RHOB (NM_004040) Human Untagged Clone

CAT#: SC117625

RHOB (untagged)-Human ras homolog gene family, member B (RHOB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RHOB
• Synonyms: ARH6; ARHB; MST081; MSTP081; RHOH6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117625 sequence for NM_004040 edited (data generated by NextGen Sequencing)
  ATGGCGGCCATCCGCAAGAAGCTGGTGGTGGTGGGCGACGGCGCGTGTGGCAAGACGTGC
  CTGCTGATCGTGTTCAGTAAGGACGAGTTCCCCGAGGTGTACGTGCCCACCGTCTTCGAG
  AACTATGTGGCCGACATTGAGGTGGACGGCAAGCAGGTGGAGCTGGCGCTGTGGGACACG
  GCGGGCCAGGAGGACTACGACCGCCTGCGGCCGCTCTCCTACCCGGACACCGACGTCATT
  CTCATGTGCTTCTCGGTGGACAGCCCGGACTCGCTGGAGAACATCCCCGAGAAGTGGGTC
  CCCGAGGTGAAGCACTTCTGTCCCAATGTGCCCATCATCCTGGTGGCCAACAAAAAAGAC
  CTGCGCAGCGACGAGCATGTCCGCACAGAGCTGGCCCGCATGAAGCAGGAACCCGTGCGC
  ACGGATGACGGCCGCGCCATGGCCGTGCGCATCCAAGCCTACGACTACCTCGAGTGCTCT
  GCCAAGACCAAGGAAGGCGTGCGCGAGGTCTTCGAGACGGCCACGCGCGCCGCGCTGCAG
  AAGCGCTACGGCTCCCAGAACGGCTGCATCAACTGCTGCAAGGTGCTATGA
  
  Clone variation with respect to NM_004040.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004040 unedited
  CAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATCGGCACGAGGGCCCCGCTC
  ATGGCGGCCATCCGCAAGAAGCTGGTGGTGGTGGGCGACGGCGCGTGTGGCAAGACGTGC
  CTGCTGATCGTGTTCAGTAAGGACGAGTTCCCCGAGGTGTACGTGCCCACCGTCTTCGAG
  AACTATGTGGCCGACATTGAGGTGGACGGCAAGCAGGTGGAGCTGGCGCTGTGGGACACG
  GCGGGCCAGGAGGACTACGACCGCCTGCGGCCGCTCTCCTACCCGGACACCGACGTCATT
  CTCATGTGCTTCTCGGTGGACAGCCCGGACTCGCTGGAGAACATCCCCGAGAAGTGGGTC
  CCCGAGGTGAAGCACTTCTGTCCCAATGTGCCCATCATCCTGGTGGCCAACAAAAAAGAC
  CTGCGCAGCGACGAGCATGTCCGCACAGAGCTGGCCCGCATGAAGCAGGAACCCGTGCGC
  ACGGATGACGGCCGCGCCATGGCCGTGCGCATCCAAGCCTACGACTACCTCGAGTGCTCT
  GCCAAGACCAAGGAAGGCGTGCGCGAGGTCTTCGAGACGGCCACGCGCGCCGCGCTGCAG
  AAGCGCTACGGCTCCCAGAACGGCTGCATCAACTGCTGCAAGGTGCTATGAGGGCCGCGC
  CCGTCGCGCCTGCCCCTGCCGGCACGGCTCCCCCTCCTGGACCAGTCCCCCGCGAGCCCG
  GAGAAGGGGAGACCCGTGTCCCACAGGACCCCACCGGCCTGCCTGGCATCTGTCTGCTGA
  CGCCTCTGGCTTGCGCCAGGACTTGGCGTGGGCACCGGGCGCCCCCATCCCAGTGTCTGT
  GTGCGTCCAGCTGTGTTGCACAGGCCTGGGCTCCCACTGAGTGCCAAGGTCCCCTGAGCA
  TGCTTTCTGAGAGCCGGCCTCANATGTGTGCTGGTGTCTGTCGACTCCCTCGCCCATTTC
  ACCCACCCCGCTCTGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004040
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004040.2, NP_004031.1
• RefSeq Size: 2384 bp
• RefSeq ORF: 591 bp
• Locus ID: 388
• UniProt ID: P62745
• Cytogenetics: 2p24.1
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.[UniProtKB/Swiss-Prot Function]