Squalene Epoxidase (SQLE) (NM_003129) Human Untagged Clone

CAT#: SC118174

SQLE (untagged)-Human squalene epoxidase (SQLE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Squalene Epoxidase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118174 sequence for NM_003129 edited (data generated by NextGen Sequencing)
  ATGTGGACTTTTCTGGGCATTGCCACTTTCACCTATTTTTATAAGAAGTTCGGGGACTTC
  ATCACTTTGGCCAACAGGGAGGTCCTGTTGTGCGTGCTGGTGTTCCTCTCGCTGGGCCTG
  GTGCTCTCCTACCGCTGTCGCCACCGAAACGGGGGTCTCCTCGGGCGCCAGCAGAGCGGC
  TCCCAGTTCGCCCTCTTCTCGGATATTCTCTCAGGCCTGCCTTTCATTGGCTTCTTCTGG
  GCCAAATCCCCCCCTGAATCAGAAAATAAGGAGCAGCTCGAGGCCAGGAGGCGCAGAAAA
  GGAACCAATATTTCAGAAACAAGCTTAATAGGAACAGCTGCCTGTACATCAACATCTTCT
  CAGAATGACCCAGAAGTTATCATCGTGGGAGCTGGCGTGCTTGGCTCTGCTTTGGCAGCT
  GTGCTTTCCAGAGATGGAAGAAAGGTGACAGTCATTGAGAGAGACTTAAAAGAGCCTGAC
  AGAATAGTTGGAGAATTCCTGCAGCCGGGTGGTTATCATGTTCTCAAAGACCTTGGTCTT
  GGAGATACAGTGGAAGGTCTTGATGCCCAGGTTGTAAATGGTTACATGATTCATGATCAG
  GAAAGCAAATCAGAGGTTCAGATTCCTTACCCTCTGTCAGAAAACAATCAAGTGCAGAGT
  GGAAGAGCTTTCCATCACGGAAGATTCATCATGAGTCTCCGGAAAGCAGCTATGGCAGAG
  CCCAATGCAAAGTTTATTGAAGGTGTTGTGTTACAGTTATTAGAGGAAGATGATGTTGTG
  ATGGGAGTTCAGTACAAGGATAAAGAGACTGGAGATATCAAGGAACTCCATGCTCCACTG
  ACTGTTGTTGCAGATGGGCTTTTCTCCAAGTTCAGGAAAAGCCTGGTCTCCAATAAAGTT
  TCTGTATCATCTCATTTTGTTGGCTTTCTTATGAAGAATGCACCACAGTTTAAAGCAAAT
  CATGCTGAACTTATTTTAGCTAACCCGAGTCCAGTTCTCATCTACCAGATTTCATCCAGT
  GAAACTCGAGTACTTGTTGACATTAGAGGAGAAATGCCAAGGAATTTAAGAGAATACATG
  GTTGAAAAAATTTACCCACAAATACCTGATCACCTGAAAGAACCATTCTTAGAAGCCACT
  GACAATTCTCATCTGAGGTCCATGCCAGCAAGCTTCCTTCCTCCTTCATCAGTGAAGAAA
  CGAGGTGTTCTTCTTTTGGGAGACGCATNNNATATGAGGCATCCACTTACTGGTGGAGGA
  ATGACTGTTGCTTTTAAAGATATAAAACTATGGAGAAAACTGCTAAAGGGTATCCCTGAC
  CTTTATGATGATGCAGCTATTTTCGAGGCCAAAAAATCATTTTACTGGGCAAGAAAAACA
  TCTCATTCCTTTGTCGTGAATATCCTTGCTCAGGCTCTTTATGAATTATTTTCTGCCACA
  GATGATTCCCTGCATCAACTAAGAAAAGCCTGTTTTCTTTATTTCAAACTTGGTGGCGAA
  TGTGTTGCGGGTCCTGTTGGGCTGCTTTCTGTATTGTCTCCTAACCCTCTAGTTTTAATT
  GGACACTTCTTTGCTGTTGCAATCTATGCCGTGTATTTTTGCTTTAAGTCAGAACCTTGG
  ATTACAAAACCTCGAGCCCTTCTCAGTAGTGGTGCTGTATTGTACAAAGCGTGTTCTGTA
  ATATTTCCTCTAATTTACTCAGAAATGAAGTATATGGTTCATTAA
  
  Clone variation with respect to NM_003129.3
  1229 a=>n;1230 t=>n;1231 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003129 unedited
  CGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGAATATCTG
  GATTTCCTGGGCGAGGAGGAGCGAGTCTGCTCGGGAGCTGTTCCAGCAGGCGATTTTTAA
  ATACTGCTTTCTACGCCCTATACAACTTGGCTTCACATACTTTTACACTAACTTTATATG
  ATTTTTAAAAACTGGTCTGATCGGACTTCTCGTCCTGGGACACTGTTTACTGGAGTCTGG
  CCGGCTCTCCGTGCTCCTCTTGGTACCTCATTTTGGGGAGAACCTTAAACCCACTCGAGC
  AGATAATCTCCGCCTTGACCGGTGCCACCAAAGAAGCCTTGGAACCATGTGGACTTTTCT
  GGGCATTGCCACTTTCACCTATTTTTATAAGAAGTTCGGGGACTTCATCACTTTGGCCAA
  CAGGGAGGTCCTGTTGTGCGTGCTGGTGTTCCTCTCGCTGGGCCTGGTGCTCTCCTACCG
  CTGTCGCCACCGAAACGGGGGTCTCCTCGGGCGCCAGCAGAGCGGCTCCCAGTTCGCCCT
  CTTCTCGGATATTCTCTCAGGCCTGCCTTTCATTGGCTTCTTCTGGGCCAAATCCCCCCC
  TGAATCAGAAAATAAGGAGCAGCTCGAGGCCAGGAGGCGCAGAANAGGAACCAATATTTC
  AGAAACAAGCTTAATAGGAACAGCTGCCTGTACATCAACATCTTCTCAGAATGACCCAGA
  AGTTATCATCGTGGGAGCTGGCGTGCTTGGCTCTGCTTTGGCAGCTGTGCTTTCCAGAGA
  TGGAAGAAAGGTGACAGTCATTGAGAGAGACTTAAAGAGCCTGACAGAATANTNGNAGAA
  TTTCTGCAGCCGGGTGGTTATCATGGTCTCAAAGACCTTGGTCTTGNAGAACAGTGGAAG
  TCCTGATGCCCAGGTTGTAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003129 unedited
  AGAGTCGAGTTTTTTTTTTTTTTTTTTCATTCTTCATTTTATTTGCATGTCCCAACAACT
  CATCTCAAATACTAAATTCAAAAGAAAAACTGTAGTTCTCCTCAGCATTAGCACTAATTT
  ATGGTAACAATCATTTCTTTTAAATGTCTAACTTATTTAACCCCTTCATTTTAAATTGCA
  AATTAAAGCATGTATTTACATATTTATATACAAAAAACTTCAAAAACAAATTAATCCAAA
  TCTTGGTCCAAGAGTTTCCACTTTATAAGTGGTATGGTACTATGCTATATATATCCTCTT
  CCAAAAGTCTCTTAGGACTTGGTAAGTTCCAAATATTCATTCACAAATGGTTCCCCTTTA
  AGCTTAATGAACCATATACTTCATTTCTGAGTAAATTAGAGGAAATATTACAGAACACGC
  TTTGTACAATACAGCACCACTACTGAGAAGGGCTCGAGGTTTTGTAATCCAAGGTTCTGA
  CTTAAAGCANAAATACACGGCATAGATTGCAACAGCAAAGAAGTGTCCAATTAAAACTAG
  AGGGTTAGGAGACATTACAGAAAGCAGCCCACAGGACCCCGCACACATTCGCCACCAAGT
  TTGAATAAAGAAAACAGCTTTTCTAGTTGATGCAGGAATCATCTGTTGCAGAAATAATCA
  TAAAGAGCCTGACANGGATATCCCGACCAAGGATGAAATGNTTTTCTTGCCAGTAAANTG
  ATTTTTGGCCTCAAAAATGCCTGCTTATCATAAAGGTCAGGGATACCCTTTAACAGTTTT
  TCCCATAGTTTATTTCTTTAAAACACCAGCCATTCCCCACCAATAAGTGGATGCCTCAAT
  TTATTGCGTTCCCAAAAGAAGAACCCCTTGTTTCTTACTTGATAAGGAGAAAGAAACTTC
  TGGCATGGACCTCCAATGAAAAGCCAGGGCCTTTAAAAGGGCTTCTCAGGGACAAGATTT
  GGGGGAAATTTTTCAACCTGATTCTTTTATTCCTTGGCTTTCCCTTAAGCCACAATCTCC
  GTTACTGATAAATTCGGAAGAAACTGCCTGGTACTAAAAACCACCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003129
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003129.3, NP_003120.2
• RefSeq Size: 2989 bp
• RefSeq ORF: 1725 bp
• Locus ID: 6713
• UniProt ID: Q14534
• Cytogenetics: 8q24.13
• Domains: Monooxygenase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. [provided by RefSeq, Jul 2008]