Troponin C1 (TNNC1) (NM_003280) Human Untagged Clone

CAT#: SC118102

TNNC1 (untagged)-Human troponin C type 1 (slow) (TNNC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Troponin C1
• Synonyms: CMD1Z; CMH13; TN-C; TNC; TNNC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003280 edited
  ATGGATGACATCTACAAGGCTGCGGTAGAGCAGCTGACAGAAGAGCAGAAAAATGAGTTC
  AAGGCAGCCTTCGACATCTTCGTGCTGGGCGCTGAGGATGGCTGCATCAGCACCAAGGAG
  CTGGGCAAGGTGATGAGGATGCTGGGCCAGAACCCCACCCCTGAGGAGCTGCAGGAGATG
  ATCGATGAGGTGGACGAGGACGGCAGCGGCACGGTGGACTTTGATGAGTTCCTGGTCATG
  ATGGTTCGGTGCATGAAGGACGACAGCAAAGGGAAATCTGAGGAGGAGCTGTCTGACCTC
  TTCCGCATGTTTGACAAAAATGCTGATGGCTACATCGACCTGGATGAGCTGAAGATAATG
  CTGCAGGCTACAGGCGAGACCATCACGGAGGACGACATCGAGGAGCTCATGAAGGACGGA
  GACAAGAACAACGACGGCCGCATCGACTATGATGAGTTCCTGGAGTTCATGAAGGGTGTG
  GAGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003280 unedited
  ACGGTTCACTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGT
  GAGCCGCCAGCATGGATGACATCTACAAGGCTGCGGTAGAGCAGCTGACAGAAGAGCAGA
  AAAATGAGTTCAAGGCAGCCTTCGACATCTTCGTGCTGGGCGCTGAGGATGGCTGCATCA
  GCACCAAGGAGCTGGGCAAGGTGATGAGGATGCTGGGCCAGAACCCCACCCCTGAGGAGC
  TGCAGGAGATGATCGATGAGGTGGACGAGGACGGCAGCGGCACGGTGGACTTTGATGAGT
  TCCTGGTCATGATGGTTCGGTGCATGAAGGACGACAGCAAAGGGAAATCTGAGGAGGAGC
  TGTCTGACCTCTTCCGCATGTTTGACAAAAATGCTGATGGCTACATCGACCTGGATGAGC
  TGAAGATAATGCTGCAGGCTACAGGCGAGACCATCACGGAGGACGACATCGAGGAGCTCA
  TGAAGGACGGAGACAAGAACAACGACGGCCGCATCGACTATGATGAGTTCCTGGAGTTCA
  TGAAGGGTGTGGAGTAGATGCTGACCTTCACCCAGAGCTGCCTATGCCCAGCCTCCAACT
  CCAGCTGAGTCCTGGGTGGGGGGAGGGNNNNNNNNCNCNNTCCTCCCCACCTCCTCTCCC
  TATCNCCTCCCCTACTTCCCCTCCTTTCCTCCTCCTCTCCCCCCTATTTTATTTATACAA
  TAAAAAACAAAAACCTAATCCCACCCTTCCTACACCTAAATTATTTCAAAACAACATCAT
  TAACTCACTTTCTATCCACACTACTCTCCTATCCTCTCTTACCCCTACTCCCTATCTCCC
  TTTCAATCCCCACCTTATTATTTCCTTATTTCTCCTCCTAATCACATTACAATAATCTCT
  CTACTTATTCCACTAACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003280 unedited
  CCGTTTGCGGGCTTTTTTGCATCTCCCGGACGCATCGGGGCGGGAGTCGGGGGATTTGGG
  GTTGAGGACATGGCCAGGCTCAGGTCCTGGGACCCCGACCCCCTCCCCAACCCCAGGACT
  CAGCTGGAGTTGGAGGCTGGGCATAGGCAGCTCTGGGTGAAGGTCAGCATCTACTCCACA
  CCCTTCATGAACTCCAGGAACTCATCATAGTCGATGCGGCCGTCGTTGTTCTTGTCTCCG
  TCCTTCATGAGCTCCTCGATGTCGTCCTCCGTGATGGTCTCGCCTGTAGCCTGCAGCATT
  ATCTTCAGCTCATCCAGGTCGATGTAGCCATCAGCATTTTTGTCAAACATGCGGAAGAGG
  TCACACAGCTCCTCCTCAGATTTCCCTTTGCTGTCGTCCTTCATGCACCGAACCATCATG
  ACCAGGAACTCATCAAAGTCCACCGTGCCGCTGCCGTCCTCGTCCACCTCATCGATCATC
  TCCTGCAGCTCCTCAGGGGTGGGGCTCTGCCCCACCATCCTCATCACCTTGCCCAGCTCC
  TTGGTGCTGATGCACCCATCCTCACCGCCCAGCACCAACATGCCCAACGCTGCCTTGAAC
  TCATTGTCCTGCCGTTGTGCCAGCTGCTCTACCGCAACCTTGGACATGCCATGGATGCTC
  GCTGGGTCACCCTGGTGCCCCCATCCGCGGCCGCCCTATAGTGCAGCGCCCTCACAAAAT
  TGCCGCCGCCCCACTAAACCGGCCCCGGGTTATCTAGACCTCCCCCCGGGCCCAGCTACC
  GCCCATTCGCGCGAACGGGCCGGCGGTCTTACCCCCTTGCGAACGTCGCGCTGCTACTCC
  CCCCACACAGACCGCGTTGGCCGCGCTGCCGCCCACCTTCCCAACCGCGTGGGCATACCG
  TCTCTCCCCCATGATCCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003280
• Insert Size: 820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003280.1, NP_003271.1
• RefSeq Size: 686 bp
• RefSeq ORF: 486 bp
• Locus ID: 7134
• UniProt ID: P63316
• Cytogenetics: 3p21.1
• Domains: EFh
• Protein Pathways: Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
• Gene Summary: Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]