HYAL2 (NM_003773) Human Untagged Clone

CAT#: SC117754

HYAL2 (untagged)-Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HYAL2
• Synonyms: LUCA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117754 sequence for NM_003773 edited (data generated by NextGen Sequencing)
  ATGCGGGCAGGCCCAGGCCCCACCGTTACATTGGCCCTGGTGCTGGCGGTGGCATGGGCC
  ATGGAGCTCAAGCCCACAGCACCACCCATCTTCACTGGCCGGCCCTTTGTGGTAGCGTGG
  GACGTGCCCACACAGGACTGTGGCCCACGCCTCAAGGTGCCACTGGACCTGAATGCCTTT
  GATGTGCAGGCCTCACCTAATGAGGGTTTTGTGAACCAGAATATTACCATCTTCTACCGC
  GACCGTCTAGGCCTGTATCCACGCTTCGATTCTGCCGGAAGGTCTGTGCATGGTGGTGTG
  CCACAGAATGTCAGCCTTTGGGCACACCGGAAGATGCTGCAGAAACGTGTGGAGCACTAC
  ATTCGGACACAGGAGTCTGCGGGGCTGGCGGTCATCGACTGGGAGGACTGGCGACCTGTG
  TGGGTGCGCAACTGGCAGGACAAAGATGTGTATCGCCGGTTATCACGCCAGCTAGTGGCC
  AGTCGTCACCCTGACTGGCCTCCAGACCGCATAGTCAAACAGGCACAATATGAGTTTGAG
  TTCGCAGCACAGCAGTTCATGCTGGAGACACTGCGTTATGTCAAGGCAGTGCGGCCCCGG
  CACCTCTGGGGCTTCTACCTCTTTCCTGACTGCTACAATCATGATTATGTGCAGAACTGG
  GAGAGCTACACAGGCCGCTGCCCTGATGTTGAGGTGGCCCGCAATGACCAGCTGGCCTGG
  CTGTGGGCTGAGAGCACGGCCCTCTTCCCGTCTGTCTACCTGGACGAGACACTTGCTTCC
  TCCCGCCATGGCCGCAACTTTGTGAGCTTCCGTGTTCAGGAGGCCCTTCGTGTGGCTCGC
  ACCCACCATGCCAACCATGCACTCCCAGTCTACGTCTTCACACGACCCACCTACAGCCGC
  AGGCTCACGGGGCTTAGTGAGATGGACCTCATCTCTACCATTGGCGAGAGTGCGGCCCTG
  GGCGCAGCTGGTGTCATCCTCTGGGGTGACGCGGGGTACACCACAAGCACGGAGACCTGC
  CAGTACCTCAAAGATTACCTGACACGGCTGCTGGTCCCCTACGTGGTCAATGTGTCCTGG
  GCCACCCAATATTGCAGCCGGGCCCAGTGCCATGGCCATGGGCGCTGTGTGCGCCGCAAC
  CCCAGTGCCAGTACCTTCCTGCATCTCAGCACCAACAGTTTCCGCCTAGTGCCTGGCCAT
  GCACCTGGTGAACCCCAGCTGCGACCTGTGGGGGAGCTCAGTTGGGCCGACATTGACCAC
  CTGCAGACACACTTCCGCTGCCAGTGCTACTTGGGCTGGAGTGGTGAGCAATGCCAGTGG
  GACCATAGGCAGGCAGCTGGAGGTGCCAGCGAGGCCTGGGCTGGGTCCCACCTCACCAGT
  CTGCTGGCTCTGGCAGCCCTGGCCTTTACCTGGACCTTGTAG
  
  Clone variation with respect to NM_003773.4
  52 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003773 unedited
  NGGTCGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCGAGTTCC
  TGAGCTGGTGCCAGGCAGGTGACACCTCCTGCAGCCCCCAGCATGCGGGCAGGCCCAGGC
  CCCACCGTTACATTGGCCCTGGTGCTGGCGGTGGCATGGGCCATGGAGCTCAAGCCCACA
  GCACCACCCATCTTCACTGGCCGGCCCTTTGTGGTAGCGTGGGACGTGCCCACACAGGAC
  TGTGGCCCACGCCTCAAGGTGCCACTGGACCTGAATGCCTTTGATGTGCAGGCCTCACCT
  AATGAGGGTTTTGTGAACCAGAATATTACCATCTTCTACCGCGACCGTCTAGGCCTGTAT
  CCACGCTTCGATTCTGCCGGAAGGTCTGTGCATGGTGGTGTGCCACAGAATGTCAGCCTT
  TGGGCACACCGGAAGATGCTGCAGAAACGTGTGGAGCACTACATTCGGACACAGGAGTCT
  GCGGGGCTGGCGGTCATCGACTGGGAGGACTGGCGACCTGTGTGGGTGCGCAACTGGCAG
  GACAAAGATGTGTATCGCCGGTTATCACGCCAGCTAGTGGCCAGTCGTCACCCTGACTGG
  CCTCCAGACCGCATAGTCAAACAGGCACAATATGAGNTTTGAGTTCGCAGCACAGCAGTT
  CATGCTGGAGACACTGCGTTATGTCAAGGCAGTGCGGCCCCGGCACCTCTGGGGCTTCTA
  CCTCTTTCCTGACTGCTACAATCATGNATTATGTGCAGAACTGGGAGAGCTACACAGGCC
  GCTGCCCTGATGTTTGAGGTGGCCCGCAATGACCAGCTGCCCTGGCTGTGGGCTGAGACA
  CGGNCCTCTTCCCGTCTGTCTANCTGGACGAGACACTTGCTTTCTCCGNCATGGCGCNNA
  CTTNGAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003773 unedited
  AATTTTGGNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGTTTATG
  TTTAATTTACAAAAGAGACCCCAAAATAATAATAATAATAAACTATCTAGGGCAAGGGAG
  TAGGGTCAGGTCCTCCCCAGCCCAGCTGTACCCTCCTTCCCCTCTGGCAGGGAGAGAAGG
  GGCCTCCCAGGGACTTCCCCTCCCCCTTAGAACAGGGGGGTGCGAGCTGGTATGGATGCC
  CTCCTGGGCTTCCTGGGGGCTCTGCCCACTCCAGACTCCAGTTTGTCCACCCCCTGCAGG
  GTCCTACATGCCTAAGAGAGCCCTTGTGGTAGAGGCCAGCTTGCTAGGCAGCTAGGCAGG
  AGACCCCTACAAGGTCCAGGTAAAGGCCAGGGCTGCCAGAGCCAGCAGACTGGTGAGGTG
  GGACCCAGCCCAGGCCTCGCTGGCACCTCCAGCTGCCTGCCTATGGTCCCACTGGCATTG
  CTCACCACTCCAGCCCAAGTAGCACTGGCAGCGGAAGTGTGTCTGCAGGTGGTCAATGTC
  GGCCCAACTGAGCTCCCCCACAGGTCGCAGCTGGGGTTCACCAGGTGCATGGCCAGGCAC
  TANGCGGAAACTGTTGGTGCTGAGATGCANGAAGGTACTGGCACTGGGGTTGCGGGGCAC
  ACAGCGCCCATGGCCATGGCACTGGGCCCGGGTGCAATAATGGGGTGGCCAGGGCACATT
  TGACCACCGTAGGGACCAGCAGCCGTGTCAAGTAATCTTTGAGGGCCTGGCAAGCCTCCG
  GGCTTGGGGGGACCCCCCGTAACCCCAAAGAAGACACCAGCTGGGCCCCAGGGCCGGACT
  CTCGCCCATGGTANAGAAGGAGGGCCCATCTCACTAACCCCCGGAACCTGCCGCTGTAAG
  GGGGGCCGGGGAAAACCCAAACTGGGATGCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003773
• Insert Size: 1830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003773.2, NP_003764.3
• RefSeq Size: 1848 bp
• RefSeq ORF: 1422 bp
• Locus ID: 8692
• UniProt ID: Q12891
• Cytogenetics: 3p21.31
• Domains: Glyco_hydro_56
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• Gene Summary: This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein.