SLC16A5 (NM_004695) Human Untagged Clone

CAT#: SC117201

SLC16A5 (untagged)-Human solute carrier family 16, member 5 (monocarboxylic acid transporter 6) (SLC16A5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC16A5
• Synonyms: MCT5; MCT6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117201 sequence for NM_004695 edited (data generated by NextGen Sequencing)
  ATGCCCCAGGCCCTGGAGCGTGCAGATGGCAGCTGGGCCTGGGTGGTGCTGCTGGCCACC
  ATGGTGACCCAGGGCCTCACCCTGGGCTTCCCCACGTGTATCGGCATCTTCTTCACTGAA
  TTGCAATGGGAGTTCCAGGCCAGCAACAGCGAGACCTCTTGGTTCCCCTCCATCCTCACG
  GCTGTGCTCCACATGGCAGGGCCCCTGTGCAGCATCCTGGTGGGACGCTTCGGCTGCCGA
  GTGACCGTGATGCTGGGGGGCGTGCTGGCCAGCCTGGGCATGGTGGCCAGCTCCTTCTCT
  CACAACCTCAGCCAGCTCTACTTCACAGCAGGATTCATCACAGGCCTGGGCATGTGCTTC
  AGCTTCCAGTCAAGCATCACGGTGCTGGGCTTCTACTTTGTCCGCCGGCGGGTGCTGGCC
  AACGCGCTGGCCTCGATGGGCGTCTCCCTGGGCATCACCCTCTGGCCGCTGCTCTCCCGC
  TACCTTCTGGAGAACCTGGGCTGGAGGGGTACCTTCCTTGTCTTCGGCGGGATCTTTCTC
  CACTGCTGCATCTGCGGGGCCATCATAAGGCCTGTGGCCACCAGTGTGGCCCCTGAGACC
  AAAGAATGTCCCCCGCCACCTCCCGAGACACCTGCACTTGGCTGCCTGGCTGCATGCGGC
  CGGACCATCCAGCGCCACCTGGCCTTCGACATCCTGCGGCACAACACAGGCTACTGCGTG
  TACATACTGGGTGTGATGTGGTCCGTCCTGGGCTTCCCACTGCCACAAGTCTTCCTGGTG
  CCATATGCCATGTGGCACAGCGTGGACGAGCAGCAGGCAGCCCTCCTCATCTCCATCATC
  GGCTTCAGCAACATCTTCCTGAGGCCCCTAGCCGGGCTGATGGCAGGACGGCCGGCCTTT
  GCTAGCCACCGCAAGTACCTGTTCAGCCTGGCACTCCTGCTCAATGGGCTCACTAACCTG
  GTGTGTGCGGCATCAGGTGACTTCTGGGTGCTCGTGGGCTACTGCCTGGCGTACAGCGTG
  TCCATGAGTGGCATCGGCGCCCTCATCTTCCAGGTTCTCATGGACATCGTCCCCATGGAT
  CAGTTCCCCAGAGCCCTGGGACTCTTCACTGTCCTGGACGGCCTTGCTTTCCTCATCTCC
  CCACCACTGGCCGGGTTGCTCCTGGACGCCACCAACAACTTTAGCTATGTTTTCTACATG
  TCCAGCTTCTTCCTCATCTCAGCTGCCCTCTTCATGGGTGGCAGCTTCTACGCCCTGCAG
  AAGAAGGAGCAAGGCAAGCAGGCTGTCGCGGCGGATGCCCTGGAGCGGGATCTTTTCTTG
  GAAGCCAAAGACGGTCCTGGGAAGCAACGGTCCCCTGAGATCAKGTGCCAGTCTTCCCGC
  CAGCCACGTCCAGCTGGCGTCAATAAGCATCTTTGGGGATGTCCTGCCTCCTCCAGGACC
  AGCCATGAGTGGCTCTTATGGCCAAAGGCGGTACTGCAGGCCAAGCAAACGGCTCTGGGC
  TGGAATAGCCCTACCTGA
  
  Clone variation with respect to NM_004695.2
  121 c=>t;1364 t=>k
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004695 unedited
  CCGCGGAATTCGCACGAGGCTGCCCACACATCCAGCTTCATGGCACTGTGCTACAAAGCT
  GGTCTCATTGGGCCTAGGGGTCTTTGAGAGCTGAGCTTGGCAGCCCCTGGGGCCCAACCT
  CACCCCTAGTCCCAGCAAGTGAGAGCGAAGCATCTTGCCCTGTCTACCTCCTTGCCCAAA
  GCACACACCAGGCAGTGACCATGACTCATGGTTCCCAGTACAAAGAAAAGAGCACTTCCC
  TGCTGGGGCAGAGGCCAAGCCAGAGCCCTGGCCCCATCACCTCTCTGCCCTGCTTGAGTC
  TGGAATGGACCTCCCTGCCTGGAAGCTGCATTGCCCTTGGTCCTGGGCCTGCCTGCCCCC
  TGGGGAACACAGGCAGCAGCCACATTGGCAGTGAGGCCGTGGCAGCGTCGGCAGCAGAGG
  ATGCCCCAGGCCCTGGAGCGTGCAGATGGCAGCTGGGCCTGGGTGGTGCTGCTGGCCACC
  ATGGTGACCCAGGGCCTCACCCTGNGCTTCCCCACGTGTATCGGCATCTTCTTCACTGAA
  TTGCAATGGGAGTTCCAGGCCAGCAACAGCGAGACCTCTTGGTTCCCCTCCATCCTCACG
  GCTGTGCTCCACATGGCAGGGCCCCTGTGCAGCATCCTGGTGGGACGCTTCGGCTGCCGA
  GTGACCGTGATGCTGGGGGGGCGTGCTGGCCAGCCTGGGCATGGTGGGCCAGCTCCTTCT
  CTCACAACCTCAGCCAGCTCTACTTCACAGCAGGATTCATCACAGGCCTGGGCATGTGCT
  TCAGCTTCCAGTCAAGCATCACGGTGCTGGGCTTCTACTTTGTTCGCGGCGGGTGCTGGC
  CACGCGCTGCCTCGATGGGCGTCTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004695 unedited
  GCTATGGACCGGCGGCCGCAATCTAGAGTCGAGTTNTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATAAAATTTTATTATTTGGCTCTAA
  CAAAGAAACCTCCTACTTTTTAAAGGCTAAAGAGGGGGCCAACATTTTGCCCAACTCACA
  TGGCAAATAGGGGGGGAGTCAAACAGGGCCCTCAGGGAGGGCTTTTCCACCCCAAACCCG
  TTTGCTTGGCCTGCAAAACCCCCTTTGGCCATAAAACCCACTCATGGTTGGCCCTGGAGG
  AGGCAGGACATCCCCAAAAATGCTTATTGACCCCACCTGGACGTGGTTGGCGGAAAAACT
  GGCCCCTGTTCATAAAAAAAAAATCCCCTGATAAGGTTTTTTTGCGGACCCTGGTTGACC
  CTGAAAAACAAACAACATCCTGAACCTGAGCCCCCCTTGCTGACCTAGGAGAAAAGGAAG
  GGTGAGGGAAAACGCACTCCAGCTTTGTTGGGCAGCTTCATTAGTCCACAAAGTTTATGA
  CCCTGCTGCCAGGAACATTTTTGGGTGAAATTAAAACACATTCTGTTTTATCATTCCCTC
  GGCCCCCTGCTGGGACAAAAATGGCCTGATGACACCCCCCCCCTGGGGGGTGCCTCTCGG
  GAAAGGCCCCACCAAAATTGTTGGGGGGGAACCTTGACCCCCCATCACAAGCCCCGGGTT
  TCCGGGAACCCGGGGTGAACCCACCCCCCCCTTCACCCGGGGTGGACCCAATACCACGGA
  GAGGAAAAGGGGGGCCCCTGGAGACCAAATGGGGAAAAAAAAAATTTCTTTGAAAACAAC
  CCATTTTACAAAAGTTCGGCACCCGGAAAGCCTTTTCTCCGGAAAAAAGTGGGTTCCCCC
  CGGGCCTAACTCCCCCCAGAAAAGGGGGGGAAGGAGGAAACCT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004695
• Insert Size: 4080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004695.2, NP_004686.1
• RefSeq Size: 1940 bp
• RefSeq ORF: 1518 bp
• Locus ID: 9121
• UniProt ID: O15375
• Cytogenetics: 17q25.1
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the monocarboxylate transporter family and the major facilitator superfamily. The encoded protein is localized to the cell membrane and acts as a proton-linked transporter of bumetanide. Transport by the encoded protein is inhibited by four loop diuretics, nateglinide, thiazides, probenecid, and glibenclamide. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 2 and 3 encode the same protein.