ST3GAL5 (NM_003896) Human Untagged Clone

CAT#: SC117667

ST3GAL5 (untagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (ST3GAL5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST3GAL5
• Synonyms: SATI; SIAT9; SIATGM3S; SPDRS; ST3Gal V; ST3GalV
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117667 sequence for NM_003896 edited (data generated by NextGen Sequencing)
  ATGCGGACGAAGGCGGCGGGCTGCGCGGAGCGGCGTCCCCTGCAGCCGCGGACCGAGGCA
  GCGGCGGCACCTGCCGGCCGAGCAATGCCAAGTGAGTACACCTATGTGAAACTGAGAAGT
  GATTGCTCGAGGCCTTCCCTGCAATGGTACACCCGAGCTCAAAGCAAGATGAGAAGGCCC
  AGCTTGTTATTAAAAGACATCCTCAAATGTACATTGCTTGTGTTTGGAGTGTGGATCCTT
  TATATCCTCAAGTTAAATTATACTACTGAAGAATGTGACATGAAAAAAATGCATTATGTG
  GACCCTGACCATGTAAAGAGAGCTCAGAAATATGCTCAGCAAGTCTTGCAGAAGGAATGT
  CGTCCCAAGTTTGCCAAGACATCAATGGCGCTGTTATTTGAGCACAGGTATAGCGTGGAC
  TTACTCCCTTTTGTGCAGAAGGCCCCCAAAGACAGTGAAGCTGAGTCCAAGTACGATCCT
  CCTTTTGGGTTCCGGAAGTTCTCCAGTAAAGTCCAGACCCTCTTGGAACTCTTGCCAGAG
  CACGACCTCCCTGAACACTTGAAAGCCAAGACCTGTCGGCGCTGTGTGGTTATTGGAAGC
  GGAGGAATACTGCACGGATTAGAACTGGGCCACACCCTGAACCAGTTCGATGTTGTGATA
  AGGTTAAACAGTGCACCAGTTGAGGGATATTCAGAACATGTTGGAAATAAAACTACTATA
  AGGATGACTTATCCAGAGGGCGCACCACTGTCTGACCTTGAATATTATTCCAATGACTTA
  TTTGTTGCTGTTTTATTTAAGAGTGTTGATTTCAACTGGCTTCAAGCAATGGTAAAAAAG
  GAAACCCTGCCATTCTGGGTACGACTCTTCTTTTGGAAGCAGGTGGCAGAAAAAATCCCA
  CTGCAGCCAAAACATTTCAGGATTTTGAATCCAGTTATCATCAAAGAGACTGCCTTTGAC
  ATCCTTCAGTACTCAGAGCCTCAGTCAAGGTTCTGGGGCCGAGATAAGAACGTCCCCACA
  ATCGGTGTCATTGCCGTTGTCTTAGCCACACATCTGTGCGATGAAGTCAGTTTGGCGGGT
  TTTGGATATGACCTCAATCAACCCAGAACACCTTTGCACTACTTCGACAGTCAATGCATG
  GCTGCTATGAACTTTCAGACCATGCATAATGTGACAACGGAAACCAAGTTCCTCTTAAAG
  CTGGTCAAAGAGGGAGTGGTGAAAGATCTCAGTGGAGGCATTGATCGTGAATTTTGA
  
  Clone variation with respect to NM_003896.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003896 unedited
  TTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCCGGCCGGCGC
  CCCCTCATTAGTATGCGGACGAAGGCGGCGGGCTGCGCGGAGCGGCGTCCCCTGCAGCCG
  CGGACCGAGGCAGCGGCGGCACCTGCCGGCCGAGCAATGCCAAGTGAGTACACCTATGTG
  AAACTGAGAAGTGATTGCTCGAGGCCTTCCCTGCAATGGTACACCCGAGCTCAAAGCAAG
  ATGAGAAGGCCCAGCTTGTTATTAAAAGACATCCTCAAATGTACATTGCTTGTGTTTGGA
  GTGTGGATCCTTTATATCCTCAAGTTAAATTATACTACTGAAGAATGTGACATGAAAAAA
  ATGCATTATGTGGACCCTGACCATGTAAAGAGAGCTCAGAAATATGCTCAGCAAGTCTTG
  CAGAAGGAATGTCGTCCCAAGTTTGCCAAGACATCAATGGCGCTGTTATTTGAGCACAGG
  TATAGCGTGGACTTACTCCCTTTTGTGCAGAAGGCCCCCAAAGACAGTGAAGCTGAGTCC
  AAGTACGATCCTCCTTTTGGGTTCCGGAAGTTCTCAGTAAAGTCCAAACCCTCTTGGAAC
  TCTTGCCAGAGCACGACCTCCCTGAACACTTTGAAACCAAGACCCTGCCGCGCTGTGTGG
  TTATTGGAAGCGGACGAATACTGCACGGATTAAAACTGGGCCCCACCCTTGAACCANTTC
  GATGTTGTGATAAGGTTAACAGTGCACCCTTTGGAGGACTTTCAAAACATGTTGGAATAA
  AACTACTATAGGTGACTTTCCCGAGGGCCCACACTTGCTTGACCTGGAATATATTCCAAT
  GACTTATTGTTGCTGGTGTATTTAAGAGGGCTATTTCACCTGGCTTCACCACGGGCAAAA
  AGGAACCCTGCCTTTGGGTACGACTCTCTTTTGGGACACGGGGGAAAAAAATCCCCGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003896 unedited
  GGCGGTGTTTCCTTTTTTATCTTTTTAAACTGGCACACTGCCTGGTATACACCGCCAGGT
  AGGCATTCAGAAAATTTCTTTTTTTTAAATACACAATTTATAATACTGGGAAGATTTCAT
  TTCAGTGTTTCCCAAAACATTATTCCTGGAAAGGGTGTACTCTCCCATGACTCTGGATAA
  TAGAAGTTTTGTTCTGATTTTTTAAGTCACCTCAGACAGACACTGGAACACGTTAGATCT
  AACACTTAAGTGCTTTGAAAGGGCAGTAAAAAATCCCCAAGGAATTCAAGAATTGTAATA
  ATTGCTGGGAAGACTGTGGTTTCTGTAGCCCAGGGTGGCTTCACAGTTGTCAGAGGTCAC
  AGATTCTATGTCCCTCTCCGACCAGGGACCTCCAGGACAGCTTCCCTGGTTGGTTCTCGA
  GTCTTTCAGCAGAAGGCAGACCAACAGAGAAGGGTTGTGACCTTCTCCAACCAGCCCAGG
  CCTCACGCCGCTGCATCGCAGACCCAGTATCAGCAGCAGAGCTACGGAGCACGTCATCCT
  GGGAGTGGATCCTCCGTGGGTCACACCAAGCAGCGCAGCAGGGAACCAGAATGAGGTTCA
  GGGCCACCATCAAAAGAGTGACCTCCCCTCTCCTTCCAATTAGTTACCTGTATTCAATGT
  GCAGTGTAAACGAGCAGAAAGTTTCAAATTAAATTACTTTCTTAAAGATCAATACAACAT
  CGTTACATACAATTCTCTNTGAAAAGTCTTTCCAATTCATTCTTAAGTTCATTTACAAAA
  TGGTGTGCAAAAACAAACACTGACCTCCAATAATATGAAAAAAAAGTGCGGAGAGCTAAA
  TTCTTTCTTGTTTTTAAATTAAAGTTAAAAACTGAGCTGGCCTCCAAGTATTTGCGGGAG
  GAGAAATACATACAGAGGCTGCCAAAACGCCTTCNAGTAAAGTGCCTTTCACCGGGGTTT
  CTGGGTCAATTACTATCAGCCTCCCTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003896
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003896.2, NP_003887.2
• RefSeq Size: 2362 bp
• RefSeq ORF: 1089 bp
• Locus ID: 8869
• UniProt ID: Q9UNP4
• Cytogenetics: 2p11.2
• Domains: Glyco_transf_29
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• Gene Summary: Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).