CHST2 (NM_004267) Human Untagged Clone

CAT#: SC117469

CHST2 (untagged)-Human carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 (CHST2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHST2
• Synonyms: C6ST; glcNAc6ST-1; Gn6ST-1; GST-2; GST2; HEL-S-75
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004267 edited
  ATGAGCCGCAGCCCGCAGCGAGCTCTGCCCCCGGGCGCGCTCCCTCGGCTGCTCCAGGCT
  GCGCCTGCAGCCGCGCCGCGTGCCCTGCTCCCGCAGTGGCCCCGGCGCCCAGGACGCCGC
  TGGCCCGCGTCCCCTCTCGGAATGAAGGTGTTCCGTAGGAAGGCGCTGGTGTTGTGCGCG
  GGCTATGCACTGCTGCTGGTGCTCACTATGCTCAACCTCCTGGACTACAAGTGGCACAAG
  GAGCCGCTGCAGCAGTGCAACCCCGATGGGCCGCTGGGTGCCGCAGCGGGGGCAGCCGGA
  GGCAGCTGGGGGCGCCCAGGGCCGCCTCCGGCCGGGCCGCCCCGTGCTCATGCCCGTTTG
  GACCTCCGCACTCCTTACCGCCCTCCCGCTGCCGCCGTCGGGGCGGCTCCTGCAGCCGCG
  GCAGGGATGGCGGGGGTTGCGGCCCCTCCAGGCAATGGCACTCGGGGCACCGGGGGCGTC
  GGGGACAAGCGGCAGCTGGTGTACGTGTTCACCACGTGGCGCTCTGGCTCGTCGTTCTTC
  GGCGAGCTATTCAACCAGAATCCCGAGGTGTTCTTTCTCTACGAGCCAGTGTGGCATGTA
  TGGCAAAAACTGTATCCGGGGGACGCCGTTTCCCTGCAGGGGGCAGCGCGGGACATGCTG
  AGCGCTCTTTACCGCTGCGACCTCTCTGTCTTCCAGTTGTATAGCCCCGCGGGCAGCGGG
  GGGCGCAACCTCACCACGCTGGGCATCTTCGGCGCAGCCACCAACAAGGTGGTGTGCTCG
  TCACCACTCTGCCCCGCCTACCGCAAGGAGGTCGTGGGGTTGGTGGACGACCGCGTGTGC
  AAGAAGTGCCCGCCACAGCGCCTGGCGCGTTTCGAGGAGGAGTGCCGCAAGTACCGCACA
  CTAGTCATAAAGGGTGTGCGCGTCTTCGACGTGGCGGTCTTGGCGCCACTGCTGCGAGAC
  CCGGCCCTGGACCTCAAGGTCATCCACTTGGTGCGTGATCCCCGCGCGGTGGCGAGTTCA
  CGGATCCGCTCGCGCCACGGCCTCATCCGTGAGAGCCTACAGGTGGTGCGCAGCCGAGAC
  CCGCGAGCTCACCGCATGCCCTTCTTGGAGGCCGCGGGCCACAAGCTTGGCGCCAAGAAG
  GAGGGCGTGGGCGGCCCCGCAGACTACCACGCTCTGGGCGCTATGGAGGTCATCTGCAAT
  AGTATGGCTAAGACGCTGCAGACAGCCCTGCAGCCCCCTGACTGGCTGCAGGGCCACTAC
  CTGGTGGTGCGGTACGAGGACCTGGTGGGAGACCCCGTCAAGACACTACGGAGAGTGTAC
  GATTTTGTGGGACTGTTGGTGAGCCCCGAAATGGAGCAGTTTGCCCTGAACATGACCAGT
  GGCTCGGGCTCCTCCTCCAAGCCTTTCGTGGTATCTGCACGCAATGCCACGCAGGCCGCC
  AATGCCTGGCGGACCGCCCTCACCTTCCAGCAGATCAAACAGGTGGAGGAGTTTTGCTAC
  CAGCCCATGGCCGTCCTGGGCTATGAGCGGGTCAACAGCCCTGAGGAGGTCAAAGACCTC
  AGCAAGACCCTGCTTCGGAAGCCCCGTCTCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004267 unedited
  CCCAGTTGGATTTGTATACGACTCATATAGGCGGCCTGCGAATTCGCACCAGGTTTTTCT
  TCCCGAGCCGCAGGGCGCCCGCTGCCCGNGAAACTGCCCAGGGATAAGTCGGCCGACTCC
  CCAGACCCCTCGAAGGTGCGGGGACCCCCAGCGGAAGCGAGAGGGAGCGAAATCGAGGAA
  CGAGTGACAGCCGGACAGTCCGCCGGGCGGTGATCCGGGGCCGCTCCCGGGCGCGCCCTC
  GGCTCCAGGTCCTACCCGGAGCCGCTGCCATGGGAGAGCCAGCCTTGGGCGCTGGGGACC
  AGCCGCCGCGCCCGCCTCGGAGTCGCGGCCCGAGTCCCGGCGCCAGCAGCCAGCCCGCTG
  CGTCCCCTTCCCGGGCTGCAGGGCTGCCTCCGCCGCGCCGCCGGCCCGGATTGTGCCTGT
  GATGAGCCGCAGCCCGCAGCGAGCTCTGCCCCCGGGCGCGCTCCCTCGGCTGCTCCAGGC
  TGCGCCTGCAGCCGCGCCGCGTGCCCTGCTCCCGCAGTGGCCCCGGCGCCCAGGACGCCG
  CTGGCCCGCGTCCCCTCTCGGAATGAAGGTGTTCCGTAGGAAGGCGCTGGTGTTGTGCGC
  GGGCTATGCACTGCTGCTGGTGCTCACTATGCTCAACCTCCTGGACTACAAGTGGCACAA
  GGAGCCGCTGCAGCAGTGCAACCCCGATGGGCCGCTGGGTGCCGCAGCGGNGGCAGCCGG
  AGGCAGCTGGGGGCGCCCAGGGCCGCCTCCTGCCGGGCCGCCCCGTGCTCATGCCCGTTT
  GGACCTTCGCACTTCTTACCGNNCTCCCGCTGNCGCCGTCGGGGCGGCTTCTGCAGCCGC
  TGCTAGGATGGCGGGGGGTTGCGGCCTCTTCAGGCTATGGCACTCGNNG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004267 unedited
  CCAATACGTTGNCCGCGGCCGCTTCTACGTTTTTAAAAGGAAATTATTTATTAGCTTCTT
  TATTAATACTCACATGTAACCTTTGCTTTTTACACAAAAGTCTGCTTTAGAAGAATGCCT
  CCTCGGCTTATCATGCCCAATGGGGCTTTTTGTTTCTGGACCACTTCCCCTTTCTCCACC
  CCCACCCCCACATCCAAATTACTCTTAACATGTTCACAGATACCACGAATATTTTGTAAA
  CAAGATTTGGGTTACTGGAACTTGATTTCATTAACATCCCACTTCAAAATGGAAGGCAGG
  TGGAGGACAGGGTAAGAAATAGGAGAAAGAGGACAAGAGAAGGCAAAGAAGAAGAGGGGC
  ACCTGGTCAGAACGTTCAAGTCTTGCCTTTTCTTAGGGGTGTGTATTTTGTGGCTGTTCC
  TTGAAATGCACTGACTCAAAACATTGACAGCAAGTGTGTGTGTGATTATTTATATATCTA
  TAATAGAATATGTGGGCTTCCTCCGTTTGAGTCTGGACTGTGTTTATTTAAACACACTAC
  GATCCTCTTTATAGGTATCACCACAGGGAATCAGGTCTCCTGGGAACCCCTTTTAGAGAC
  GGGGCTTCCGAAGCAGGGTCTTGCTGAGGTCTTTGACCTCCTCAGGGCTGTTGACCCGCT
  CATAGCCCAGGACGGCCATGGGCTGGTAGCAAAACTCCTCCACCTGTTTGATCTGCTGGA
  AGGTGAGGGCGGTCCGCCAGGCATTGGCGGCCTGCGTGGCATTGCGTGCAGATACCACGA
  AAGGCTTGTAGGAGGAGCCCGAGCCACTGGTCATGTTCAGGGCANACTGCTCCATTTTCG
  GGCTCACCAACAGTCCCACANAATCGTACACTCTTCGTAGTGTCTTGACGGNGTCTCCCA
  CT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004267
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004267.3, NP_004258.2
• RefSeq Size: 3046 bp
• RefSeq ORF: 1593 bp
• Locus ID: 9435
• UniProt ID: Q9Y4C5
• Cytogenetics: 3q24
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Keratan sulfate biosynthesis, Metabolic pathways
• Gene Summary: This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]