HSP70-1B (HSPA1B) (NM_005346) Human Untagged Clone

CAT#: SC116767

HSPA1B (untagged)-Human heat shock 70kDa protein 1B (HSPA1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSP70-1B
• Synonyms: HSP70-1; HSP70-1B; HSP70-2; HSP70.1; HSP70.2; HSP72; HSPA1; HSX70
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005346, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAAAGCCGCGGCGATCGGCATCGACCTGGGCACCACCTACTCCTGCGTGGGGGTGTTCCAACACG
  GCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACCACCCCCAGCTACGTGGCCTTCACGGACAC
  CGAGCGGCTCATCGGGGATGCGGCCAAGAACCAGGTGGCGCTGAACCCGCAGAACACCGTGTTTGACGCG
  AAGCGGCTGATCGGCCGCAAGTTCGGCGACCCGGTGGTGCAGTCGGACATGAAGCACTGGCCTTTCCAGG
  TGATCAACGACGGAGACAAGCCCAAGGTGCAGGTGAGCTACAAGGGGGAGACCAAGGCATTCTACCCCGA
  GGAGATCTCGTCCATGGTGCTGACCAAGATGAAGGAGATCGCCGAGGCGTACCTGGGCTACCCGGTGACC
  AACGCGGTGATCACCGTGCCGGCCTACTTCAACGACTCGCAGCGCCAGGCCACCAAGGATGCGGGTGTGA
  TCGCGGGGCTCAACGTGCTGCGGATCATCAACGAGCCCACGGCCGCCGCCATCGCCTACGGCCTGGACAG
  AACGGGCAAGGGGGAGCGCAACGTGCTCATCTTTGACCTGGGCGGGGGCACCTTCGACGTGTCCATCCTG
  ACGATCGACGACGGCATCTTCGAGGTGAAGGCCACGGCCGGGGACACCCACCTGGGTGGGGAGGACTTTG
  ACAACAGGCTGGTGAACCACTTCGTGGAGGAGTTCAAGAGAAAACACAAGAAGGACATCAGCCAGAACAA
  GCGAGCCGTGAGGCGGCTGCGCACCGCCTGCGAGAGGGCCAAGAGGACCCTGTCGTCCAGCACCCAGGCC
  AGCCTGGAGATCGACTCCCTGTTTGAGGGCATCGACTTCTACACGTCCATCACCAGGGCGAGGTTCGAGG
  AGCTGTGCTCCGACCTGTTCCGAAGCACCCTGGAGCCCGTGGAGAAGGCTCTGCGCGACGCCAAGCTGGA
  CAAGGCCCAGATTCACGACCTGGTCCTGGTCGGGGGCTCCACCCGCATCCCCAAGGTGCAGAAGCTGCTG
  CAGGACTTCTTCAACGGGCGCGACCTGAACAAGAGCATCAACCCCGACGAGGCTGTGGCCTACGGGGCGG
  CGGTGCAGGCGGCCATCCTGATGGGGGACAAGTCCGAGAACGTGCAGGACCTGCTGCTGCTGGACGTGGC
  TCCCCTGTCGCTGGGGCTGGAGACGGCCGGAGGCGTGATGACTGCCCTGATCAAGCGCAACTCCACCATC
  CCCACCAAGCAGACGCAGATCTTCACCACCTACTCCGACAACCAACCCGGGGTGCTGATCCAGGTGTACG
  AGGGCGAGAGGGCCATGACGAAAGACAACAATCTGTTGGGGCGCTTCGAGCTGAGCGGCATCCCTCCGGC
  CCCCAGGGGCGTGCCCCAGATCGAGGTGACCTTCGACATCGATGCCAACGGCATCCTGAACGTCACGGCC
  ACGGACAAGAGCACCGGCAAGGCCAACAAGATCACCATCACCAACGACAAGGGCCGCCTGAGCAAGGAGG
  AGATCGAGCGCATGGTGCAGGAGGCGGAGAAGTACAAAGCGGAGGACGAGGTGCAGCGCGAGAGGGTGTC
  AGCCAAGAACGCCCTGGAGTCCTACGCCTTCAACATGAAGAGCGCCGTGGAGGATGAGGGGCTCAAGGGC
  AAGATCAGCGAGGCGGACAAGAAGAAGGTTCTGGACAAGTGTCAAGAGGTCATCTCGTGGCTGGACGCCA
  ACACCTTGGCCGAGAAGGACGAGTTTGAGCACAAGAGGAAGGAGCTGGAGCAGGTGTGTAACCCCATCAT
  CAGCGGACTGTACCAGGGTGCCGGTGGTCCCGGGCCTGGCGGCTTCGGGGCTCAGGGTCCCAAGGGAGGG
  TCTGGGTCAGGCCCTACCATTGAGGAGGTGGATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005346 unedited
  TCGATTTGTAAACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGGCTGCTGCGACAG
  TCCACTACCTTTTTCGAGAGTGACTCCCGTTGTCCCAAGGCTTCCCAGAGCGAACCTGTG
  CGGCTGCAGGCACCGGCGCGTCGAGTTTCCGGCGTCCGGAAGGACCGAGCTCTTCTCGCG
  GATCCAGTGTTCCGTTTCCAGCCCCTTTTCTCAGAGCCGAGCCGACAGAGAGCAGGGAAC
  CGGCATGGCCAAAGCCGCGGCGATCGGCATCGACCTGGGCACCACCTACTCCTGCGTGGG
  GGTGTTCCAACACGGCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACCACCCC
  CAGCTACGTGGCCTTCACGGACACCGAGCGGCTCATCGGGGATGCGGCCAAGAACCAGGT
  GGCGCTGAACCCGCAGAACACCGTGTTTGACGCGAAGCGGCTGATCGGCCGCAAGTTCGG
  CGACCCGGTGGTGCAGTCGGACATGAAGCACTGGCCTTTCCAGGTGATCAACGACGGAGA
  CAAGCCCAAGGTGCAGGTGAGCTACAAGGGGGACACCAAGGCATTCTACCCTGAGGAGAT
  CTCGTCCATGGTGCTGACCAAGATGAAGGAGATCGCCGAGGCGTACCTGTGCTACCCCGT
  GACCAACGCGGTGATCACCGTGCCGGCCTACTTCAACGACTCGCAGCGCCAGGCCCCCAA
  GGATGCGGGTGTGATCGCCGGGCTCAACGTGCTGCGGATCATCAACGAAGCCACGGCCGC
  CGCCATCGCCTACGGCCTGGACAGAACGGGCAAGGGGGACCGCAACGTGCTCATCTTTGA
  CCTGGGCGGAAGCACCTTTCACGTGTTCATTCTGACGATTGACAACGCATCTTCAAGGGG
  AGGCCACGGCCGGGGACCCCACCTGGTGGGGAGGACTTGAACACAGTCGTGAACCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005346 unedited
  ACCACTTTTTCTGCCCGCCCCCGCTTTCTANGTCGAGTTTTTCTTTTTTTTTTTTGAATT
  TTAAAGTTTATTTACTTTGAAGTAACCAACTTACCCATGGCCTGAGTTAAGTGTATTAAA
  AAGAAGAAATAGCCGTAAGATGGCAGTATAAATTCATCTCTGCATGTAGAAACCGGAAAA
  AAAGCAAGTTCAGTACTTCACCAAAAAATTTCAACATTGCAAACACAGGAAATTGAGAAC
  TGACAAACAGAAATACTACGAAATGCAAAGTCTTGAAGCTCCAAAACAAAAACAGCAATC
  TTGGAAAGGCCCCTAATCTACCTCCTCAATGGTGGGGCCTGACCCAGACCCTCCCTTGCG
  ACCCTGAGCCCCGAAGCCCCCAGGCCCGGGACCACCGGGACCCTGGTACAGTTCGCTGAT
  GATGGGCTTACACACCTGCTCCAGCTACTTCCTCTCGCGCTCAAACTCGACCTTCTCGGA
  CAAGGTCATCCCCGTCCCCCCACGATATGACCCCCTTGCCACTCCGTCAGCATCTTCTTC
  TTGACGGCCTTCCTGATCTTGCTCTTGAGTCCCTTCCCCTACCGGGCGCTCTTCCTGCTG
  AAGGCGTAGGACTCCACGGCGCTCTTCGTTTGAACCCTTCCCGCTGTATACCCATCCCCC
  TTTGCCCTGCACGACCCCTGTCCATGCCTCGACCCCCTCCTGTCAGGCCCCCTCCTCCCC
  GCACAGCCCTCCCCTGGCCCCTTCCGCCCTCCCGTCCGCCTCCTTCCCCCCGGATCCCCC
  CCCTCCCTTCTCCGGCTTTCTCCTCCCGTGTCCTACCCCGGGCTCCCGCGCCCCTCCCCC
  TCGTACGTCCCACCCCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005346
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005346.3, NP_005337.1
• RefSeq Size: 2551 bp
• RefSeq ORF: 1926 bp
• Locus ID: 3304
• UniProt ID: P08107
• Cytogenetics: 6p21.33
• Domains: HSP70
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome
• Gene Summary: This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]