DEGS1 (NM_003676) Human Untagged Clone

CAT#: SC117856

DEGS1 (untagged)-Human degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) (DEGS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DEGS1
• Synonyms: DEGS; DEGS-1; Des-1; DES1; FADS7; HLD18; MIG15; MLD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117856 sequence for NM_003676 edited (data generated by NextGen Sequencing)
  ATGGGGAGCCGCGTCTCGCGGGAAGACTTCGAGTGGGTCTACACCGACCAGCCGCACGCC
  GACCGGCGCCGGGAGATCCTGGCAAAGTATCCAGAGATAAAGTCCTTGATGAAACCTGAT
  CCCAATTTGATATGGATTATAATTATGATGGTTCTCACCCAGTTGGGTGCATTTTACATA
  GTAAAAGACTTGGACTGGAAATGGGTCATATTTGGGGCCTATGCGTTTGGCAGTTGCATT
  AACCACTCAATGACTCTGGCTATTCATGAGATTGCCCACAATGCTGCCTTTGGCAACTGC
  AAAGCAATGTGGAATCGCTGGTTTGGAATGTTTGCTAATCTTCCTATTGGGATTCCATAT
  TCAATTTCCTTTAAGAGGTATCACATGGATCATCATCGGTACCTTGGAGCTGATGGCGTC
  GATGTAGATATTCCTACCGATTTTGAGGGCTGGTTCTTCTGTACCGCTTTCAGAAAGTTT
  ATATGGGTTATTCTTCAGCCTCTCTTTTATGCCTTTCGACCTCTGTTCATCAACCCCAAA
  CCAATTACGTATCTGGAAGTTATCAATACCGTGGCACAGGTCACTTTTGACATTTTAATT
  TATTACTTTTTGGGAATTAAATCCTTAGTCTACATGTTGGCAGCATCTTTACTTGGCCTG
  GGTTTGCACCCAATTTCTGGACATTTTATAGCTGAGCATTACATGTTCTTAAAGGGTCAT
  GAAACTTACTCATATTATGGGCCTCTGAATTTACTTACCTTCAATGTGGGTTATCATAAT
  GAACATCATGATTTCCCCAACATTCCTGGAAAAAGTCTTCCACTGGTGAGGAAAATAGCA
  GCTGAATACTATGACAACCTCCCTCACTACAATTCCTGGATAAAAGTACTGTATGATTTT
  GTGATGGATGATACAATAAGTCCCTACTCAAGAATGAAGAGGCACCAAAAAGGAGAGATG
  GTGCTGGAGTAA
  
  Clone variation with respect to NM_003676.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003676 unedited
  NGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAGCCGC
  CGCCGCCGCCGCCACCTCTGAGCAGCCGGCTGGGAGCGAGAGCCGACAGCTAGTCTGCAA
  GCCACCGCTGTCGCCATGGGGAGCCGCGTCTCGCGGGAAGACTTCGAGTGGGTCTACACC
  GACCAGCCGCACGCCGACCGGCGCCGGGAGATCCTGGCAAAGTATCCAGAGATAAAGTCC
  TTGATGAAACCTGATCCCAATTTGATATGGATTATAATTATGATGGTTCTCACCCAGTTG
  GGTGCATTTTACATAGTAAAAGACTTGGACTGGAAATGGGTCATATTTGGGGCCTATGCG
  TTTGGCAGTTGCATTAACCACTCAATGACTCTGGCTATTCATGAGATTGCCCACAATGCT
  GCCTTTGGCAACTGCAAAGCAATGTGGAATCGCTGGTTTGGAATGTTTGCTAATCTTCCT
  ATTGGGATTCCATATTCAATTTCCTTTAAGAGGTATCACATGGATCATCATCGGTACCTT
  GGAGCTGATGGCGTCGATGTAGATATTCCTACCGATTTTGAGGGCTGGTTCTTCTGTACC
  GCTTTCAGAAAGTTTATATGGGTTATTCTTCAGCCTCTCTTTTATGCCTTTCGACCTCTG
  TTCATCAACCCCAAACCAATTACGTATCTGGAAGTTATCAATACCGTGGCACAGGTCACT
  TTTGACATTNTAATTTATTACTTTNTGGGAATTAAATCCTTAGTCTACATGNTGGCAGCA
  TCTTTACTTGGCCTGGGTTTGCACCCCATTTCTGGACATTNTATAGCTGAGCATTTACAT
  GTCTTAAAGGGTCATGAAACTTACTCATATTATGGGCCTCTGAATTTACTTACCTTCATG
  TGGGTTATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003676 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAATTTGAATGGTGGAAATTCA
  GTAAGTACTTTATTTATAAGTAAGGCTGTACAGGATTCAGCAGTGTCTAATTCGGGTCCC
  ATATAGAAGTTAAAATTTTTAAATGAGTAATTCAGCTTACAGGGAAATATATTTTCATCT
  CAATCTTTTATGCTATATCATACCTCAGGCTTTTTAAAAATTATTTTTGAGATGAAATTT
  TAAAGTTCCCCCGGTACCCAAGTTACACTATTATATAGTATAAAGATAGTGAAATACAAA
  AATGTAACAAATACTAATACACTGTGATTAATAGCTGTTTATTAGCTTTTTAATAAAGCA
  AAACTTTATAGTGAAATAAACTAGAAACAGCTTTTTATTTTTTATATATATTTATTTATT
  TTCGAGATGGGGTCTTGCTCTGTCGCCCAGGGTGGAGTGCAGCGGCACAATTCTTGCGTC
  ACTGCAGCCTCTGCCTNCGCCTCCTGGGTTCAAGCAATTCTTCCCCCTTAGCCCTCCCAT
  GTAGCCTGGGATTACAGGGCATGTGCCCGCCACGTCCCGGCTAATTCTTGCATCCCTAGT
  ACAGCATGGGGCTTCACCCGTGTTGGCCCAGGCTGGCCTTCGACCCCTTGACCTTAGGTG
  ATCCACCCCACCTGGCCCTCCCAAGGGCTGGGACTATAGGCATGACCCCCCGTCCCCGGC
  GACAAAACTACTCAAAGCCTCTTCAAGGACCGGCTATAAAATGCCGACCTGACCGAAACC
  TTCCCATGGACCACCCCCCCCAACACAAGTTTCTTGCGAGGAAACCGGGCCACACGAACC
  CGGGTGACTGTTTAAAGCCACATTCACTCCTTGATCAACCGGACCTCTACCTGGAAATGG
  GCCCGGGGAGCTTTGGCCTCCCTGGTTCAAGTTAAAATGCAAACCCCCTTTACCCACTAA
  TCCGGGAAACACCCCTGGGCCCATGTATTTACTCCACCCCTCTTCTTTTGGGCCCTTCTT
  CTCGAGGGGCCTCTGGTTACCCTACAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003676
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003676.2, NP_003667.1
• RefSeq Size: 2058 bp
• RefSeq ORF: 972 bp
• Locus ID: 8560
• UniProt ID: O15121
• Cytogenetics: 1q42.11
• Domains: FA_desaturase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: This gene encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. [provided by RefSeq, Mar 2010]