAGGAGGGAGCCGAGCCTTCTCCCGGACCCTCGCCGAGGGCGACCGTGATGCTGCAGAA
  CCGGCGGGAGCGACTCGCCGCCGCCGCTCTCTGCGCATCTCTGAGACCCCAGCGCCCGCC
  TTCTGCAGGGGAAGCGACCATGGCCATAGATCGTGACTTCACCCCCAGCCACTTCCCCTA
  GAAAGAAATCCTTGGAAAAGTTGCATTTGAAAAAATCCTTGCGCTGACCTTTGGGGCCGA
  CGGGGCCGAAGAAGCGTGCGTGCGTTTGCAAGTAAGAGAACCAAAGGTGTGTGTGCATGG
  GGGGCTGGCGGTGGGGGACCCTCCGCTGCCACTTCGCCTAGCTTTGTGCTGAGGCCCCGG
  CCCCCGCCCCTGGGACGCCGGGGCTGCGATGAGCCCCTGCGGGCGGGCCCGGCGACAAAC
  GTCCAGAGGGGCCATGGCTGTACTGGCGTGGAAGTTCCCGCGGACCCGGCTGCCCATGGG
  AGCCAGTGCCCTCTGTGTCGTGGTCCTCTGTTGGCTCTACATCTTCCCCGTCTACCGGCT
  GCCCAACGAGAAAGAGATCGTGCAGGGGGGTGCTGCACAGGGGCACGGCGTGGAGAGGAA
  CCAGACCCGCGGCCAGAGCGTCAGGAAACAATGGAAGACTGCTGCGAACCTGCCCATCTC
  TTTGCTATGACTAANATGGAATTCCCTATGGGNAGAGCATGTGGTATGACGGGGAGTTTT
  ATACTCATTACCATTGACAATTAACTTACTCTCTCTTCCACAGGCNACCCCATTTCAGCT
  GCCATTGAAGAATGCGCCGTGGTGGGAAATAGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003034 unedited
  TTTTTTTTTTTTTTGAGACAGGGTCTTGCCCTGTCACCCAGGCTGGAGTGCAGTGGCACC
  ATCTTGGCTCACTGCAACCTCCACTTCCTGGGTTTAAGAGATTCTAGTCCAGATGATATT
  TTCAATGGAACTGTGATGTATGGGCTCTATGGAGCTCATTAATTACATTTTAACAATTAA
  AGTACTAATCTGAGTAACATTTTATTAACTTTTATATGCGTTTTTGTTAAGCCTTTTATA
  TTTTTAATGGTAGACTAGTAAAAATGTATTCTTTTTTAGGTAACTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003034
• Insert Size: 2290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003034.2, NP_003025.1
• RefSeq Size: 2117 bp
• RefSeq ORF: 1071 bp
• Locus ID: 6489
• UniProt ID: Q92185
• Cytogenetics: 12p12.1
• Domains: Glyco_transf_29
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• Gene Summary: Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. The encoded protein may be found in the Golgi apparatus and is a member of glycosyltransferase family 29. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.