GPR35 (NM_005301) Human Untagged Clone

CAT#: SC116808

GPR35 (untagged)-Human G protein-coupled receptor 35 (GPR35), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR35
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005301 edited
  TTTGGCAGCGGKGGTCACACACTCCACCCGGGAGGCCAAAGCTGCCTGCAGGACCATGAA
  TGGCACCTACAACACCTGTGGCTCCAGCGACCTCACCTGGCCCCCAGCGATCAAGCTGGG
  CTTCTACGCCTACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAACAGCCTGGCGCT
  CTGGGTGTTCTGCTGCCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAACCT
  GGCGGTGGCCGACCTCTGCCTGCTGTGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGA
  CACCTCAGACACGCCGCTGTGCCAGCTCTCCCAGGGCATCTACCTGACCAACAGGTACAT
  GAGCATCAGCCTGGTCACGGCCATCGCCGTGGACCGCTATGTGGCCGTGCGGCACCCGCT
  GCGTGCCCGCGGGCTGCGGTCCCCCAGGCAGGCTGCGGCCGTGTGCGCGGTCCTCTGGGT
  GCTGGTCATCGGCTCCCTGGTGGCTCGCTGGCTCCTGGGGATTCAGGAGGGCGGCTTCTG
  CTTCAGGAGCACCCGGCACAATTTCAACTCCATGGCGTTCCCGCTGCTGGGATTCTACCT
  GCCCCTGGCCGTGGTGGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCCCAGAGGCC
  ACCCACCGACGTGGGGCAGGCAGAGGCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAA
  CCTCCTGGTGTTCGTGGTCTGCTTCCTGCCCCTGCACGTGGGGCTGACAGTGCGCCTCGC
  AGTGGGCTGGAACGCCTGTGCCCTCCTGGAGACGATCCGTCGCGCCCTGTACATAACCAG
  CAAGCTCTCAGATGCCAACTGCTGCCTGGACGCCATCTGCTACTACTACATGGCCAAGGA
  GTTCCAGGAGGCGTCTGCACTGGCCGTGGCTCCCAGTGCTAAGGCCCACAAAAGCCAGGA
  CTCTCTGTGCGTGACCCTCGCCTAAGAGGCGTGCTGTGGGCGCTGTGGGCCAGGTCTCGG
  GGGCTCCGGGAGGTGCTGCCTGCCAGGGGAAGCTGGAACCAGTAGCAAGGAGCCCGRGAT
  CAGCCCTGAACTCACTGTGTATTCTCTTGGAGCCTTGGGTGGGCAGGGACGGCCCAGGTA
  CCTGCTCTCTTGGGAAGAGAGAGGGACAGGGACAAGGGCAAGAGGACTGAGGCCAGAGCA
  AGGCCAATGTCAGAGACCCCCGGGATGGGGCCTCACACTTGCCACCCCCAGAACCAGCTC
  ACCTGGCCAGAGTGGGTTCCTGCTGGCCAGGGTGCAGCCTTGATGACACCTGCCGCTGCC
  CCTCGGGGCTGGAATAAAACTCCCCACCCAGAGTCAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005301 unedited
  NATTTTGTNATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTTTGGCAGCGG
  GGGTCACACACTCCACCCGGGAGGCCAAAGCTGCCTGCAGGACCATGAATGGCACCTACA
  ACACCTGTGGCTCCAGCGACCTCACCTGGCCCCCAGCGATCAAGCTGGGCTTCTACGCCT
  ACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAACAGCCTGGCGCTCTGGGTGTTCT
  GCTGCCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAACCTGGCGGTGGCCG
  ACCTCTGCCTGCTGTGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGACACCTCAGACA
  CGCCGCTGTGCCAGCTCTCCCAGGGCATCTACCTGACCAACAGGTACATGAGCATCAGCC
  TGGTCACGGCCATCGCCGTGGACCGCTATGTGGCCGTGCGGCACCCGCTGCGTGCCCGCG
  GGCTGCGGTCCCCCAGGCAGGCTGCGGCCGTGTGCGCGGTCCTCTGGGTGCTGGTCATCG
  GCTCCCTGGTGGCTCGCTGGCTCCTGGGGATTCAGGAGGGCGGCTTCTGCTTCAGGAGCA
  CCCGGCACAATTTCAACTCCATGGCGTTCCCGCTGCTGGGATTCTACCTGCCCCTGGCCG
  TGGTGGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCCCAGAGGCCACCCACCGACG
  TGGGGCAGGCAGAGGCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAACCTCCTGGNTG
  TTCGTGGTCTGCTTCCTGCCCCTGCACGTGGNGCTGACAGTGCGCCTCGCAGTGGGCTGG
  AACGCCCTGTGCCTCCTGGAGACGATCCGTCGCGCCTGTACATACCAGCAGCTCTCAGAT
  GCACTGCTGCTGNACGCATCTGCTCTACTACTGGNNCAGAGTNCAGNNAGCGCTGACTGG
  CCGTGCTCCAGTGCTAAGCCACAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_005301 unedited
  NTTCGTGGGNCCGCGGCCGTTTTCTATTATCGAGTTTTTTTTTTTTTTTTTTTTTTTTCT
  TTGACTCTGGGTGGGGAGACCCAATCCAGCCCCGAGGGGCAGCGGCACCNCCNCCNCCGG
  CATTTTTTTTTGTTTNNTGAANTACTCTGGCCAGGCGAACTGGTTCCGGGGGTGCCTCTT
  GTGCCGTCCCCTCCCCCCGGCCCTTCAATTGGCCTTTTTCNTCCCCTCCCCCTTCCTCCC
  TGCTCCTGCCCCTCCTCTCTTCCCAAGAGAGCAGGTACCTGGGCCGTCCCTGCCCACCCA
  AGGGTCCAAGAGAATACACAGCGAGTTCAGGGCTGATCTCGGGCTTCTTGCTACTGGTTC
  CAGCTTCCCCTGGCAGGCAGGACCTCCCGGAGCCCCTGAGACCTGGCCCACAGCGCCCAC
  AGTACGCCTCTTATGCGAGGGGCACGCACAGAGAGTCCTGGCTTTTGTGGGCCTTATCAC
  TGGGAGCCACGGGCAGTGGAGAAGCCTTCTGGAACTCCTTGGCCATGTTCTTATACCAGA
  TGGCGGCCAGGCACCATTCGGCCTCCGAGAAGCTGCCGGTTATGTTCAGGGCGCGACTGA
  CTGTCTCCACGACGGCACAGGTGGTTCAGCCCATTGGCAGGGGTACTGTCCGCCACACGT
  GCATGGGCAGTGAGAAATCCCGACATCAAGAGGGGTGGCCAACACAAGCGGGCAGTCTTG
  CGGGGGTCTCTGTCCGCCCCAGTGTGGGGTGGGCTTTGGGCAAGGAGTCCCCACCTTTAG
  GGACAACAGCCACCCCGGCAGGGCAGGTAAAACTCACCGGGGAACGCCTGGAATGTAATT
  GGCCGGGTGCTTCTGAGCAAAAACGCCTTTCTGTATCCCAGAACCACGAAGCCCCCAGGA
  ACCGTGACCATCACCTACGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005301
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005301.2, NP_005292.2
• RefSeq Size: 1875 bp
• RefSeq ORF: 930 bp
• Locus ID: 2859
• UniProt ID: Q9HC97
• Cytogenetics: 2q37.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the shorter isoform (a, also known as GPR35a).