Carbohydrate sulfotransferase 1 (CHST1) (NM_003654) Human Untagged Clone

CAT#: SC117840

CHST1 (untagged)-Human carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Carbohydrate sulfotransferase 1
• Synonyms: C6ST; GST-1; KS6ST; KSGal6ST; KSST
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003654, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAATGTTCCTGGAAGGCCGTCCTCCTCCTTGCCCTGGCCTCCATTGCCATCCAGTACACGGCCATCC
  GCACCTTCACCGCCAAGTCCTTTCACACCTGCCCCGGGCTGGCAGAGGCCGGGCTGGCCGAGCGACTGTG
  CGAGGAGAGCCCCACCTTCGCCTACAACCTCTCCCGCAAGACCCACATCCTCATCCTGGCCACCACGCGC
  AGTGGCTCCTCCTTCGTGGGCCAGCTCTTCAACCAGCACCTGGACGTCTTCTACCTGTTTGAGCCCCTCT
  ACCACGTCCAGAACACGCTCATCCCCCGCTTCACCCAGGGCAAGAGCCCGGCCGACCGGCGGGTCATGCT
  AGGCGCCAGCCGCGACCTCCTGCGGAGCCTCTACGACTGCGACCTCTACTTCCTGGAGAACTACATCAAG
  CCGCCGCCGGTCAACCACACCACCGACAGGATCTTCCGCCGCGGGGCCAGCCGGGTCCTCTGCTCCCGGC
  CTGTGTGCGACCCTCCGGGGCCAGCCGACCTGGTCCTGGAGGAGGGGGACTGTGTGCGCAAGTGCGGGCT
  ACTCAACCTGACCGTGGCGGCCGAGGCGTGCCGCGAGCGCAGCCACGTGGCCATCAAGACGGTGCGCGTG
  CCCGAGGTGAACGACCTGCGCGCCCTGGTGGAAGACCCGCGATTAAACCTCAAGGTCATCCAGCTGGTCC
  GAGACCCCCGCGGCATTCTGGCTTCGCGCAGCGAGACCTTCCGCGACACGTACCGGCTCTGGCGGCTCTG
  GTACGGCACCGGGAGGAAACCCTACAACCTGGACGTGACGCAGCTGACCACGGTGTGCGAGGACTTCTCC
  AACTCCGTGTCCACCGGCCTCATGCGGCCCCCGTGGCTCAAGGGCAAGTACATGTTGGTGCGCTACGAGG
  ACCTGGCTCGGAACCCTATGAAGAAGACCGAGGAGATCTACGGGTTCCTGGGCATCCCGCTGGACAGCCA
  CGTGGCCCGCTGGATCCAGAACAACACGCGGGGCGACCCCACCCTGGGCAAGCACAAATACGGCACCGTG
  CGAAACTCGGCGGCCACGGCCGAGAAGTGGCGCTTCCGCCTCTCCTACGACATCGTGGCCTTTGCCCAGA
  ACGCCTGCCAGCAGGTGCTGGCCCAGCTGGGCTACAAGATCGCCGCCTCGGAGGAGGAGCTGAAGAACCC
  CTCGGTCAGCCTGGTGGAGGAGCGGGACTTCCGCCCCTTCTCGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003654 unedited
  CAGAATTTGTATACGACTTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGCGCGC
  GGAGTCGGAGGAAGAGGAGGGAGGAGGGAGGCCGAGGAGGAGGGCGCGGGAGGCGGAGGA
  TGCCGCCGCGGCTGCTGCCGCCGCCGCCACCCGCGGGTCCCCGGCGACCCTGACTCCAGA
  CCCGAGGATGGAGCCGGCGCTGGGCGCTGCAGCTGCTCCCGGCGCGTCCCCGACCAGGTA
  GCTGGTGTCACTTCGGTGTGGTTGGAAGAAGACTTTCTCCCCAGCTGCATTCCCGGAGGC
  GCCCTTTCGACCTGGAGGCCGGGTCTGCTGGCCACAGGGCTGCCGCACTGGCTGGGACTG
  CCAGCTGGGCCTGGAGACGCTGGTGGCTGTGGACTCCCCAGCTTGGAGCAGTCCCTCTTT
  GACCTCACCCCTTGGAGAAGCAGCCCCATGAAGGTGCCCAGCCATGCAATGTTCCTGGAA
  GGCCGTCCTCCTCCTTGCCCTGGCCTCCATTGCCATCCAGTACACGGCCATCCGCACCTT
  CACCGCCAAGTCCTTTCACACCTGCCCCGGGCTGGCAGAGGCCGGGCTGGCCGAGCGACT
  GTGCGAGGAGAGCCCCACCTTCGCCTACAACCTCTCCCGCAAGACCCACATCCTCATCCT
  GGCCACCACGCGCAGTGGCTCCTCCTTCGTGGGCCAGCTCTTCAACCAGCACCTGGACGT
  CTTCTACCTGTTTGAGCCCCTCTACCACGTNCAGAACACGCTCATCCCCCGCTTCACCCA
  GGGCAAGAGCCCGGCCGACCGGCGGGTCATGCTANGCGCCAGCCGCGACCTCCTGCGGAG
  CCTCTACGACTGCGACCTCTACTTCCTGGAGAACTACATCAAGCCGNCGNCGGGTCACCA
  CACCACCGACAGGATCTTNCGNCGCGGGGCCAGCCGGGTCTCTGCTCCGGCCTGGTGCGA
  CCCTCGGGCACAGCTGTNNNTTTNGTGGAGGAGGGGACTGTGGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003654 unedited
  TGCTTTTTATTACAGNAAACACAGNTAAAGCATTTCAATTTCAATGTTCATTTAGCAAAC
  TGATCCACTCTTTTTTTTTCTTTTTGGCACAAAGAAATATCACAGATGGACCCCGAGATC
  AATCAGAAGCTCATAAGATTCATCAGTCATCACTGGTCCAGGGGCGGCCACGAGACTCAG
  ACAGACAGACAGCATCACCACAGACTGGAAACAGAAACAGGTCCACGTTCACCTCACAGT
  AAAAACCTGCCTCACCGACAGGCAAGGGCGGGCAGGAGGGGGCAGTTTCGCTGCTCTAAG
  GGGGGAAATGGGCGTCAGGGGCAGGAGGCAGGGATGGGGAAAGGATGGACACCATTCATA
  AATTAGAGACAAGTGGTCCTCTTGTTTGTATCCTCTTACCTTGAATAGTAAGACAGTGCA
  AAAACTAGATACCCGACCACTCACCCATTCAAAAGCTTGAGAAGTCGCCTCGCGAGTGCC
  CGTGTGCGTGTGTGGATGTGTAAATGTGGTGCTTGTAAACATTGTCACCGTCCGCACAGA
  GACCCAACATCCATGTGTCTGAATGGGGTGGGGGGGGGCGGGACCCTACTTCAGGCGCCC
  TCTGCCCCAGTGATTCCCGTCCAAGACGTAGTGCAAATTTCAGAGACAAAAAAGGGGCAN
  AAGGGAGTGGGGTGAGCTGGGGGCAGGAAGGACACGAAGATGATGTGGGAGAGGGAGGGG
  TTAATAAGGCAACAGTTAAAAACGGTCCATTTTATCAAAACCCGACACTTGCGCCNTCCG
  NCCNCACCCGNACCGNCCGGGTCACGAGAAGGGGCGGNAGTNCCGCTCCTCCACCAGCTG
  ACCGAGGGGTTCTTCAGCTCCTCCTCCGAAGCGGCGATCTTGTAGCCCANTTGGCCCGCA
  CCTGCTGGCAGGCGTCTTGGCAAAGCCACATGTCGAGGAGAGGCGGAGCGCCATTTTCGG
  CGTGGCCGCGAGTTTCACGGGCCGCATTGGCTGCCCAGGTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003654
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003654.2, NP_003645.1
• RefSeq Size: 2734 bp
• RefSeq ORF: 1236 bp
• Locus ID: 8534
• UniProt ID: O43916
• Cytogenetics: 11p11.2
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Keratan sulfate biosynthesis, Metabolic pathways
• Gene Summary: This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]