IMPA1 (NM_005536) Human Untagged Clone

CAT#: SC116674

IMPA1 (untagged)-Human inositol(myo)-1(or 4)-monophosphatase 1 (IMPA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IMPA1
• Synonyms: IMP; IMPA; MRT59
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116674 sequence for NM_005536 edited (data generated by NextGen Sequencing)
  ATGGCTGATCCTTGGCAGGAATGCATGGATTATGCAGTAACTCTAGCAAGACAAGCTGGA
  GAGGTAGTTTGTGAAGCTATAAAAAATGAAATGAATGTTATGCTGAAAAGTTCTCCAGTT
  GATTTGGTAACTGCTACGGACCAAAAAGTTGAAAAAATGCTTATCTCTTCCATAAAGGAA
  AAGTATCCATCTCACAGTTTCATTGGTGAAGAATCTGTGGCAGCTGGGGAAAAAAGTATC
  TTAACCGACAACCCCACATGGATCATTGACCCTATTGATGGAACAACTAACTTTGTACAT
  AGATTTCCTTTTGTAGCTGTTTCAATTGGCTTTGCTGTAAATAAAAAGATAGAATTTGGA
  GTTGTGTACAGTTGTGTGGAAGGCAAGATGTACACTGCCAGAAAAGGAAAAGGTGCCTTT
  TGTAATGGTCAAAAACTACAAGTTTCACAACAAGAAGATATTACCAAATCTCTCTTGGTG
  ACTGAGTTGGGCTCTTCCAGAACACCAGAGACTGTGAGAATGGTTCTTTCTAATATGGAA
  AAGCTTTTTTGCATTCCTGTTCATGGGATCCGGAGTGTTGGAACAGCAGCTGTTAATATG
  TGCCTTGTGGCAACTGGCGGAGCAGATGCATATTATGAAATGGGAATTCACTGCTGGGAT
  GTTGCAGGAGCTGGCATTATTGTTACTGAAGCTGGTGGCGTGCTAATGGATGTTACAGGT
  GGACCATTTGATTTGATGTCACGAAGAGTAATTGCTGCAAATAATAGAATATTAGCAGAA
  AGGATAGCTAAAGAAATTCAGGTTATACCTTTGCAACGAGACGACGAAGATTAA
  
  Clone variation with respect to NM_005536.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005536 unedited
  TTCGGATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACGAGGCTCTACCTCCG
  GAAGAGACGAGTGCGGTAACACCGTTCACAGAGCTAGCCGGACGTCCTCCGACTCAAGAT
  ATTTGTCAAATATTTTCAGAAGATGGCTGATCCTTGGCAGGAATGCATGGATTATGCAGT
  AACTCTAGCAAGACAAGCTGGAGAGGTAGTTTGTGAAGCTATAAAAAATGAAATGAATGT
  TATGCTGAAAAGTTCTCCAGTTGATTTGGTAACTGCTACGGACCAAAAAGTTGAAAAAAT
  GCTTATCTCTTCCATAAAGGAAAAGTATCCATCTCACAGTTTCATTGGTGAAGAATCTGT
  GGCAGCTGGGGAAAAAAGTATCTTAACCGACAACCCCACATGGATCATTGACCCTATTGA
  TGGAACAACTAACTTTGTACATAGATTTCCTTTTGTAGCTGTTTCAATTGGCTTTGCTGT
  AAATAAAAAGATAGAATTTGGAGTTGTGTACAGTTGTGTGGAAGGCAAGATGTACACTGC
  CAGAAAAGGAAAAGGTGCCTTTTGTAATGGTCAAAAACTACAAGTTTCACAACAAGAAGA
  TATTACCAAATCTCTCTTGGTGACTGAGTTGNGCTCTTCCAGAACACCAGAGACTGTGAG
  AATGGNTCTTTCTAATATGGAAAAGCCTTTTTGCATTCCTGTTCATGGGATCCGGAGTGT
  TGGAACAGCAGCTGTTAATATGTGCCTTGTGGGCACTGGCGGAGCAGATGCATATTATGA
  AATGGGAATTTCACTGCTGGGATGTTGCCAGAGCTGCCATTATTGNTACTGAAAGCTGTG
  GCGTGCTAATGGATGTTACCAGNTGCACCATTGGATTTGATGCCCCGAAGAGTAATTGCT
  GCAATAATTAGATTATAGCCGAAAGAATACCTAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005536 unedited
  CCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATCAATTTAAAGTTTTA
  TTCATGATCGTCTTATTAAAATAAATTTAATTGCAAAAACAACCTGTCTTAGAATACAGT
  ATCAGATGTTTTAAGTACATGTGATCAACAGTACAAATAATTATAGCAGTCAAACCTTAG
  TATCACACATACTTAATATATTAGATATACACAATAATAAAATCACTCCCTACCTTGAAA
  ACTTTACAGAAGCATTTTTAATTTTACAACACAAAGCTCAAACGAACCTACAATAAGTCT
  AGTAGTCTGTTTACGTGCCAAGGGATAAGGCTGAACAATAAATTAACCCTTTAAAAATGT
  CTATGAACAAGTACAATTTTCTTTTTGAGTTCTGCAGAGCAATGACCACTAAGAAATATT
  TTTAAAGGCTGAACAGAATCCAGCGGCAATGAAGTTAATTAAATAAGAAGACTAAGAGAA
  AAATAAACAGTTCAATATTAACAATACAGTATATTTGCCAGCACAATCAAATCAGTATAA
  ACACCTTTAAAACATGAATACTGAACTTAGTAAAATGTTATATTGTATCTAATTATGTCA
  GATATCTGATGAGAGTGCTTTTTATTATAGAAATAGTTTCTTCTCTCTTACACAAGACTT
  TAAAGCAAATCCAAACAGACAATTTAACTGTTTTGGAAAATGTATTCACTGCAGAAATGC
  CCACAATAAGCCATGCTATCTGATGGTATGGTAATGTGAAATGGCATGGGTACGTGAATA
  AAAAAAATTAACTAGATAATTTTACTTCAAACCTGAGCAAAGTACAGAAGCTGAGTTCAT
  ACTTTAGTATCTATTGGACTAGGAATACTACATTAAATATTCATTCTACAAGGTTGTTTT
  TATTGCTAAATTGTATGGGCCCAGTGCTATATGACACTGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005536
• Insert Size: 2360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005536.2, NP_005527.1
• RefSeq Size: 2349 bp
• RefSeq ORF: 834 bp
• Locus ID: 3612
• UniProt ID: P29218
• Cytogenetics: 8q21.13
• Domains: inositol_P
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.