Beta 1,4 galactosyltransferase 6 (B4GALT6) (NM_004775) Human Untagged Clone

CAT#: SC117169

B4GALT6 (untagged)-Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6 (B4GALT6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Beta 1,4 galactosyltransferase 6
• Synonyms: B4Gal-T6; beta4Gal-T6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117169 sequence for NM_004775 edited (data generated by NextGen Sequencing)
  ATGTCTGTGCTCAGGCGGATGATGCGGGTTTCCAATCGCTCTCTCCTCGCCTTCATCTTC
  TTCTTCTCCCTCTCTTCGTCCTGTCTGTACTTCATCTATGTGGCCCCAGGCATCGCCAAC
  ACATATCTCTTTATGGTACAAGCTCGAGGTATAATGTTGAGAGAAAATGTGAAAACAATA
  GGTCATATGATCAGGCTGTACACAAATAAAAACAGTACGCTCAACGGTACAGATTATCCC
  GAAGGCAATAATTCAAGTGATTATCTTGTTCAAACAACAACGTATCTCCCGGAAAACTTC
  ACATACTCACCATACCTCCCCTGTCCAGAAAAGCTGCCTTATATGCGAGGATTCCTCAAT
  GTCAATGTAAGCGAAGTCAGTTTTGATGAAATTCATCAACTCTTCTCCAAGGATTTAGAT
  ATTGAGCCAGGGGGTCATTGGAGGCCAAAAGACTGTAAACCCAGATGGAAGGTGGCAGTT
  CTCATTCCTTTCCGTAATCGCCATGAACATCTTCCAATTTTTTTCTTACATCTGATTCCA
  ATGCTCCAGAAGCAGCGGCTGGAATTTGCGTTTTATGTCATTGAACAGACTGGCACACAA
  CCTTTTAACCGTGCGATGCTTTTCAATGTGGGCTTCAAAGAGGCCATGAAAGACAGTGTC
  TGGGACTGTGTAATCTTCCACGATGTGGATCATCTACCTGAAAATGACCGGAACTATTAC
  GGATGTGGAGAAATGCCACGTCATTTTGCTGCAAAGCTGGATAAATACATGTATATTCTT
  CCATATAAAGAATTTTTTGGTGGTGTAAGTGGGCTGACAGTGGAACAATTTAGAAAGATC
  AATGGTTTTCCTAATGCCTTCTGGGGATGGGGAGGAGAAGATGATGACCTTTGGAACAGA
  GTTCACTATGCTGGATATAATGTAACCAGACCAGAGGGAGACTTAGGAAAATACAAGTCA
  ATTCCTCATCACCATAGAGGTGAAGTCCAGTTTTTAGGACGGTATAAATTACTAAGGTAT
  TCCAAGGAGCGTCAGTACATCGATGGACTGAACAATTTAATATATAGGCCAAAAATACTG
  GTTGATAGGTTGTATACAAACATATCTGTAAACCTCATGCCAGAGTTAGCTCCAATCGAA
  GACTATTAA
  
  Clone variation with respect to NM_004775.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004775 unedited
  GTTCGAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGCCCTCTG
  GCCTCCCATTCATTCCGTCCGCTCCCTCCCATTTCCTCTTTTCCGCTCGGGCCCTCTCCC
  CAGAGCCTCCCCGCGCGGACCCCGCTGCAGTCTCGGGGTTCTCAGCACATTTATGGAAGT
  TTAGGGCCTGGACAGTGGCCCCGAGTCCGGCCTGAGAGCGCAGCCTGGGCTGCTGGCAGG
  GAAGAGGAAGATGTCTGTGCTCAGGCGGATGATGCGGGTTTCCAATCGCTCTCTCCTCGC
  CTTCATCTTCTTCTTCTCCCTCTCTTCGTCCTGTCTGTACTTCATCTATGTGGCCCCAGG
  CATCGCCAACACATATCTCTTTATGGTACAAGCTCGAGGTATAATGTTGAGAGAAAATGT
  GAAAACAATAGGTCATATGATCAGGCTGTACACAAATAAAAACAGTACGCTCAACGGTAC
  AGATTATCCCGAAGGCAATAATTCAAGTGATTATCTTGTTCAAACAACAACGTATCTCCC
  GGAAAACTTCACATACTCACCATACCTCCCCTGTCCAGAAAAGCTGCCTTATATGCGAGG
  ATTCCTCAATGTCAATGTAAGCGAAGTCAGTTTTGATGAAATTCATCAACTCTTCTCCAA
  GGATTTAGATATTGAGCCAGGGGGTCATTGGAGGCCAAAAGACTGTAAACCCAGATGGGA
  AGGTGGCAGTTCTCATTCCTTTCCGTAATCGCCATGAACATCTTCCAATTTTTTTCTTAC
  ATCTGATTCCAATGCTCCAGAAGCAGCGGCTGGGAAATTTGCGTTTATGTCATTGAACAG
  ACTGGCACACAACCTTTTAACCGTGCGATGCTTTTCAATGTGGGCTTNCANNAGAGCCAT
  GAAGACAGTGTCTGGGACTGTGGTATCTTCCCACGATGTGGATCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004775 unedited
  ACTATGGCCCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCATTTGCA
  ATGTATTTGTTAATCTTTATGTAATAATTAATACAATGAAGATAAACGGGCACAGCTCTG
  TCACTCTCACAGAAACATATTAAGTTCACTGTTTATAGGCAAAAATAATTTTAGGTCTAC
  ACTAAAAAAGCAAAACAAACAAACAAAAAGCAAACGAGGTGGTTTTCACAGGATGACAAA
  CTATATTTCAAAACTGAAAAAAAGCAAAATGTTTATATCTCACTCCTGAAACAAAAATTA
  ACATCAGACTTAAGAAAATAAGGCAGATACTAGTAGTACTAAGTTTTCTTGAAACTGTAA
  AATATATATAAAAATGAAAAGATACCGAATGTGGACAGCTCCACATTGATCAACAAATGT
  TAACATTCTCAATCTCTTTCATTGACTTGAAAAACTATGTAATAGAAACAGAAAATGAAC
  TAATACACAAATGAAGTACAAATATCATAATTTTCAGAAGGTTTGATTTTTCGAGTACCA
  TAAAAAAACTGAAATATAAATATTTTGGAAATAGTTCTAAGAAATAAATATGAAAATATT
  TTGGTTGGTATCATAACACAGAAGCTATCCATTTTCTCAAAGTGTAAGGATTCAATTATT
  TTATGATTTAGCTTGCGGTCATTTGTGTTATCAATATTGCCATAAAAATGACACTTCAAA
  ATAAAACTTACAATCTGAGATAAATAACCATAGCTCTTGTTTAGGTAAACAATCCTTGAG
  GCGAATATTAATCACACGGTCCTCATATATAAAACCTCTATTGCTCACCACTGGTGGTGA
  ACATTCACCTTTTACAACATCAATTTTTCGTTTAAAATTCGGAAAATTACGACATTTAAA
  CATCTTTACAATCCCAATTCACAAAACAATATGGTTCGCCTTACTGGGAAAAATATTTTC
  AAACCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004775
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004775.2, NP_004766.1
• RefSeq Size: 3830 bp
• RefSeq ORF: 1149 bp
• Locus ID: 9331
• UniProt ID: Q9UBX8
• Cytogenetics: 18q12.1
• Domains: Galactosyl_T_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes in human. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. This gene produces multiple protein isoforms - some of which are predicted to lack the N-terminal hydrophobic signal sequence and transmembrane domain. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The canonical enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis. [provided by RefSeq, Jan 2020]