PIP5K2 alpha (PIP4K2A) (NM_005028) Human Untagged Clone

CAT#: SC116981

PIP4K2A (untagged)-Human phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (PIP4K2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIP5K2 alpha
• Synonyms: PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116981 sequence for NM_005028 edited (data generated by NextGen Sequencing)
  ATGGCGACCCCCGGCAACCTAGGGTCCTCTGTCCTGGCGAGCAAGACCAAGACCAAGAAG
  AAGCACTTCGTAGCGCAGAAAGTGAAGCTGTTTCGGGCCAGCGACCCGCTGCTCAGCGTC
  CTCATGTGGGGGGTAAACCACTCGATCAATGAACTGAGCCATGTTCAAATCCCTGTTATG
  TTGATGCCAGATGACTTCAAAGCCTATTCAAAAATAAAGGTGGACAATCACCTTTTTAAC
  AAAGAAAACATGCCGAGCCATTTCAAGTTTAAGGAATACTGCCCGATGGTCTTCCGTAAC
  CTGCGGGAGAGGTTTGGAATTGATGATCAAGATTTCCAGAATTCCCTGACCAGGAGCGCA
  CCCCTCCCCAACGACTCCCAGGCCCGCAGTGGAGCTCGTTTTCACACTTCCTACGACAAA
  AGATACATCATCAAGACTATTACCAGTGAAGACGTGGCCGAAATGCACAACATCCTGAAG
  AAATACCACCAGTACATAGTGGAATGTCATGGGATCACCCTTCTTCCCCAGTTCTTGGGC
  ATGTACCGGCTTAATGTTGATGGAGTTGAAATATATGTGATAGTTACAAGAAATGTATTC
  AGCCACCGTTTGTCTGTGTATAGGAAATACGACTTAAAGGGCTCTACAGTGGCTAGAGAA
  GCTAGTGACAAAGAAAAGGCCAAAGAACTGCCAACTCTGAAAGATAATGATTTCATTAAT
  GAGGGCCAAAAGATTTATATTGATGACAACAACAAGAAGGTCTTCCTGGAAAAACTAAAA
  AAGGATGTTGAGTTTCTGGCCCAGCTGAAGCTCATGGACTACAGTCTGCTGGTGGGAATT
  CATGATGTGGAGAGAGCCGAACAGGAGGAAGTGGAGTGTGAGGAGAACGATGGGGAGGAG
  GAGGGCGAGAGCGATGGCACCCACCCGGTGGGAACCCCCCCAGATAGCCCCGGGAATACA
  CTGAACAGCTCACCACCCCTGGCTCCCGGGGAGTTCGATCCGAACATCGACGTCTATGGA
  ATTAAGTGCCATGAAAACTCGCCTAGGAAGGAGGTGTACTTCATGGCAATTATTGACATC
  CTTACTCATTATGATGCAAAAAAGAAAGCTGCCCATGCTGCAAAAACTGTTAAACATGGC
  GCTGGCGCGGAGATCTCCACCGTGAACCCAGAACAGTATTCAAAGCGCTTTTTGGACTTT
  ATTGGCCACATCTTGACGTAA
  
  Clone variation with respect to NM_005028.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005028 unedited
  AGTATTTTGTAATACGACTCACTATAGGGCGGCCGCGACTTCGGCACGAGTATATAGGCG
  GGTCCCCGGCCTCGGGAGCACGGCGGTGGAGGGGACATAGGAGGCGGCCATGGCGACCCC
  CGGCAACCTATGGTCCTCTGTCCTGGCGAGCAAGACCAAGACCAAGAAGAAGCACTTCGT
  AGCGCATAAAGTGAAGCTGTTTCGGGCCAGCGACCCGCTGCTCATCGTCCTCATGTGGGG
  GGTAAACCACTCGATCAATGAACTGAGCCATGTTCAAATCCCTGTTATGTTGATGCCAGA
  TGACTTCAAAGCCTATTCAAAAATAAAGGTGGACAATCACCTTTTTAACAAAGAAAACAT
  GCCGAGCCATTTCAAGTTTAAGGAATACTGCCCGATGGTCTTCCGTAACCTGCGGGAGAG
  GTTTGGAATTGATGATCAAGATTTCCAGAATTCCCTGACCAGGAGCGCACCCCTCCCCAA
  CGACTCCCAGGCCCGCAGTGGAGCTCGTTTTCACACTTCCTACGACAAAAGATACATCAT
  CAAGACTATTACCAGTGAAGACGTGGCCGAAATGCACAACATCCTGAAGAAATACCACCA
  GTACATAGTGGAATGTCATGGGATCACCCTTCTTCCCCAGTTCTTGAGCATGTACCGGCT
  TAATGTTGATGGAGTTGAAATATATGTGATAGTTACAAGATATGTATTCAGCCACCGTTT
  GTCTGTGTATATGAAATACGACTTATAGGGCTCTACAGTGGCTAGAGAAGCTAGTGACAT
  AGATAAGGCCAAAGAACTGCCCACTCTGNNAAGATATGATCTCATGAATGAGGGCCANNA
  GATTATATTGATGACCACACAAGAAGGTCTTCTGGGAAACTANAAAGGATGTGAGTTTCT
  GGCCCACTGAGCTCATGACTACATCTGCTGTGGGGATTCTGATGTGGANAGAGCAACAGN
  AGNATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005028
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005028.3, NP_005019.2
• RefSeq Size: 1781 bp
• RefSeq ORF: 1221 bp
• Locus ID: 5305
• UniProt ID: P48426
• Cytogenetics: 10p12.2
• Domains: PIP5K
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• Gene Summary: Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).