MMP9 (NM_004994) Human Untagged Clone

CAT#: SC116989

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MMP9 (untagged)-Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9)



  "NM_004994" in other vectors (6)

Reconstitution Protocol

USD 972.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MMP9
Synonyms CLG4B; GELB; MANDP2; MMP-9
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_004994 edited
GAATTCGGCACGAGGATGAGCCTCTGGCAGCCCCTGGTCCTGGTGCTCCTGGTGCTGGGC
TGCTGCTTTGCTGCCCCCAGACAGCGCCAGTCCACCCTTGTGCTCTTCCCTGGAGACCTG
AGAACCAATCTCACCGACAGGCAGCTGGCAGAGGAATACCTGTACCGCTATGGTTACACT
CGGGTGGCAGAGATGCGTGGAGAGTCGAAATCTCTGGGGCCTGCGCTGCTGCTTCTCCAG
AAGCAACTGTCCCTGCCCGAGACCGGTGAGCTGGATAGCGCCACGCTGAAGGCCATGCGA
ACCCCACGGTGCGGGGTCCCAGACCTGGGCAGATTCCAAACCTTTGAGGGCGACCTCAAG
TGGCACCACCACAACATCACCTATTGGATCCAAAACTACTCGGAAGACTTGCCGCGGGCG
GTGATTGACGACGCCTTTGCCCGCGCCTTCGCACTGTGGAGCGCGGTGACGCCGCTCACC
TTCACTCGCGTGTACAGCCGGGACGCAGACATCGTCATCCAGTTTGGTGTCGCGGAGCAC
GGAGACGGGTATCCCTTCGACGGGAAGGACGGGCTCCTGGCACACGCCTTTCCTCCTGGC
CCCGGCATTCAGGGAGACGCCCATTTCGACGATGACGAGTTGTGGTCCCTGGGCAAGGGC
GTCGTGGTTCCAACTCGGTTTGGAAACGCAGATGGCGCGGCCTGCCACTTCCCCTTCATC
TTCGAGGGCCGCTCCTACTCTGCCTGCACCACCGACGGTCGCTCCGACGGCTTGCCCTGG
TGCAGTACCACGGCCAACTACGACACCGACGACCGGTTTGGCTTCTGCCCCAGCGAGAGA
CTCTACACCCGGGACGGCAATGCTGATGGGAAACCCTGCCAGTTTCCATTCATCTTCCAA
GGCCAATCCTACTCCGCCTGCACCACGGACGGTCGCTCCGACGGCTACCGCTGGTGCGCC
ACCACCGCCAACTACGACCGGGACAAGCTCTTCGGCTTCTGCCCGACCCGAGCTGACTCG
ACGGTGATGGGGGGCAACTCGGCGGGGGAGCTGTGCGTCTTCCCCTTCACTTTCCTGGGT
AAGGAGTACTCGACCTGTACCAGCGAGGGCCGCGGAGATGGGCGCCTCTGGTGCGCTACC
ACCTCGAACTTTGACAGCGACAAGAAGTGGGGCTTCTGCCCGGACCAAGGATACAGTTTG
TTCCTCGTGGCGGCGCATGAGTTCGGCCACGCGCTGGGCTTAGATCATTCCTCAGTGCCG
GAGGCGCTCATGTACCCTATGTACCGCTTCACTGAGGGGCCCCCCTTGCATAAGGACGAC
GTGAATGGCATCCGGCACCTCTATGGTCCTCGCCCTGAACCTGAGCCACGGCCTCCAACC
ACCACCACACCGCAGCCCACGGCTCCCCCGACGGTCTGCCCCACCGGACCCCCCACTGTC
CACCCCTCAGAGCGCCCCACAGCTGGCCCCACAGGTCCCCCCTCAGCTGGCCCCACAGGT
CCCCCCACTGCTGGCCCTTCTACGGCCACTACTGTGCCTTTGAGTCCGGTGGACGATGCC
TGCAACGTGAACATCTTCGACGCCATCGCGGAGATTGGGAACCAGCTGTATTTGTTCAAG
GATGGGAAGTACTGGCGATTCTCTGAGGGCAGGGGGAGCCGGCCGCAGGGCCCCTTCCTT
ATCGCCGACAAGTGGCCCGCGCTGCCCCGCAAGCTGGACTCGGTCTTTGAGGAGCCGCTC
TCCAAGAAGCTTTTCTTCTTCTCTGGGCGCCAGGTGTGGGTGTACACAGGCGCGTCGGTG
CTGGGCCCGAGGCGTCTGGACAAGCTGGGCCTGGGAGCCGACGTGGCCCAGGTGACCGGG
GCCCTCCGGAGTGGCAGGGGGAAGATGCTGCTGTTCAGCGGGCGGCGCCTCTGGAGGTTC
GACGTGAAGGCGCAGATGGTGGATCCCCGGAGCGCCAGCGAGGTGGACCGGATGTTCCCC
GGGGTGCCTTTGGACACGCACGACGTCTTCCAGTACCGAGAGAAAGCCTATTTCTGCCAG
GACCGCTTCTACTGGCGCGTGAGTTCCCGGAGTGAGTTGAACCAGGTGGACCAAGTGGGC
TACGTGACCTATGACATCCTGCAGTGCCCTGAGGACTAGGGCTCCCGTCCTGCTTTGGCA
GTGCCATGTAAATCCCCACTGGGACCAACCCTGGGGAAGGAGCCAGTTTGCCGGATACAA
ACTGGTATTCTGTTCTGGAGGAAAGGGAGGAGTGGAGGTGGGCTGGGCCCTCTCTTCTCA
CCTTTGTTTTTTGTTGGAGTGTTTCTAATAAACTTGGATTCTCTAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_004994 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGAGCCTCTGGCAGCC
CCTGGTCCTGGTGCTCCTGGTGCTGGGCTGCTGCTTTGCTGCCCCCAGACAGCGCCAGTC
CACCCTTGTGCTCTTCCCTGGAGACCTGAGAACCAATCTCACCGACAGGCAGCTGGCAGA
GGAATACCTGTACCGCTATGGTTACACTCGGGTGGCAGAGATGCGTGGAGAGTCGAAATC
TCTGGGGCCTGCGCTGCTGCTTCTCCAGAAGCAACTGTCCCTGCCCGAGACCGGTGAGCT
GGATAGCGCCACGCTGAAGGCCATGCGAACCCCACGGTGCGGGGTCCCAGACCTGGGCAG
ATTCCAAACCTTTGAGGGCGACCTCAAGTGGCACCACCACAACATCACCTATTGGATCCA
AAACTACTCGGAAGACTTGCCGCGGGCGGTGATTGACGACGCCTTTGCCCGCGCCTTCGC
ACTGTGGAGCGCGGTGACGCCGCTCACCTTCACTCGCGTGTACAGCCGGGACGCAGACAT
CGTCATCCAGTTTGGTGTCGCGGAGCACGGAGACGGGTATCCCTTCGACGGGAAGGACGG
GCTCCTGGCACACGCCTTTCCTCCTGGCCCCGGCATTCAGGGAGACGCCCATTTCGACGA
TGACGAGTTGTGGTCCCTGGGCAAGGGCGTCGTGGTTCCAACTCGGTTTGGAAACGCAGA
TGGCGCGGNCTGCCACTTCCCCTTCATCTTCGNAGGCCGCTCCTACTCTGCCTGCACCAC
CGACGGTCGCTCCGACGGCTTGCCCTGGTGCAGTACCACGGCCAACTACGACACCGACGA
CCGGNTTGGCTTNCTGCCCAGCGAGAGACTCTACACCCGGGACNGGCATGCTGATGGGAA
ACCCTGNCAGTTNCATTCATCTTCAGGNCCATACTACTNCGCTGCCCN
>OriGene 3' read for NM_004994 unedited
TTTTTTTTTTTNNTNTNNTNNTTTTTTTNTTTTTTGTNNTTTTTTTTTTTTCGATTTCTT
CAGCAACACTTCCACAAAAACAAGGTGTACAACACGGGCCCAGTCCACTCCCTCCTCCTT
TCCTCCAGAACAGATACCAGTTTGTATCCGGCAAACTGGCTCCTTCCCCAGGTTGGTCCC
AGCGGGGATCCACATGGCACTGCCAACCCACGACGGCCCCCCCTAGTCCTCAGGGCACTG
CCGGCACGTCACAGGTCCACGTCCCCCACTTGGCCCACCTGGTACAACTCACTTCGGGAA
CTCACGCTTCATTCTTAATCCGTTCTCGCCCCAAATAGGCTCTCTCCTTCCTCCTGCTAC
CCGTCCCCGCGTGCCCACACGCTCCCCGGTTGACCTTCCTGTCCCCCCTCCTGGCCCTCC
CGGTGATTTACCCTCCTCTGCCTCCACTCCCTCACCTCCATAGGTCCTCGTTCTCTCACC
ATCTCCTCTCTCCCCCTGCCCCCTCCCCAGGGTCACCTCCCCCCTGCGCCCACGTCCGCC
TCCCCATGCCTCTTTTTGTTCACACCCCTCGCTTTCGATTTATTGCCGCACCCTGAGTAC
ACCCCTCACCCCAGCTGCCCATCTTCACCCATCTAATCCTTCCCTGTCACGCCGTCCCCC
CCCCCCACCGCACCTCCCCGCCTGCCACCGCCCCTGCCGCCCCCACCCCTCCCCCCCAAC
CCCAAAAGCGCCCCCTGCCATCTTGGCCTCCCTCCTTCTCTTTATCAAACAACACAAACT
CTGCCCTTCCCTCGTAAAATTTACTATTGCCACCCCCACCCCCCCCGTATGACACGCATA
CCATGTTCATATTCCGCCCATATTTCACCCTATGAACTTACATTGTCCACTTCACGGGCC
CGCATTATGGGCTCACCCTCTTGCGCCCCCCCCCTGTCTTCACTCCTCCCCGCCCTCCCC
CACCTCTTCCCTCCCTCCCCCGCCCCCCTCCACCG
Restriction Sites NotI-NotI     
ACCN NM_004994
Insert Size 2500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004994.2, NP_004985.2
RefSeq Size 2387 bp
RefSeq ORF 2124 bp
Locus ID 4318
UniProt ID P14780
Cytogenetics 20q13.12
Domains FN2, hemopexin, Peptidase_M10, ZnMc, PT
Protein Families Druggable Genome, Protease
Protein Pathways Bladder cancer, Leukocyte transendothelial migration, Pathways in cancer
Gene Summary Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

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