AMPK beta 2 (PRKAB2) (NM_005399) Human Untagged Clone

CAT#: SC116791

PRKAB2 (untagged)-Human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMPK beta 2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005399 edited
  ATTCGGCACCAGCTTTATTGGTGGCCTGATCTGCAGAACAGCCATCACATCAGTGGCCCT
  TGGAGGAGGGAGCGCATCGCCCGAGGTGGTCCCCGACGAGCTGCAGCCATGGGAAACACC
  ACCAGCGACCGGGTGTCCGGGGAGCGCCACGGCGCCAAGGCTGCACGCTCCGAGGGCGCA
  GGCGGCCATGCCCCGGGGAAGGAGCACAAGATCATGGTGGGGAGTACGGACGACCCCAGC
  GTGTTCAGCCTCCCTGACTCCAAGCTCCCTGGGGACAAAGAGTTTGTATCATGGCAGCAG
  GATTTGGAGGACTCCGTAAAGCCCACACAGCAGGCCCGGCCCACTGTTATCCGCTGGTCT
  GAAGGAGGCAAGGAGGTCTTCATCTCTGGGTCCTTCAACAATTGGAGCACCAAGATTCCA
  CTGATTAAGAGCCATAATGACTTTGTTGCCATCCTGGACCTCCCTGAGGGAGAGCACCAA
  TACAAGTTCTTTGTGGATGGACAGTGGGTTCATGATCCATCAGAGCCTGTGGTTACCAGT
  CAGCTTGGCACAATTAACAATTTGATCCATGTCAAGAAATCTGATTTTGAGGTGTTCGAT
  GCTTTAAAGTTAGATTCTATGGAAAGTTCTGAGACATCTTGTAGAGACCTTTCCAGCTCA
  CCCCCAGGGCCTTATGGTCAAGAAATGTATGCGTTTCGATCTGAGGAAAGATTCAAATCC
  CCACCCATCCTTCCTCCTCATCTACTTCAAGTTATTCTTAACAAAGACACTAATATTTCT
  TGTGACCCAGCCTTACTCCCTGAGCCCAACCATGTTATGCTGAACCATCTCTATGCATTG
  TCCATTAAGGACAGTGTGATGGTCCTTAGCGCAACCCATCGCTACAAGAAGAAGTATGTT
  ACTACTCTGCTATACAAGCCCATTTGAAGGGATCCCTTCTTGCCTCTAAGGATTCAGGAG
  AAGCATCTCCCTTGCATTTCTGGACTGAACCAGTCTTACCTGAGACTGGAAGGCTGATTT
  GCTTTGAGGCTGATATGTGTGTTTCAGAGCCTCTGAGTAGGATGCTCTGCTTTTGCATTT
  GATTGCAGATGAGAGCTTTATGAGTTCACGGAATTTATTTTAAGAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005399 unedited
  TGCAGAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACCAGCTTTATT
  GGTGGCCTGATCTGCAGAACAGCCATCACATCAGTGGCCCTTGGAGGAGGGAGCGCATCG
  CCCGAGGTGGTCCCCGACGAGCTGCAGCCATGGGAAACACCACCAGCGACCGGGTGTCCG
  GGGAGCGCCACGGCGCCAAGGCTGCACGCTCCGAGGGCGCAGGCGGCCATGCCCCGGGGA
  AGGAGCACAAGATCATGGTGGGGAGTACGGACGACCCCAGCGTGTTCAGCCTCCCTGACT
  CCAAGCTCCCTGGGGACAAAGAGTTTGTATCATGGCAGCAGGATTTGGAGGACTCCGTAA
  AGCCCACACAGCAGGCCCGGCCCACTGTTATCCGCTGGTCTGAAGGAGGCAAGGAGGTCT
  TCATCTCTGGGTCCTTCAACAATTGGAGCACCAAGATTCCACTGATTAAGAGCCATAATG
  ACTTTGTTGCCATCCTGGACCTCCCTGAGGGAGAGCACCAATACAAGTTCTTTGTGGATG
  GACAGTGGGTTCATGATCCATCAGAGCCTGTGGTTACCAGTCAGCTTGGCACAATTAACA
  ATTTGATCCATGTCAAGAAATCTGATTTTGAGGTGTTCGATGCTTTAAAGTTAGATTCTA
  TGGAAAGTTCTGAGACATCTTGTAGAGACCTTTCCAGCTCACCCCCAGGGCCTTATGGTC
  AAGAAATGTATGCGTTTCGATCTGAGGAAAGATTCAAATNCCCACCCATCCTTTCTNCTC
  ATCTACTTCAAGTTATTCTTAACAAAACACTAATTTNTCTTGTGACCCAGCCTTACTCCC
  TGAGCCCAAACCATGTATGCTGAACCATCTCTATGCATTGTCCATTAGGNACAGTGTGAT
  GGTTCTTTAGCGCACCATCGCTACAGAAGAAGTATGTACTACTCTGCTTACAAGCCATTN
  GAAGGATCCCTTCTTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005399 unedited
  NNCCGGTTTATCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTT
  TGCTGTATAATACTTTTATTAAAGTTTTATGGGAAAAAACCCACAACATNTTCTGTATGT
  ATCATATCATATAATTTCCAGGTGTGTCAGAGTCCAGTGACAAATTACGTTTGAAGAACA
  GCTTTAAAATCCACTCTTGTCTGCCACTCCTTTATGAAATATTGAAAAGCAGCTCTGTAC
  CTCAGCATTAAAGGTTACAAAAAGCACCATTAAAAAGAGGACTCACATATTTAATTCCCT
  TCAAAGTAGTCTTATCTCTCCTTTCTGACAGACACAGAGCTGCACTCATTCGAAATGCTG
  CCGTGTAATACCAACCAACATGCTCCAATGTACCAAAATTCAAACAAGTCTCATTTATGA
  GACCCGTGTAGTGATTAGGACTAACCAACAACAGTGAGCTTTGGCTGTCCTAAAACCACA
  AGCTGGGTTAGGTAAGTGTTTTGTACAGGGGAACAGAAAAGAAGTAAGATCATCAATACC
  TAATATATGACTTAATGTAGGTATCCCTTGACCATTTAACCTGACACCTACAAAATCCAT
  AGTGTTTAGTATTTCACACCAATCTTACTGCAAAAGTGCTGTGCTACATACAATCAATTC
  AAGTTAACTCCAACTAATATTGGAACTAATAAAAAATTAATTTGTAACAAAGCTTATTCA
  CTTCAAAGCACAACTGAGAAAAGCAAATCCAGACAAAGCCCAAACTAGAATCCTAGAGCG
  ATTCCATTGCTAAGAATTGGACTCTACAAGTAATAGACTTGAGTTNTTCTCTTTTTTCCT
  TCCTTCTTTAATTTAATTTTGCTTTTACTCTAAAGTTTGGGTTCATGCAGAATCCAGTCC
  TTCTATCCCATGTGATTGCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005399
• Insert Size: 5270 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005399.3, NP_005390.1
• RefSeq Size: 5431 bp
• RefSeq ORF: 819 bp
• Locus ID: 5565
• UniProt ID: O43741
• Cytogenetics: 1q21.1
• Domains: AMPKBI
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
• Gene Summary: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes a functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.