H10 (H1F0) (NM_005318) Human Tagged ORF Clone

CAT#: RC201643

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H1F0 (Myc-DDK-tagged)-Human H1 histone family, member 0 (H1F0)



  "NM_005318" in other vectors (4)

Reconstitution Protocol

USD 300.00

USD 450.00

In Stock*

Size
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol H1F0
Synonyms H1.0; H1F0; H1FV; H10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201643 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGAGAATTCCACGTCCGCCCCTGCGGCCAAGCCCAAGCGGGCCAAGGCCTCCAAGAAGTCCACAG
ACCACCCCAAGTATTCAGACATGATCGTGGCTGCCATCCAGGCCGAGAAGAACCGCGCTGGCTCCTCGCG
CCAGTCCATTCAGAAGTATATCAAGAGCCACTACAAGGTGGGTGAGAACGCTGACTCGCAGATCAAGTTG
TCCATCAAGCGCCTGGTCACCACCGGTGTCCTCAAGCAGACCAAAGGGGTGGGGGCCTCGGGGTCCTTCC
GGCTAGCCAAGAGCGACGAACCCAAGAAGTCAGTGGCCTTCAAGAAGACCAAGAAGGAAATCAAGAAGGT
AGCCACGCCAAAGAAGGCATCCAAGCCCAAGAAGGCTGCCTCCAAAGCCCCAACCAAGAAACCCAAAGCC
ACCCCGGTCAAGAAGGCCAAGAAGAAGCTGGCTGCCACGCCCAAGAAAGCCAAAAAACCCAAGACTGTCA
AAGCCAAGCCGGTCAAGGCATCCAAGCCCAAAAAGGCCAAACCAGTGAAACCCAAAGCAAAGTCCAGTGC
CAAGAGGGCCGGCAAGAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201643 protein sequence
Red=Cloning site Green=Tags(s)

MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKL
SIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKA
TPVKKAKKKLAATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005318
ORF Size 582 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005318.4
RefSeq Size 2336 bp
RefSeq ORF 585 bp
Locus ID 3005
UniProt ID P07305
Cytogenetics 22q13.1
Domains linker_histone
Protein Families Druggable Genome
MW 20.9 kDa
Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015]

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