POLR2D (NM_004805) Human Untagged Clone

CAT#: SC117116

POLR2D (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide D (POLR2D)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2D
• Synonyms: HSRBP4; HSRPB4; RBP4; RPB4; RPB16
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004805, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGGTGGCAGCGATCCGCGGGCTGGCGACGTAGAGGAGGACGCCTCACAGCTCATCTTTCCTA
  AAGAGTTTGAAACAGCTGAGACACTTCTAAATTCAGAAGTTCATATGCTTCTGGAACATCGAAAGCAGCA
  GAATGAGAGTGCAGAGGACGAACAGGAGCTCTCAGAAGTCTTCATGAAAACATTAAACTACACAGCCCGT
  TTCAGTCGTTTCAAAAACAGAGAGACCATTGCCAGTGTTCGTAGCTTGCTACTCCAGAAAAAGCTTCATA
  AGTTTGAGTTGGCCTGTTTGGCCAACCTTTGCCCAGAGACTGCTGAGGAGTCCAAGGCTCTAATCCCAAG
  CTTGGAGGGACGGTTTGAAGATGAGGAGCTGCAGCAGATTCTTGATGATATCCAGACAAAGCGCAGCTTT
  CAGTATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004805 unedited
  GGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGGCGCGGCG
  CGGCGATGGCGGCGGGTGGCAGCGATCCGCGGGCTGGCGACGTAGAGGAGGACGCCTCAC
  AGCTCATCTTTCCTAAAGAGTTTGAAACAGCTGAGACACTTCTAAATTCAGAAGTTCATA
  TGCTTCTGGAACATCGAAAGCAGCAGAATGAGAGTGCAGAGGACGAACAGGAGCTCTCAG
  AAGTCTTCATGAAAACATTAAACTACACAGCCCGTTTCAGTCGTTTCAAAAACAGAGAGA
  CCATTGCCAGTGTTCGTAGCTTGCTACTCCAGAAAAAGCTTCATAAGTTTGAGTTGGCCT
  GTTTGGCCAACCTTTGCCCAGAGACTGCTGAGGAGTCCAAGGCTCTAATCCCAAGCTTGG
  AGGGACGGTTTGAAGATGAGGAGCTGCAGCAGATTCTTGATGATATCCAGACAAAGCGCA
  GCTTTCAGTATTAATCTCCAAACATCACTGCTGCTCGGAGAAACCACATCCCCAGGCATA
  ACACCACCTTCCCACTGTCTGGGGCTGACTTGCACAGAAATTCTGTTGAAGACAGTTGAG
  AATTCCTTTGGAGAAAACAGCCCAGCTTGGCGTGGGGTTAGGTTGCTGTTTCAAATAACT
  CACAGGCCCAGGTGACATGGAATCTTGGAGCAGCCTTGTGCAGTGGCAGCCAGTGGCTTC
  CTGAACGTGCCTCTGCGAAGTGTGAGATGAGGGGTCACATAACCACACTGTTGACTACCT
  CATTCCTGGTTNTTGGCCTCACATCATCTTTTTTCTTATATTTCATGTTTTAATTTCAGG
  GTGTTTATACTTTTTGAAACTAGACAGNAGATAGTAGACTTATAGAGAAAGACAGTTNTA
  CCTANTACTAAAGGAGAATAAACCGCTGTAGTTGATGCTTTTTTTTTTTTTTTAATGGAA
  TAGGTCTTACTCTGTCAGCTGGAGGATGCAGTCTCAGTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004805 unedited
  TAGGACGCGGCCGCATTCTANGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTAAAAATAAAAATTTTTTATTCTTGCCTTATTTCGTAGCAAACATAAAACCATCCTGAT
  TTTAAAAGCCAACACTGTTCTTTCTCCTTTTTCCTTGGATCACAAGTTCATACAGAGTCA
  AGCAAGTGCCCCAAAAAAAAACCCCAAAATTGAAAACAAGTGTATTCCCTTCAGAAAAAT
  CAAAGCTAGAATGTTTAGCTATTGAAGTGTTTTCCCCCAGACTGAGATCAAGTGACTTCA
  CTGCAGTGGCTCAAGAGTGGATGACTCCCTCCTTCCAAATAAAGCACTTATTGCTAATTA
  GCAGGCCAGAGGCTTAGACTGGATCTCTCATAATTGGAGACTTCAAAGTGTTCAGAACTT
  CAAGCCTGGCCTATGAAGTAAAAAAAAAAAAAATTTTTTTTAAGTAGAATTGCAATTTTA
  TTTTTCTCCAGAGAAGTTTTTCTTCAGTCCTTAACAACTCCGCTCCTTATATGGGCTTTG
  GTGGAGGTCATGGGGCAGCACCTGCAGGTCTACGTTGGGGTGGAGGTGTTCGGTCCTTGC
  CTGCTTCATGAGCTCAATTCCTCACCACTGTGCTGTGAATGGCACAACTCACATGGTAAT
  GTAGCTTCACATCCAGCCTGGGAAGTACACAGGCGTCAAGGATGCTCATTTCAGAAACAT
  CGCTGACTGCTGCGGCCTCTACGTTTCAAATGATGAATTTNCTAGTAGCCTTGTCATTGG
  GCACACANCAACCACAGTTCATACAGCAAATGGGCTGCAGTGAACAGGCACACCTGGNTT
  GTGACTCTTTTGCCGACCATGCTTCTTCTTTGTATCTTGAAGTGCAGGCCCAAGAAGATG
  AGTAAAATCTTATTTGAGGTGACTAATGAATCCTTCAGACACTTACCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004805
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004805.2, NP_004796.1
• RefSeq Size: 1909 bp
• RefSeq ORF: 429 bp
• Locus ID: 5433
• UniProt ID: O15514
• Cytogenetics: 2q14.3
• Domains: RNA_pol_Rpb4, RPOL4c
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]