beta 2 Adrenergic Receptor (ADRB2) (NM_000024) Human Untagged Clone

CAT#: SC116997

ADRB2 (untagged)-Human adrenergic, beta-2-, receptor, surface (ADRB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADRB2
• Synonyms: ADRB2R; ADRBR; B2AR; BAR; BETA2AR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000024 edited
  GAATTCGGCACGAGGCAGGCACCGCGAGCCCCTAGCACCCGACAAGCTGAGTGTGCAGGA
  CGAGTCCCCACCACACCCACACCACAGCCGCTGAATGAGGCTTCCAGGCGTCCGCTCGCG
  GCCCGCAGAGCCCCGCCGTGGGTCCGCCTGCTGAGGCGCCCCCAGCCAGTGCGCTTACCT
  GCCAGACTGCGCGCCATGGGGCAACCCGGGAACGGCAGCGCCTTCTTGCTGGCACCCAAT
  GGAAGCCATGCGCCGGACCACGACGTCACGCAGCAAAGGGACGAGGTGTGGGTGGTGGGC
  ATGGGCATCGTCATGTCTCTCATCGTCCTGGCCATCGTGTTTGGCAATGTGCTGGTCATC
  ACAGCCATTGCCAAGTTCGAGCGTCTGCAGACGGTCACCAACTACTTCATCACTTCACTG
  GCCTGTGCTGATCTGGTCATGGGCCTAGCAGTGGTGCCCTTTGGGGCCGCCCATATTCTT
  ATGAAAATGTGGACTTTTGGCAACTTCTGGTGCGAGTTTTGGACTTCCATTGATGTGCTG
  TGCGTCACGGCCAGCATTGAGACCCTGTGCGTGATCGCAGTGGATCGCTACTTTGCCATT
  ACTTCACCTTTCAAGTACCAGAGCCTGCTGACCAAGAATAAGGCCCGGGTGATCATTCTG
  ATGGTGTGGATTGTGTCAGGCCTTACCTCCTTCTTGCCCATTCAGATGCACTGGTACAGG
  GCCACCCACCAGGAAGCCATCAACTGCTATGCCAATGAGACCTGCTGTGACTTCTTCACG
  AACCAAGCCTATGCCATTGCCTCTTCCATCGTGTCCTTCTACGTTCCCCTGGTGATCATG
  GTCTTCGTCTACTCCAGGGTCTTTCAGGAGGCCAAAAGGCAGCTCCAGAAGATTGACAAA
  TCTGAGGGCCGCTTCCATGTCCAGAACCTTAGCCAGGTGGAGCAGGATGGGCGGACGGGG
  CATGGACTCCGCAGATCTTCCAAGTTCTGCTTGAAGGAGCACAAAGCCCTCAAGACGTTA
  GGCATCATCATGGGCACTTTCACCCTCTGCTGGCTGCCCTTCTTCATCGTTAACATTGTG
  CATGTGATCCAGGATAACCTCATCCGTAAGGAAGTTTACATCCTCCTAAATTGGATAGGC
  TATGTCAATTCTGGTTTCAATCCCCTTATCTACTGCCGGAGCCCAGATTTCAGGATTGCC
  TTCCAGGAGCTTCTGTGCCTGCGCAGGTCTTCTTTGAAGGCCTATGGCAATGGCTACTCC
  AGCAACGGCAACACAGGGGAGCAGAGTGGATATCACGTGGAACAGGAGAAAGAAAATAAA
  CTGCTGTGTGAAGACCTCCCAGGCACGGAAGACTTTGTGGGCCATCAAGGTACTGTGCCT
  AGCGATAACATTGATTCACAAGGGAGGAATTGTAGTACAAATGACTCACTGCTATAAAGC
  AGTTTTTCTACTTTTAAAGACCCCCCCCCGCCCAACAGAACACTAAACAGACTATTTAAC
  TTGAGGGTAATAAACTTAGAATAAAATTGTAAAATTGTATAGAGATATGCAGAAGGAAGG
  GCATCCTTCTGCCTTTTTTATTTTTTTAAGCTGTAAAAAGAGAGAAAACTTATTTGAGTG
  ATTATTTGTTATTTGTACAGTTCAGTTCCTCTTTGCATGGAATTTGTAAGTTTATGTCTA
  AAGAGCTTTAGTCCTAGAGGACCTGAGTCTGCTATATTTTCATGACTTTTCCATGTATCT
  ACCTCACTATTCAAGTATTAGGGGTAATATATTGCTGCTGGTAATTTGTATCTGAAGGAG
  ATTTTCCTTCCTACACCCTTGGACTTGAGGATTTTGAGTATCTCGGACCTTTCAGCTGTG
  AACATGGACTCTTCCCCCACTCCTCTTATTTGCTCACACGGGGTATTTTAGGCAGGGATT
  TGAGGAGCAGCTTCAGTTGTTTTCCCGAGCAAAGTXXXXXXXXXXXXXXXAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000024 unedited
  GCGGTCGAGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCAGGCAC
  CGCGAGCCCCTAGCACCCGACAAGCTGAGTGTGCAGGACGAGTCCCCACCACACCCACAC
  CACAGCCGCTGAATGAGGCTTCCAGGCGTCCGCTCGCGGCCCGCAGAGCCCCGCCGTGGG
  TCCGCCTGCTGAGGCGCCCCCAGCCAGTGCGCTTACCTGCCAGACTGCGCGCCATGGGGC
  AACCCGGGAACGGCAGCGCCTTCTTGCTGGCACCCAATGGAAGCCATGCGCCGGACCACG
  ACGTCACGCAGCAAAGGGACGAGGTGTGGGTGGTGGGCATGGGCATCGTCATGTCTCTCA
  TCGTCCTGGCCATCGTGTTTGGCAATGTGCTGGTCATCACAGCCATTGCCAAGTTCGAGC
  GTCTGCAGACGGTCACCAACTACTTCATCACTTCACTGGCCTGTGCTGATCTGGTCATGG
  GCCTAGCAGTGGTGCCCTTTGGGGCCGCCCATATTCTTATGAAAATGTGGACTTTTGGCA
  ACTTCTGGTGCGAGTTTTGGACTTCCATTGATGTGCTGTGCGTCACGGCCAGCATTGAGA
  CCCTGTGCGTGATCGCAGTGGATCGCTACTTTGCCATTACTTCACCTTTCAAGTACCAGA
  GCCTGCTGACCAAGAATAAGGCCCGGGTGATCATTCTGATGGTGTGGATTGTGTCAGGCC
  TTACCTCCTTCTTGCCCATTCAGATGCACTGGTACAGGGCCACCCACCAGGAAGCCATCA
  ACTGCTATGCCAATGAGACCTGCTGTGACTTCTTCACGAACCAAGCCTATGCCATTGCCT
  CTTCCATCGTGTCCTTCTACGTTCCCCCTGGTGATCATGGTNCTTCGTCTACTCCAGGGT
  C
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000024 unedited
  ACCTTCAGACATCTTATTACTGTAAACTTTATACTTTGCTCGGGAAACAACTGAAGCTGC
  TCCTCAAATCCCTGCCTAAAATACCCCGTGTGAGCAAATAAGAGGAGTGGGGGAAGAGTC
  CATGTTCACAGCTGAAAGGTCCGAGATACTCAAAATCCTCAAGTCCAAGGGTGTAGGAAG
  GAAAATCTCCTTCAGATACAAATTACCAGCAGCAATATATTACCCCTAATACTTGAATAG
  TGAGGTAGATACATGGAAAAGTCATGAAAATATAGCAGACTCAGGTCCTCTAGGACTAAA
  GCTCTTTAGACATAAACTTACAAATTCCATGCAAAGAGGAACTGAACTGTACAAATAACA
  AATAATCACTCAAATAAGTTTTCTCTCTTTTTACAGCTTAAAAAAATAAAAAAGGCAGAA
  GGATGCCCTTCCTTCTGCATATCTCTATACAATTTTACAATTTTATTCTAAGTTTATTAC
  CCTCAAGTTAAATAGTCTGTTTAGTGTTCTGTTGGGCGGGGGGGGGTCTTTAAAAGTAGA
  AAAACTGCTTTATAGCAGTGAGTCATTTGTACTACAATTCCTCCCTTGTGAATCAATGTT
  ATCGCTAGGCACAGTACCTTGATGGCCCACAAAGTCTTCCGTGCCTGGGAAGTCTTCACA
  CAGCAGTTTATTTTCTTTCTCCTGTTCCACGTGATATCCACTCTGCTCCCCTGTGTTGCC
  GTTGCTGGAGTAGCCATTGCCATANGCCTTCAAAGAGACCTGCGCAGCACAGAAGCTCCT
  GGAAGGCATCCTNGAATCTGGGCTCCNGCAGTAGATAAGGGGATTGAAACCAGAATTGAC
  ATAGCCTATCCAATTGAGAAGATGTAAACTTCCNTACGATGANGTATCCTGGATCAATGC
  ACATGTAACGATGAAGAAGGCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000024
• Insert Size: 2050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000024.3, NP_000015.1
• RefSeq Size: 2015 bp
• RefSeq ORF: 1242 bp
• Locus ID: 154
• UniProt ID: P07550
• Cytogenetics: 5q32
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Endocytosis, Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This receptor is also a transcription regulator of the alpha-synuclein gene, and together, both genes are believed to be associated with risk of Parkinson's Disease. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity, type 2 diabetes and cardiovascular disease. [provided by RefSeq, Oct 2019]