Annexin VI (ANXA6) (NM_004033) Human Untagged Clone

CAT#: SC117618

ANXA6 (untagged)-Human annexin A6 (ANXA6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Annexin VI
• Synonyms: annexin A6; annexin VI; annexin VI (p68); ANX6; calcium-binding protein p68; calelectrin; calphobindin II; CBP68
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004033, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAAACCAGCACAGGGTGCCAAGTACCGGGGCTCCATCCATGACTTCCCAGGCTTTGACCCCAACC
  AGGATGCCGAGGCTCTGTACACTGCCATGAAGGGCTTTGGCAGTGACAAGGAGGCCATACTGGACATAAT
  CACCTCACGGAGCAACAGGCAGAGGCAGGAGGTCTGCCAGAGCTACAAGTCCCTCTACGGCAAGGACCTC
  ATTGCTGATTTAAAGTATGAATTGACGGGCAAGTTTGAACGGTTGATTGTGGGCCTGATGAGGCCACCTG
  CCTATTGTGATGCCAAAGAAATTAAAGATGCCATCTCGGGCATTGGCACTGATGAGAAGTGCCTCATTGA
  GATCTTGGCTTCCCGGACCAATGAGCAGATGCACCAGCTGGTGGCAGCATACAAAGATGCCTACGAGCGG
  GACCTGGAGGCTGACATCATCGGCGACACCTCTGGCCACTTCCAGAAGATGCTTGTGGTCCTGCTCCAGG
  GAACCAGGGAGGAGGATGACGTAGTGAGCGAGGACCTGGTACAACAGGATGTCCAGGACCTATACGAGGC
  AGGGGAACTGAAATGGGGAACAGATGAAGCCCAGTTCATTTACATCTTGGGAAATCGCAGCAAGCAGCAT
  CTTCGGTTGGTGTTCGATGAGTATCTGAAGACCACAGGGAAGCCGATTGAAGCCAGCATCCGAGGGGAGC
  TGTCTGGGGACTTTGAGAAGCTAATGCTGGCCGTAGTGAAGTGTATCCGGAGCACCCCGGAATATTTTGC
  TGAAAGGCTCTTCAAGGCTATGAAGGGCCTGGGGACTCGGGACAACACCCTGATCCGCATCATGGTCTCC
  CGTAGTGAGTTGGACATGCTCGACATTCGGGAGATCTTCCGGACCAAGTATGAGAAGTCCCTCTACAGCA
  TGATCAAGAATGACACCTCTGGCGAGTACAAGAAGACTCTGCTGAAGCTGTCTGGGGGAGATGATGATGC
  TGCTGGCCAGTTCTTCCCGGAGGCAGCGCAGGTGGCCTATCAGATGTGGGAACTTAGTGCAGTGGCCCGA
  GTAGAGCTGAAGGGAACTGTGCGCCCAGCCAATGACTTCAACCCTGACGCAGATGCCAAAGCGCTGCGGA
  AAGCCATGAAGGGACTCGGGACTGACGAAGACACAATCATCGATATCATCACGCACCGCAGCAATGTCCA
  GCGGCAGCAGATCCGGCAGACCTTCAAGTCTCACTTTGGCCGGGACTTAATGACTGACCTGAAGTCTGAG
  ATCTCTGGAGACCTGGCAAGGCTGATTCTGGGGCTCATGATGCCACCGGCCCATTACGATGCCAAGCAGT
  TGAAGAAGGCCATGGAGGGAGCCGGCACAGATGAAAAGGCTCTTATTGAAATCCTGGCCACTCGGACCAA
  TGCTGAAATCCGGGCCATCAATGAGGCCTATAAGGAGGACTATCACAAGTCCCTGGAGGATGCTCTGAGC
  TCAGACACATCTGGCCACTTCAGGAGGATCCTCATTTCTCTGGCCACGGGGCATCGTGAGGAGGGAGGAG
  AAAACCTGGACCAGGCACGGGAAGATGCCCAGGAAATAGCAGACACACCTAGTGGAGACAAAACTTCCTT
  GGAGACACGTTTCATGACGATCCTGTGTACCCGGAGCTATCCGCACCTCCGGAGAGTCTTCCAGGAGTTC
  ATCAAGATGACCAACTATGACGTGGAGCACACCATCAAGAAGGAGATGTCTGGGGATGTCAGGGATGCAT
  TTGTGGCCATTGTTCAAAGTGTCAAGAACAAGCCTCTCTTCTTTGCCGACAAACTTTACAAATCCATGAA
  GGGTGCTGGCACAGATGAGAAGACTCTGACCAGGATCATGGTATCCCGCAGTGAGATTGACCTGCTCAAC
  ATCCGGAGGGAATTCATTGAGAAATATGACAAGTCTCTCCACCAAGCCATTGAGGGTGACACCTCCGGAG
  ACTTCCTGAAGGCCTTGCTGGCTCTCTGTGGTGGTGAGGACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004033 unedited
  NGGTCGAATTTGTATACGACTCCTATAGGCGGCCGGCGAATCGGCACGAGGCTCCGATCC
  AGCGAGCGCTGCGTCCTCGAGTCCCTGCGCCCGTGCGTCCGTCTGCGACCCGAGGCCTCC
  GCTGCGCGTGGATTCTGCTGCGAACCGGAGACCATGGCCAAACCAGCACAGGGTGCCAAG
  TACCGGGGCTCCATCCATGACTTCCCAGGCTTTGACCCCAACCAGGATGCCGAGGCTCTG
  TACACTGCCATGAAGGGCTTTGGCAGTGACAAGGAGGCCATACTGGACATAATCACCTCA
  CGGAGCAACAGGCAGAGGCAGGAGGTCTGCCAGAGCTACAAGTCCCTCTACGGCAAGGAC
  CTCATTGCTGATTTAAAGTATGAATTGACGGGCAAGTTTGAACGGTTGATTGTGGGCCTG
  ATGAGGCCACCTGCCTATTGTGATGCCAAAGAAATTAAAGATGCCATCTCGGGCATTGGC
  ACTGATGAGAAGTGCCTCATTGAGATCTTGGCTTCCCGGACCAATGAGCAGATGCACCAG
  CTGGTGGCAGCATACAAAGATGCCTACGAGCGGGACCTGGAGGCTGACATCATCGGCGAC
  ACCTCTGGCCACTTCCAGAAGATGCTTGTGGTCCTGCTCCAGGGAACCAGGGAGGAGGAT
  GACGTAGTGAGCGAGGACCTGGTACAACAGGATGTCCAGGACCTATACGAGNGCAGGGAA
  CTGAAATGGGGAACAGATGAAGCCCAGTTCATTTACANTCTTGGGAAATCGCAGCAAGCA
  GCATCTTCGGGTTTGGTGTTCGAATGAGTATCTGAAGACCACAGGGAAGCCGATTGAAGC
  CAGCATCCGAGGGGAGCTGTCTGGNNGACTTTGAGAACTAATGCTGGNCGNNATNGAAGT
  NGTATCCGGAGCACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004033 unedited
  GGGGGGGGGGGGGCCCCNNNTTTTACTATAAAAAAAGACCTTCAGGGTTCAGATCGTTTT
  GGGTACGACTTACCCACACCAGGCANAGCTGTAGCATTGGGTAGGAAGCTGGGTAAAGCC
  TGAGGGTCAGAGGGGAGTGGAGGTGGACAGGATCTATGGCTACGGTTTTTCAGCCGCTCA
  GCCGTGCTGGGCCCTCGATGGCCCGTGGGAGTGGGAGCGTTTCCTAAGCTCCACTGAAGA
  TAAGAGCCCAACCCAACCCCTCCCCCCACCCCTGCCCCTTCCTTAGTCTCTGGAGCTGGA
  ACAATCAGGCTTGGCCATGGCGGCTGGTGCTGATAACCATTTCTTGGCAGAAGTGCCCGC
  CAAAGCTGTGGCCCTAGTCCTCACCACCACAGAGAGCCAGCAAGGCCTTCAGGAAGTCTC
  CGGAGGTGTCACCCTCAATGGCTTGGTGGAGAGACTTGTCATATTTTCTCAATGAATTCC
  CTCCGGATGTTGAGCAAGTCAATCTCACTGCGGGATACCATGATCCTGGTCAGAGTCTTC
  TCATCTGCGCCAGCACCCTTCATGGATTTGTAAAGTTTGCCCGCTAAGAAGATAGGCTTG
  TTCTCGACCCTTTCAACAATGGCCACAAATGCATACCCTGACATTTCCCAGACATCCTCT
  TCCTTGGAGGGGTGCCTCCACCTGATAATTGGCCAATCTTGTTTGTAACTTCCTGTAACG
  ACTCTTCCGAGGTGCCCGATATGCTTCCGGTTCCTCCCGATCGTCTTGAAACGTCCTCTC
  CAATGACACTCTCTCCCTCCACCTGGTTGTCCTCTGCTCCTCTTCCATCTTCTCCAATAG
  TCTCCCGTGGCTTTTTTCCCGTTCTCTTGCCTCAATGTTTTTTCCTCCCTCCCCCATTAA
  GACCCCGTGGCGCTCCGATAAATAGGTTCCCATTTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004033
• Insert Size: 2710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004033.2, NP_004024.2
• RefSeq Size: 2897 bp
• RefSeq ORF: 2004 bp
• Locus ID: 309
• Cytogenetics: 5q33.1
• Domains: annexin
• Gene Summary: Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.