NDUFS1 (NM_005006) Human Untagged Clone

CAT#: SC117000

NDUFS1 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFS1
• Synonyms: CI-75k; CI-75Kd; MC1DN5; PRO1304
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005006, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTAAGGATACCTGTAAGAAAGGCCTTAGTAGGCCTTTCTAAGTCTCCTAAAGGATGTGTTCGAACAA
  CTGCCACAGCAGCAAGCAACTTGATTGAAGTATTTGTTGATGGTCAGTCTGTCATGGTGGAACCGGGAAC
  GACCGTCCTCCAAGCTTGTGAGAAGGTTGGCATGCAGATCCCTCGATTCTGTTATCATGAAAGGTTGTCT
  GTTGCTGGAAACTGCAGGATGTGCCTTGTTGAAATTGAGAAAGCCCCTAAGGTTGTAGCTGCTTGTGCCA
  TGCCAGTAATGAAGGGTTGGAATATCCTAACAAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGAT
  GGAGTTCTTATTAGCAAATCACCCATTGGACTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTGCAG
  GACCAGTCCATGATGTTTGGAAATGATAGGAGCCGATTTTTAGAGGGGAAGCGTGCTGTGGAAGACAAGA
  ACATTGGGCCATTGGTAAAGACCATCATGACAAGATGTATACAGTGTACTCGCTGCATCAGGTTTGCAAG
  TGAGATTGCAGGAGTAGATGATTTGGGAACAACAGGCAGAGGAAATGATATGCAAGTTGGCACATACATT
  GAAAAGATGTTCATGTCTGAACTGTCTGGGAATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTCTA
  AGCCCTATGCCTTTACTGCCCGGCCTTGGGAAACAAGAAAGACAGAATCCATTGATGTAATGGATGCGGT
  TGGAAGTAATATTGTGGTTAGCACAAGAACTGGAGAAGTGATGAGGATTTTGCCACGTATGCATGAGGAC
  ATCAATGAAGAGTGGATCTCTGATAAAACCAGATTTGCCTATGATGGGCTAAAACGTCAAAGACTTACCG
  AGCCAATGGTCAGAAATGAAAAAGGGCTTTTAACCTATACTTCTTGGGAGGATGCGCTCTCTCGCGTAGC
  TGGAATGTTGCAGAGTTTTCAAGGCAAAGATGTGGCAGCAATTGCAGGTGGCTTGGTGGATGCTGAAGCC
  CTGGTAGCTCTCAAAGATTTGCTTAATAGAGTGGACTCTGACACCTTATGCACTGAAGAGGTCTTCCCCA
  CTGCAGGAGCTGGCACAGATTTGCGTTCCAATTATCTTCTTAATACTACAATTGCTGGTGTGGAAGAGGC
  AGATGTTGTTCTTCTGGTTGGTACAAACCCACGTTTTGAGGCACCACTGTTTAATGCTAGAATTCGAAAG
  AGCTGGCTGCATAATGACTTAAAAGTGGCCCTTATAGGCAGTCCAGTGGACCTCACTTACACATATGACC
  ACCTGGGAGACTCCCCCAAAATTCTTCAAGACATTGCTTCGGGAAGCCATCCATTTAGCCAGGTCCTAAA
  GGAAGCTAAAAAACCAATGGTGGTTTTAGGCAGTTCTGCACTCCAAAGAAATGATGGAGCAGCAATTCTT
  GCAGCTGTTTCTAGCATTGCACAAAAGATTCGGATGACTAGTGGTGTTACTGGTGATTGGAAAGTTATGA
  ATATCCTTCATAGGATTGCAAGTCAAGTAGCTGCTTTGGACCTTGGCTATAAGCCTGGGGTGGAAGCAAT
  TCGGAAGAACCCTCCCAAGGTGCTGTTTCTCCTGGGAGCAGATGGAGGTTGTATCACACGACAGGATTTG
  CCAAAGGATTGTTTCATTATTTATCAAGGACATCATGGTGATGTTGGGGCTCCCATAGCTGATGTTATTC
  TCCCAGGAGCTGCTTACACAGAGAAGTCTGCTACATATGTCAACACTGAGGGTAGAGCTCAGCAGACTAA
  GGTAGCAGTGACACCTCCTGGCTTGGCAAGAGAAGACTGGAAAATTATAAGAGCACTCTCTGAGATTGCT
  GGAATGACTCTTCCATATGATACTCTGGATCAAGTAAGGAACAGATTGGAAGAAGTCTCTCCTAATCTTG
  TTCGATATGATGATATTGAAGGGGCTAATTACTTCCAGCAAGCAAATGAGCTCTCAAAGCTAGTGAACCA
  GCAGCTTCTTGCTGACCCACTTGTTCCACCTCAGCTAACTATAAAAGACTTCTACATGACAGATTCAATT
  AGCAGAGCCTCACAGACAATGGCCAAATGTGTCAAAGCTGTCACAGAGGGTGCCCAGGCAGTAGAGGAAC
  CATCCATATGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005006 unedited
  AAGTCACAAATTGTATCCGACTCATATAGGCGGCCGCGTAATTCGCACGAGCGGCCTCCT
  AGGGGTCGTCGTGGTCCAGACAGTTTAGCAGAACAGCCTCCGCGGCATCCGGGGCAGAAG
  CAATATGTTAAGGATACCTGTAAGAAAGGCCTTAGTAGGCCTTTCTAAGTCTCCTAAAGG
  ATGTGTTCGAACAACTGCCACAGCAGCAAGCAACTTGATTGAAGTATTTGTTGATGGTCA
  GTCTGTCATGGTGGAACCGGGAACGACCGTCCTCCAAGCTTGTGAGAAGGTTGGCATGCA
  GATCCCTCGATTCTGTTATCATGAAAGGTTGTCTGTTGCTGGAAACTGCAGGATGTGCCT
  TGTTGAAATTGAGAACGCCCCTAAGGTTGTAGCTGCTTGTGCCATGCCAGTAATGAAGGG
  TTGGAATATCCTAACAAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGATGGAGTT
  CTTATTAGCAAATCACCCATTGGACTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCT
  GCAGGACCAGTCCATGATGTTTGGAAATGATAGGAGCCGATTTTTAGAGGGGAAGCGTGC
  TGTGGAAGACAAGAACATTGGGCCATTGGTAAAGACCATCATGACAAGATGTATACAGTG
  TACTCCCTGCATCAGGTTTGCAAGTGAGATTGCAGGAGTAGATGATTTGGGAACAACAGG
  CAGACTGAAAGATATGCAAGTTGCCACATACATTGAAAAGATCTTCATGTCTGAACTGCC
  TCGGCAATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTTTAAGCCCTATGCCTTTA
  CTGGCCTGGCCTTGGGAAACCAGCAAGACCACATCCATTGATTGTCATGGATGCGGTTGG
  CAAGCAAACTGTGGTTAACCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005006 unedited
  TCCAGGCCCGGTAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGGAT
  CTCTTTATGTTCGTCACAAAGGTTATCAATGCTTTTAAGAGCATCTGCATAGTTTTGTTA
  TTTAACCTTTACACACAATACATGTTTTTCAAACTGCAAAGTTGATAACTAATATCATTT
  TCAAATAGGCATAGTATAACCTTAAATATTACATGATTCAAATTATTATTATTTTTTTTT
  ACAAAGAAATAAACCTGTAAAGGATCACTGCACTACAGTTGTCCATTAATTATCTGCGGC
  AAAACTGGGATCCTAGTAGAAGCTTCAGCATATGGATGGTTCCTCTACTGCCTGGGCACC
  CTCTGTGACAGCTTTGACACATTTGGCCATTGTCTGTGAGGCTCTGCTAATTGAATCTGT
  CATGTAGAAGTCTTTTATAGTTAGCTGAGGTGGAACAAGTGGGTCAGCAAGAAGCTGCTG
  GTTCACTAGCTTTGAGAGCTCATTTGCTTGCTGGAAGTAATTAGCCCCTTCATATCATCA
  TATCGAACAAGATTAGGAGAGACTTCTTCCAATCTGTTCCTTACTTGATCCAGAGTATCA
  TATGGAAGAGTCATTCCAGNCATCTCAGAGAGTGCTCTTATNNATTTCCAGTCTTCTCTT
  GCCAGCCCAGNAGNGTCACTGCTACCTTAGTCTGCTGAGCTCTACCCTCAGTGTGACATA
  TGTANCAGACTTCTCTGTGGTAGCAGCTCTGGGAGAATACTCAGCTATGGGAGCCCACAT
  ACCATGATGTCCTGATAATATGAAACANTCTTNGCACATCTGTCTGTGAACAACCTCTCT
  GCTCAGGAGAAAAGACCTGTGGGAGGTCTTTCAAATGCTTCCCCCAGCTTATACCAGGGT
  CAGCANNTCTGACTGCATCCTGAAGAATTCTACTTCAACCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005006
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005006.5, NP_004997.4
• RefSeq Size: 3417 bp
• RefSeq ORF: 2184 bp
• Locus ID: 4719
• UniProt ID: P28331
• Cytogenetics: 2q33.3
• Domains: fer2, molybdopterin
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (1) represents the longest and predominant transcript. Variant 1 encodes isoform 1.