Trefoil Factor 3 (TFF3) (NM_003226) Human Untagged Clone

CAT#: SC118100

TFF3 (untagged)-Human trefoil factor 3 (intestinal) (TFF3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Trefoil Factor 3
• Synonyms: ITF; P1B; TFI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003226, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGCGAGTCCTGAGCTGCGTCCCGGAGCCCACGGTGGTCATGGCTGCCAGAGCGCTCTGCATGCTGG
  GGCTGGTCCTGGCCTTGCTGTCCTCCAGCTCTGCTGAGGAGTACGTGGGCCTGTCTGCAAACCAGTGTGC
  CGTGCCAGCCAAGGACAGGGTGGACTGCGGCTACCCCCATGTCACCCCCAAGGAGTGCAACAACCGGGGC
  TGCTGCTTTGACTCCAGGATCCCTGGAGTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCT
  TCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003226 unedited
  NGTTCACATTAGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGCGGTGGTCAT
  GGCTGCCAGAGCGTTCTGCATGCTGGGGCTGGTCCTGGCCTTGCTGTCCTCCAGCTCTGC
  TGAGGAGTATGTGGGCCTGTCTGCAAACCAGTGTGCCGTGCCAGCCAAGGACAGGGTGGA
  CTGCGGCTACCCCCATGTCACCCCCAAGGAGTGCAACAACCGGGGCTGCTGCTTTGACTC
  CAGGATCCCTGGAGTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTG
  AGGCACCTCCAGCTGCCCCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTG
  TGATTGCTGCCAGGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCT
  TCTGCTGAAAGTTCATATCTGGAGCCTGATGTCTTAACGAATAAAGGTCCCATGCTCCAC
  CCGAGGACAGTTCTTCGTGCCTGAGAAAAANAAAACAAAAAACAAAAAAAAAAAAAAACC
  TCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCC
  CTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAG
  CCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA
  TTATGGGGTGGAGCGGGCGGGGTATGGAACCCAAGGGCTCGTTCGCCAAATAACATCTTT
  ATGGTCCTGGAGAACACTTTGGGTTACTATCTCGTTCCGTACCCGACCACTTCCCTCCAT
  CACTCGCCTATCCCCCCTCCCTGCAACAACGAAATTTATTTGCACAGTCCCTCACATACT
  TTTGGTACCTCTCCTCTCACGCCCACCTTCCATCTACTCCTTCTCACTCCGCCCCACCCC
  CG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003226 unedited
  TTTTATGGAACGAGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTCTCAGGCACAAAAAACTGTCCTCGGGTGGAGCATGGGACCTTTATTCGTTAAA
  ACATCAGGCTCCAAATATGAACTTTCAGCAAAAGCGCTTGCCGGGAGCAAAGGGACAAAA
  AAGCTGAAATGAACAGTGCCTGGCAGCAATCACAGCCGGGCAAGGGTGCTCCGAGCCTCG
  CATCCCCCGGCCGGGGGCAGCTGGAGGGGCCTCAAAAGGGGCATTCTGCTTCCTGCAGGG
  GCTTGAAACACCAAGGCACTCCAGGGATCCTGGAGTCAAAGCAGCAGCCCCGGTTGTTGC
  ACTCCTTGGGGGTGACATGGGGGTAGCCGCAGTCCACCCTGTCCTTGGCTGGCACGGCAC
  ACTGGTTTGCAAACAGGCCCACGTACTCCTCAACAAAGCTGGAGGACAGCAAGGCCAGGA
  CCAGCCCCAGCATGCAAAGCGCTCTGGCAGCCATGACCACCGCTCGTGCCGAATTCGCGG
  CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT
  ATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACG
  ACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG
  GTGGAGACTTGGAAATCCCCGTGAGTTAAACCGCTATCCACGCCCATTGGTGTACTGCCA
  AACCGCATACCATGGGTATAGCGATGACTAATACGTAAATGTACTGCCAAGTAGGAAAGT
  CCCGTAAGGTCATGGTCTGGGCATATGGCCGGCCGGCCCTTTACCGNATTGACGTCATTA
  GGGGGCGGCCTGGCATATAATCACTTGATGTCTGGCAAGGGGCAGTTTACGGAAATACTC
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003226
• Insert Size: 480 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003226.1, NP_003217.1
• RefSeq Size: 1054 bp
• RefSeq ORF: 225 bp
• Locus ID: 7033
• UniProt ID: Q07654
• Cytogenetics: 21q22.3
• Domains: PD
• Protein Families: Secreted Protein
• Gene Summary: Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]