H10 (H1F0) (NM_005318) Human Untagged Clone

CAT#: SC116822

H1F0 (untagged)-Human H1 histone family, member 0 (H1F0)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H10
• Synonyms: H1.0; H1F0; H1FV; H10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116822 sequence for NM_005318 edited (data generated by NextGen Sequencing)
  ATGACCGAGAATTCCACGTCCGCCCCTGCGGCCAAGCCCAAGCGGGCCAAGGCCTCCAAG
  AAGTCCACAGACCACCCCAAGTATTCAGACATGATCGTGGCTGCCATCCAGGCCGAGAAG
  AACCGCGCTGGCTCCTCGCGCCAGTCCATTCAGAAGTATATCAAGAGCCACTACAAGGTG
  GGTGAGAACGCTGACTCGCAGATCAAGTTGTCCATCAAGCGCCTGGTCACCACCGGTGTC
  CTCAAGCAGACCAAAGGGGTGGGGGCCTCGGGGTCCTTCCGGCTAGCCAAGAGCGACGAA
  CCCAAGAAGTCAGTGGCCTTCAAGAAGACCAAGAAGGAAATCAAGAAGGTAGCCACGCCA
  AAGAAGGCATCCAAGCCCAAGAAGGCTGCCTCCAAAGCCCCAACCAAGAAACCCAAAGCC
  ACCCCGGTCAAGAAGGCCAAGAAGAAGCTGGCTGCCACGCCCAAGAAAGCCAAAAAACCC
  AAGACTGTCAAAGCCAAGCCGGTCAAGGCATCCAAGCCCAAAAAGGCCAAACCAGTGAAA
  CCCAAAGCAAAGTCCAGTGCCAAGAGGGCCGGCAAGAAGAAGTGA
  
  Clone variation with respect to NM_005318.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005318 unedited
  GTCATAATTGTATACGACTCCTATAGGCGGCCGGAATCGCCGAGGGAAAGGGAGGCACTA
  GAGGCGGAGGCAGAGGCAGAGGCAGAGCCCGGTGCCGAGACCAAGCGACAGACCGGCGGG
  GCTGGGCCTCGCAAAGCCGGCTCGGCGAGCTCTCCCGACACCCGAGCCGGGGAGGAAAAG
  CAGCGACTCCTCGCTCGCATCCCCGGGAGCCGCACTCCAGACTGGCCCGGTAGTCAGGGG
  CTCAGGAGCAGATCCCGAGGCAGGCTTTGCTCAGCCTCCGACGAGGGCTGGCCCTTTGGA
  AGGCGCCTTCAACAGCCGGACCAGACAGGCCACCATGACCGAGAATTCCACGTCCGCCCC
  TGCGGCCAAGCCCAAGCGGGCCAAGGCCTCCAAGAAGTCCACAGACCACCCCAAGTATTC
  AGACATGATCGTGGCTGCCATCCAGGCCGAGAAGAACCGCGCTGGCTCCTCGCGCCAGTC
  CATTCAGAAGTATATCAAGAGCCACTACAAGGTGGGTGAGAACGCTGACTCGCAGATCAA
  GTTGTCCATCAAGCGCCTGGTCACCACCGGTGTCCTCAAGCAGACCAAAGGGGTGGGGGC
  CTCGGNGTCCTTCCGGCTAGCCAAGAGCGACGAACCCAAGAAGTCAGTGGCCTTCAAGAA
  GACCAAGAAGGAAATCAAGAAGGTAGCCACGCCAAGAAGGCATNNCAGCCCAAGAGGCTG
  CCTCCAAGCCCCACCAAGAAACCCAAGCCACCCGGTCAAGAAGCCAAGAAGAGCTGGCCT
  GCACGCCAAGAAGCCAAAAAACCCAGACTGTCAAGCCCAGCCCGGTCAGGCATCCAGCCC
  CANAAGGCAAACCAGTTGAACCCAAGCAAGTTCAGTGCCAAGAGGTCCGCCAGAAGAAGT
  GACATGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005318 unedited
  AATTATTTAAGTTTTAAAATTACAACTGGTTAAGGAAAAAGAATTATTCTGTAATTTCTG
  AAGAATCCAAATCCTGTCTCTTATAAAGTCAGAAACAGAAGGGGGCAAAAGGTGGTGGTG
  AGCATCAAGAGAGAAAAAAGGAGAAAATTATTTACAGAAAATAGGAGACAGGAGGGAGTG
  TCCGCAAGAAAAGACTTCATTGTCACTTCTTCTTGCCGGCCCTCTTGGCACTGGACTTTG
  CTTTGGGTTTCACTGGTTTGGCCTTTTTGGGCTTGGATGCCTTGACCGGCTTGGCTTTGA
  CAGTCTTGGGTTTTTTGGCTTTCTTGGGCGTGGCAGCCAGCTTCTTCTTGGCCTTCTTGA
  CCGGGGTGGCTTTGGGTTTCTTGGTTGGGGCTTTGGAGGCAGCCTTCTTGGGCTTGGATG
  CCTTCTTTGGCGTGGCTACCTTCTTGATTTCCTTCTTGGTCTTCTTGAAGGCCACTGACT
  TCTTGGGTTCGTCGCTCTTGGCTAGCCGGAAGGACCCCGAGGCCCCCACCCCTTTGGTCT
  GCTTGAGGACACCGGTGGTGACCAGGCGCTTGATGGACAACTTGATCTGCGAGTCAGCGT
  TCTCACCCACCTTGTAGTGGCTCTTGATATACTTCTGAATGGACTGGCGCGAGGAGCCAG
  CGCGGTTCTTCTCGGCCTGGATGGCAGCCACGATCATGTCTGAAATACTTGGGGTGGNTC
  TGTGGACTTCTTGGAAGGCCTTGCNCCGCTTGGGCTTGGCCGCAGGGCCGGACGTGGAAA
  TTCTCGTCATGGTGGNCTGTCTGGTCCNGGCTGTGAAGGCGCTTNCAAAGGCCAGNNCTC
  GTCGAGCTAGCAAANCTGCTTGGAATCTGTCCTGGACCCTGACTACGGCAGTCTGAATGC
  GGTTCCGGAATGCAGCGAGGATCCTGCTTTTCTTCCGTTCGGGGTCGGGAACTCCCGAAC
  CGCTTTGAGGCCAACCCCCGGTTTTTTTTGGTTTNCAGGTTTTTCTTGCCTTTCTCCCCT
  TTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005318
• Insert Size: 1210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005318.2, NP_005309.1
• RefSeq Size: 2258 bp
• RefSeq ORF: 585 bp
• Locus ID: 3005
• UniProt ID: P07305
• Cytogenetics: 22q13.1
• Domains: linker_histone
• Protein Families: Druggable Genome
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015]