LGR5 (NM_003667) Human Untagged Clone

CAT#: SC117852

LGR5 (untagged)-Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LGR5
• Synonyms: FEX; GPR49; GPR67; GRP49; HG38
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117852 sequence for NM_003667 edited (data generated by NextGen Sequencing)
  ATGGACACCTCCCGGCTCGGTGTGCTCCTGTCCTTGCCTGTGCTGCTGCAGCTGGCGACC
  GGGGGCAGCTCTCCCAGGTCTGGTGTGTTGCTGAGGGGCTGCCCCACACACTGTCATTGC
  GAGCCCGACGGCAGGATGTTGCTCAGGGTGGACTGCTCCGACCTGGGGCTCTCGGAGCTG
  CCTTCCAACCTCAGCGTCTTCACCTCCTACCTAGACCTCAGTATGAACAACATCAGTCAG
  CTGCTCCCGAATCCCCTGCCCAGTCTCCGCTTCCTGGAGGAGTTACGTCTTGCGGGAAAC
  GCTCTGACATACATTCCCAAGGGAGCATTCACTGGCCTTTACAGTCTTAAAGTTCTTATG
  CTGCAGAATAATCAGCTAAGACACGTACCCACAGAAGCTCTGCAGAATTTGCGAAGCCTT
  CAATCCCTGCGTCTGGATGCTAACCACATCAGCTATGTGCCCCCAAGCTGTTTCAGTGGC
  CTGCATTCCCTGAGGCACCTGTGGCTGGATGACAATGCGTTAACAGAAATCCCCGTCCAG
  GCTTTTAGAAGTTTATCGGCATTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCAC
  ATACCAGACTATGCCTTTGGAAACCTCTCCAGCTTGGTAGTTCTACATCTCCATAACAAT
  AGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGGCTCCACAGCCTAGAGACTTTAGAT
  TTAAATTACAATAACCTTGATGAATTCCCCACTGCAATTAGGACACTCTCCAACCTTAAA
  GAACTAGGATTTCATAGCAACAATATCAGGTCGATACCTGAGAAAGCATTTGTAGGCAAC
  CCTTCTCTTATTACAATACATTTCTATGACAATCCCATCCAATTTGTTGGGAGATCTGCT
  TTTCAACATTTACCTGAACTAAGAACACTGACTCTGAATGGTGCCTCACAAATAACTGAA
  TTTCCTGATTTAACTGGAACTGCAAACCTGGAGAGTCTGACTTTAACTGGAGCACAGATC
  TCATCTCTTCCTCAAACCGTCTGCAATCAGTTACCTAATCTCCAAGTGCTAGATCTGTCT
  TACAACCTATTAGAAGATTTACCCAGTTTTTCAGTCTGCCAAAAGCTTCAGAAAATTGAC
  CTAAGACATAATGAAATCTACGAAATTAAAGTTGACACTTTCCAGCAGTTGCTTAGCCTC
  CGATCGCTGAATTTGGCTTGGAACAAAATTGCTATTATTCACCCCAATGCATTTTCCACT
  TTGCCATCCCTAATAAAGCTGGACCTATCGTCCAACCTCCTGTCGTCTTTTCCTATAACT
  GGGTTACATGGTTTAACTCACTTAAAATTAACAGGAAATCATGCCTTACAGAGCTTGATA
  TCATCTGAAAACTTTCCAGAACTCAAGGTTATAGAAATGCCTTATGCTTACCAGTGCTGT
  GCATTTGGAGTGTGTGAGAATGCCTATAAGATTTCTAATCAATGGAATAAAGGTGACAAC
  AGCAGTATGGACGACCTTCATAAGAAAGATGCTGGAATGTTTCAGGCTCAAGATGAACGT
  GACCTTGAAGATTTCCTGCTTGACTTTGAGGAAGACCTGAAAGCCCTTCATTCAGTGCAG
  TGTTCACCTTCCCCAGGCCCCTTCAAACCCTGTGAACACCTGCTTGATGGCTGGCTGATC
  AGAATTGGAGTGTGGACCATAGCAGTTCTGGCACTTACTTGTAATGCTTTGGTGACTTCA
  ACAGTTTTCAGATCCCCTCTGTACATTTCCCCCATTAAACTGTTAATTGGGGTCATCGCA
  GCAGTGAACATGCTCACGGGAGTCTCCAGTGCCGTGCTGGCTGGTGTGGATGCGTTCACT
  TTTGGCAGCTTTGCACGACATGGTGCCTGGTGGGAGAATGGGGTTGGTTGCCATGTCATT
  GGTTTTTTGTCCATTTTTGCTTCAGAATCATCTGTTTTCCTGCTTACTCTGGCAGCCCTG
  GAGCGTGGGTTCTCTGTGAAATATTCTGCAAAATTTGAAACGAAAGCTCCATTTTCTAGC
  CTGAAAGTAATCATTTTGCTCTGTGCCCTGCTGGCCTTGACCATGGCCGCAGTTCCCCTG
  CTGGGTGGCAGCAAGTATGGCGCCTCCCCTCTCTGCCTGCCTTTGCCTTTTGGGGAGCCC
  AGCACCATGGGCTACATGGTCGCTCTCATCTTGCTCAATTCCCTTTGCTTCCTCATGATG
  ACCATTGCCTACACCAAGCTCTACTGCAATTTGGACAAGGGAGACCTGGAGAATATTTGG
  GACTGCTCTATGGTAAAACACATTGCCCTGTTGCTCTTCACCAACTGCATCCTAAACTGC
  CCTGTGGCTTTCTTGTCCTTCTCCTCTTTAATAAACCTTACATTTATCAGTCCTGAAGTA
  ATTAAGTTTATCCTTCTGGTGGTAGTCCCACTTCCTGCATGTCTCAATCCCCTTCTCTAC
  ATCTTGTTCAATCCTCACTTTAAGGAGGATCTGGTGAGCCTGAGAAAGCAAACCTACGTC
  TGGACAAGATCAAAACACCCAAGCTTGATGTCAATTAACTCTGATGATGTCGAAAAACAG
  TCCTGTGACTCAACTCAAGCCTTGGTAACCTTTACCAGCTCCAGCATCACTTATGACCTG
  CCTCCCAGTTCCGTGCCATCACCAGCTTATCCAGTGACTGAGAGCTGCCATCTTTCCTCT
  GTGGCATTTGTCCCATGTCTCTAA
  
  Clone variation with respect to NM_003667.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003667 unedited
  NATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGATGCTGCTCCACACC
  GCTCAGGCCGCGAGCAGCAGCAAGGCGCACCGCCACTGTCGCCGCTGCAGCCAGGGCTGC
  TCCGAAGGCCGGCGTGGCGGCAACCGGCACCTCTGTCCCCGCCGCGCTTCTCCTCGCCGC
  CCACGCCGTGGGGTCAGGAACGCGGCGTCTGGCGCTGCAGACGCCCGCTGAGTTGCAGAA
  GCCCACGGAGCGGCGCCCGGCGCGCCACGGCCCGTAGCAGTCCGGTGCTGCTCTCCGCCC
  GCGTCCGGCTCGTGGCCCCCTACTTCGGGCACCATGGACACCTCCCGGCTCGGTGTGCTC
  CTGTCCTTGCCTGTGCTGCTGCAGCTGGCGACCGGGGGCAGCTCTCCCAGGTCTGGTGTG
  TTGCTGAGGGGCTGCCCCACACACTGTCATTGCGAGCCCGACGGCAGGATGTTGCTCAGG
  GTGGACTGCTCCGACCTGGGGCTCTCGGAGCTGCCTTCCAACCTCAGCGTCTTCACCTCC
  TACCTAGACCTCAGTATGAACAACATCAGTCAGCTGCTCCCGAATCCCCTGCCCAGTCTC
  CGCTTCCTGGAGGAGTTACGTCTTGCGGGAAACGCTCTGACATACATTCCCAAGGGAGCA
  TTCACTGGCCTTTACAGTCTTAAAGTTCTTATGCTGCAGAATAATCAGCTAAGACACGTA
  CCCACAGAAGCTCTGCAGAATTTGCGAAGCCTTCAATCCTGCGTCTGGATGCTAACCCAT
  CAGCTATGTGCCCCAAGCTGGTTTCAGTGGCCTGCATTCCTGAGGCACCTGTGCCTGGAT
  GACCATGCGTTACAGAAATCCCCGCCAGCTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003667 unedited
  ATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAATAGTCACAC
  ATTTTATTACAAATTTATTTTTTACCCAATAGAAAAGCAAATTTGGAATCTATTTACAAG
  TACTATATATTTACATATATACAGTTAATGAATTTAAAGAAAATGGGCAGAGAAACACAT
  TATAAATCAAAGAATATGCCACTGTACAAGGTTTATTATCATTATCATGGTCCTTAATGT
  TACTGAACCTTTACTATAGTAATAAATACAGTTCTATATTTACACATCTTATAAAACATC
  TCATAAATGTATTTTTTCAAATCCAAGTTAAAACATCTGATCAAAATAAACATGCTTATA
  TAAAAATAAATCTACCTAACAGCCATTTGGTTTGGATGTATTGAAGCTAATATAGGATAA
  TAGAAGGTAAGAATTAATACTTTGACTTTTCTTATTTAATAACTTGCTTCTTAAAATACC
  TAACACAGTATTAATATGGAATAAGCAGAGAAGTAATGTTCCTAACATCAAGTGGGTTAT
  CCAGAGAGAACACAGCTAAAACCAAGCTAAATAAACAGGATAATACGTTACTGAGTCTCT
  TGAGTCCAAAGTGGTGTCAGATATTGGGTTTGCCAGAGCTACTAGAGATACATGTGTAAG
  AAGTTGTATCAGTGGACTTAATTTATGTAATGTGCACATTTGATCATTAAGATGCACATC
  AGTTTGAATCAACTGATAAAACTTATTGCAAAAATTCTTTACTAACCCAGAAAAAAATCC
  CAGATTGCTTACTTTNNCTTTTCAGGTATGTCATTGCTGGCAGTAGGAATTCCCTTCTGA
  GCTTTGCNGCACCAGGAGTTAAAANNNCAATCAGATAGACATACGTCACCTGTGCATGAA
  TGCCTTANNNTACATTAAGAATTTTGGCCAGTTTTTCTCCAAATACTTGNAGCAGTTTTA
  CCCATGAGGGTGGAACCATCTAGGGCTGAACATAAACCTGGTATCCCTAAAATGACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003667
• Insert Size: 2724 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003667.2, NP_003658.1
• RefSeq Size: 2880 bp
• RefSeq ORF: 2724 bp
• Locus ID: 8549
• UniProt ID: O75473
• Cytogenetics: 12q21.1
• Domains: 7tm_1, LRRNT, LRR, LRR_TYP, LRR_PS
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.