PRPF4 (NM_004697) Human Untagged Clone

CAT#: SC117203

PRPF4 (untagged)-Human PRP4 pre-mRNA processing factor 4 homolog (yeast) (PRPF4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRPF4
• Synonyms: HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117203 sequence for NM_004697 edited (data generated by NextGen Sequencing)
  ATGGCTTCCTCGCGAGCCTCTTCCACGCAGGCAACCAAAACTAAAGCACCCGACGACTTA
  GTTGCTCCGGTCGTGAAGAAACCACACATCTATTATGGAAGTTTGGAAGAGAAGGAGAGG
  GAGCGTCTGGCCAAAGGAGAGTCTGGGATTTTGGGGAAAGACGGACTTAAAGCAGGGATC
  GAAGCTGGAAATATTAATATAACCTCTGGAGAAGTGTTTGAAATTGAAGAGCATATCAGC
  GAGCGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAGGAAGCGAGCCCGGCAGATCAAT
  GTTTCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAGAGCCTTGGGGGAACCCATCACA
  CTTTTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTTAAGAAATATCCTCTCAGTTGTC
  GGTACTGATGCCTTGAAAAAGACCAAAAAGGATGATGAGAAGTCTAAAAAGTCCAAAGAA
  GAGTATCAGCAAACCTGGTATCATGAAGGACCAAATAGCTTGAAGGTGGCAAGACTATGG
  ATTGCTAATTATTCGTTGCCCAGGGCAATGAAACGCTTGGAAGAGGCCCGACTCCATAAG
  GAGATTCCTGAGACAACAAGGACCTCCCAGATGCAAGAGCTGCACAAGTCTCTCCGGTCT
  TTGAATAATTTTTGCAGTCAGATTGGGGATGATCGGCCTATCTCCTACTGTCACTTTAGT
  CCCAATTCCAAGATGCTGGCCACAGCTTGTTGGAGTGGGCTTTGCAAGCTCTGGTCTGTT
  CCTGATTGCAACCTCCTTCACACTCTTCGAGGGCATAACACAAATGTAGGAGCAATTGTA
  TTCCATCCCAAATCCACTGTCTCCTTGGACCCAAAAGATGTCAACCTGGCCTCTTGTGCG
  GCTGATGGCTCTGTGAAGCTTTGGAGTCTCGACAGTGATGAACCAGTGGCAGATATTGAA
  GGCCATACAGTGCGTGTGGCGCGGGTAATGTGGCATCCTTCAGGACGTTTCCTGGGCACC
  ACCTGCTATGACCGTTCATGGCGCTTATGGGATTTGGAGGCTCAAGAGGAGATCCTGCAT
  CAGGAAGGCCATAGCATGGGTGTGTATGACATTGCCTTCCATCAAGATGGCTCTTTGGCT
  GGCACTGGGGGACTGGATGCATTTGGTCGAGTTTGGGACCTACGCACAGGACGTTGTATC
  ATGTTCTTAGAAGGCCACCTGAAAGAAATCTATGGAATAAATTTCTCCCCCAATGGCTAT
  CACATTGCAACCGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTCCGACAGCGGCGT
  TGCGTCTACACCATCCCTGCTCATCAGAACTTAGTGACTGGTGTCAAGTTTGAGCCTATC
  CATGGGAACTTCTTGCTTACTGGTGCCTATGATAACACAGCCAAGATCTGGACGCACCCA
  GGCTGGTCCCCGCTGAAGACTCTGGCTGGCCACGAAGGCAAAGTGATGGGCCTAGATATT
  TCTTCCGATGGGCAGCTCATAGCCACTTGCTCATATGACAGGACCTTCAAGCTGTGGATG
  GCTGAATAG
  
  Clone variation with respect to NM_004697.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004697 unedited
  AGAAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCTGGGC
  GCGCGGTGGACGGTCTGAAAGGGAGTGTTCGGGTTTCGCTGGGGCCTCGCGGCTCCAGAG
  CCCAGCATGGCTTCCTCGCGAGCCTCTTCCACGCAGGCAACCAAAACTAAAGCACCCGAC
  GACTTAGTTGCTCCGGTCGTGAAGAAACCACACATCTATTATGGAAGTTTGGAAGAGAAG
  GAGAGGGAGCGTCTGGCCAAAGGAGAGTCTGGGATTTTGGGGAAAGACGGACTTAAAGCA
  GGGATCGAAGCTGGAAATATTAATATAACCTCTGGAGAAGTGTTTGAAATTGAAGAGCAT
  ATCAGCGAGCGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAGGAAGCGAGCCCGGCAG
  ATCAATGTTTCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAGAGCCTTGGGGGAACCC
  ATCACACTTTTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTTAAGAAATATCCTCTCA
  GTTGTCGGTACTGATGCCTTGAAAAAGACCAAAAAGGATGATGAGAAGTCTAAAAAGTCC
  AAAGAAGAGTATCAGCAAACCTGGTATCATGAAGGACCAAATAGCTTGAAGGTGGCAAGA
  CTATNGGATTGCTAATTATTCGTTGCCCAGGGCAATGAAACGCTTGGAAGAGGCCCGACT
  CCATAAGGAGATTCCCTGAGACACAGGACCTCCCAGATGCAAGAGCTGCACAAGTCTCTC
  CGGTCTTTGAATATTNTTGCAGTCAGATGGGGGATGATCGGCCTATCTCCTACTGTCACT
  TTAGTCCCAATTCCAGATGCTGCCACAGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004697 unedited
  ACTATGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGCTTTCTAA
  ACATTTGTTTGACTCCTGACACAATGGTTTTGCTTTGGGGTAACATGAAGGTTGCCAAAC
  TTCAAAGTAAAAAAAAAGATTTTTCTGACAAAGCAAATGTTCAGTTCAGAGTTGCCTGAT
  GCTGTCAGCATTCCAAGCAAACTCAATAGGAAGAGGTGAAGTCTTCTTCACTGTATAACC
  ATCGGCGTGGCTGCGGTCTACTGCTTCACATCCCATTCTCCAGGGAAAGGAAAAGTGCTC
  GCTATGATGCATTGTTTAACCAATGAGTGGTGAGATGTGCCTCTTTACCTGAGGGCTTCC
  AGACTATAGTTCAGCCACCTTGCTCTCTGGGTCATATGTAGTCCTGAAGCATGTACCATG
  TTATGGAAAGCCAAGTTTCAAGAATTAAAAATGCAGAGTCAGTCACCATCCTGGGTGCTG
  TGAGGAAAGCTATAAGGCATTCGAACAAATTTTTTTTTTTTTTGAGACAGGGTCTTGCTC
  TGTCACACAGGCTAGAGTGCAATGGCGCAATCTCGGCTCACTGCAACCTCTGCCTCCCAA
  GCTTAAGCAATCCCTCCCACCTCAGCCTCCCAAGTAGCCGGAATCACAGGCAGAAGCCAC
  CGCGACCAGCTAATTTTAGTATTTCTTTGCAGAGACGGCATTTGCCATGTTGCCCAAGCT
  CGTCCTGAACTCCTGACCTAAGCGATCTACCCACCTCGGCCTCCCAAAATGCTAAGATTA
  CACGCGTGAGCCCCCACGCCCGGCCCAACCAAGATTTTTAAATCCTGGTTACACCGTCGT
  GGGAGGGCGTCATAAGTATTAAATCTCAACCGCGAGAGGGGCCCCCACCCCTAAGCATTG
  GCTCCCCCCGGATGGGGCCTACTCGAATCCATCCATGAGGTCTACCTGGTATTCACAAAC
  ATTTATAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004697
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004697.3, NP_004688.2
• RefSeq Size: 2756 bp
• RefSeq ORF: 1569 bp
• Locus ID: 9128
• UniProt ID: O43172
• Cytogenetics: 9q32
• Domains: WD40, SFM
• Protein Pathways: Spliceosome
• Gene Summary: The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).