HYAL2 (NM_003773) Human Tagged ORF Clone

CAT#: RC214453

HYAL2 (Myc-DDK-tagged)-Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 1

Product Images

Circular map for RC214453

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  Western blot validation of overexpression lysate (Cat# LY401242) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214453 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HYAL2
• Synonyms: LUCA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214453 representing NM_003773
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCAGGCCCAGGCCCCACCGTTACATTGGCCCTGGTGCTGGCGGTGTCATGGGCCATGGAGCTCA
  AGCCCACAGCACCACCCATCTTCACTGGCCGGCCCTTTGTGGTAGCGTGGGACGTGCCCACACAGGACTG
  TGGCCCACGCCTCAAGGTGCCACTGGACCTGAATGCCTTTGATGTGCAGGCCTCACCTAATGAGGGTTTT
  GTGAACCAGAATATTACCATCTTCTACCGCGACCGTCTAGGCCTGTATCCACGCTTCGATTCTGCCGGAA
  GGTCTGTGCATGGTGGTGTGCCACAGAATGTCAGCCTTTGGGCACACCGGAAGATGCTGCAGAAACGTGT
  GGAGCACTACATTCGGACACAGGAGTCTGCGGGGCTGGCGGTCATCGACTGGGAGGACTGGCGACCTGTG
  TGGGTGCGCAACTGGCAGGACAAAGATGTGTATCGCCGGTTATCACGCCAGCTAGTGGCCAGTCGTCACC
  CTGACTGGCCTCCAGACCGCATAGTCAAACAGGCACAATATGAGTTTGAGTTCGCAGCACAGCAGTTCAT
  GCTGGAGACACTGCGTTATGTCAAGGCAGTGCGGCCCCGGCACCTCTGGGGCTTCTACCTCTTTCCTGAC
  TGCTACAATCATGATTATGTGCAGAACTGGGAGAGCTACACAGGCCGCTGCCCTGATGTTGAGGTGGCCC
  GCAATGACCAGCTGGCCTGGCTGTGGGCTGAGAGCACGGCCCTCTTCCCGTCTGTCTACCTGGACGAGAC
  ACTTGCTTCCTCCCGCCATGGCCGCAACTTTGTGAGCTTCCGTGTTCAGGAGGCCCTTCGTGTGGCTCGC
  ACCCACCATGCCAACCATGCACTCCCAGTCTACGTCTTCACACGACCCACCTACAGCCGCAGGCTCACGG
  GGCTTAGTGAGATGGACCTCATCTCTACCATTGGCGAGAGTGCGGCCCTGGGCGCAGCTGGTGTCATCCT
  CTGGGGTGACGCGGGGTACACCACAAGCACGGAGACCTGCCAGTACCTCAAAGATTACCTGACACGGCTG
  CTGGTCCCCTACGTGGTCAATGTGTCCTGGGCCACCCAATATTGCAGCCGGGCCCAGTGCCATGGCCATG
  GGCGCTGTGTGCGCCGCAACCCCAGTGCCAGTACCTTCCTGCATCTCAGCACCAACAGTTTCCGCCTAGT
  GCCTGGCCATGCACCTGGTGAACCCCAGCTGCGACCTGTGGGGGAGCTCAGTTGGGCCGACATTGACCAC
  CTGCAGACACACTTCCGCTGCCAGTGCTACTTGGGCTGGAGTGGTGAGCAATGCCAGTGGGACCATAGGC
  AGGCAGCTGGAGGTGCCAGCGAGGCCTGGGCTGGGTCCCACCTCACCAGTCTGCTGGCTCTGGCAGCCCT
  GGCCTTTACCTGGACCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214453 representing NM_003773
  Red=Cloning site Green=Tags(s)
  
  MRAGPGPTVTLALVLAVSWAMELKPTAPPIFTGRPFVVAWDVPTQDCGPRLKVPLDLNAFDVQASPNEGF
  VNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQNVSLWAHRKMLQKRVEHYIRTQESAGLAVIDWEDWRPV
  WVRNWQDKDVYRRLSRQLVASRHPDWPPDRIVKQAQYEFEFAAQQFMLETLRYVKAVRPRHLWGFYLFPD
  CYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSRHGRNFVSFRVQEALRVAR
  THHANHALPVYVFTRPTYSRRLTGLSEMDLISTIGESAALGAAGVILWGDAGYTTSTETCQYLKDYLTRL
  LVPYVVNVSWATQYCSRAQCHGHGRCVRRNPSASTFLHLSTNSFRLVPGHAPGEPQLRPVGELSWADIDH
  LQTHFRCQCYLGWSGEQCQWDHRQAAGGASEAWAGSHLTSLLALAALAFTWTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003773
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003773.5
• RefSeq Size: 1848 bp
• RefSeq ORF: 1422 bp
• Locus ID: 8692
• UniProt ID: Q12891
• Cytogenetics: 3p21.31
• Domains: Glyco_hydro_56
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• MW: 53.7 kDa
• Gene Summary: This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010]