RGS4 (NM_005613) Human Untagged Clone

CAT#: SC116635

RGS4 (untagged)-Human regulator of G-protein signaling 4 (RGS4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RGS4
• Synonyms: RGP4; SCZD9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005613, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGCAAAGGGCTTGCAGGTCTGCCGGCTTCTTGCTTGAGGAGTGCAAAAGATATGAAACATCGGCTAG
  GTTTCCTGCTGCAAAAATCTGATTCCTGTGAACACAATTCTTCCCACAACAAGAAGGACAAAGTGGTTAT
  TTGCCAGAGAGTGAGCCAAGAGGAAGTCAAGAAATGGGCTGAATCACTGGAAAACCTGATTAGTCATGAA
  TGTGGGCTGGCAGCTTTCAAAGCTTTCTTGAAGTCTGAATATAGTGAGGAGAATATTGACTTCTGGATCA
  GCTGTGAAGAGTACAAGAAAATCAAATCACCATCTAAACTAAGTCCCAAGGCCAAAAAGATCTATAATGA
  ATTCATCTCAGTCCAGGCAACCAAAGAGGTGAACCTGGATTCTTGCACCAGGGAAGAGACAAGCCGGAAC
  ATGCTAGAGCCTACAATAACCTGCTTTGATGAGGCCCAGAAGAAGATTTTCAACCTGATGGAGAAGGATT
  CCTACCGCCGCTTCCTCAAGTCTCGATTCTATCTTGATTTGGTCAACCCGTCCAGCTGTGGGGCAGAAAA
  GCAGAAAGGAGCCAAGAGTTCAGCAGACTGTGCTTCCCTGGTCCCTCAGTGTGCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005613 unedited
  GTTANAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCGGAGAAGA
  GGCAAAGTACGCTCAAAGCCGAAGCCACAGCTCCTCCTGCCGCATTTCTTTCCTGCTTGC
  GAATTCCAAGCTGTTAAATAAGATGTGCAAAGGGCTTGCAGGTCTGCCGGCTTCTTGCTT
  GAGGAGTGCAAAAGATATGAAACATCGGCTAGGTTTCCTGCTGCAAAAATCTGATTCCTG
  TGAACACAATTCTTCCCACAACAAGAAGGACAAAGTGGTTATTTGCCAGAGAGTGAGCCA
  AGAGGAAGTCAAGAAATGGGCTGAATCACTGGAAAACCTGATTAGTCATGAATGTGGGCT
  GGCAGCTTTCAAAGCTTTCTTGAAGTCTGAATATAGTGAGGAGAATATTGACTTCTGGAT
  CAGCTGTGAAGAGTACAAGAAAATCAAATCACCATCTAAACTAAGTCCCAAGGCCAAAAA
  GATCTATAATGAATTCATCTCAGTCCAGGCAACCAAAGAGGTGAACCTGGATTCTTGCAC
  CAGGGAAGAGACAAGCCGGAACATGCTAGAGCCTACAATAACCTGCTTTGATGAGGCCCA
  GAAGAAGATTTTCAACCTGATGGAGAAGGATTCCTACCGCCGCTTCCTCAAGTCTCGATT
  CTATCTTGATTTGGTCAACCCGTCCAGCTGTGGGGCAGAAAAGCAGAAAGAGCCCAAGAT
  TCAGCAGACTGTGCTTCCCTGGTCCCTCAGTGTGCCTAATTCTCACCTGAGGCAGAAGGA
  TGAAATGCCCAGACTCTATGCTCTGGAAACCTGAGCCAATATTTGATCTGTATTAGGCTC
  CAGTGCTTTATCCACATTGTAGCCTATATTCATGCTGGCTGCCCTGGGGGGATTCACTCT
  ACGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005613 unedited
  TGGCAACTCTCCGGGGCCAGGNANAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT
  TTTTATTACATAAAATAATTTACTTCTCATTCAGCGTGACCTTCCAGACCTATTAGTAAG
  ACAGCAGTCTGTGAGTTCAGATACAATCTGAAAGTCATGACAATTATAATAGGGCAAGAC
  CAATAAAGTTACACATTTGAAGTCAACTAGAGACAAAATTGTGGAACTGTGTATAACAGA
  AAATGACACTGATGAGGAACTCCAGGGATATTAAGGGCAAGATTGCCAGAGAGACAAGAA
  GGGTACAATTAGAAAACCTGCATCTTTAATACAGTGAGATTTGTATGCATACACTCTGGT
  GTCTTCATTTTGCAGCCCATTATATTGGCTTAAAAGCCAGAAAGGTGCTCATGCATGTAA
  TTTATACTGGAGCAACGAATGCAGTGTATGTGGACGCGTGTGTGTGCACTTTGAAAGGCA
  GTAGTTTTAGAAGAATGAGAAGAAAATTACTTCCTGGGCATTCATTAATAATTCAGGTGA
  TATCCTGTACCTCCATTTATAAAACAATATAATAATAATAACACGTACAAAATAACATCA
  TAAATAACATAAATGGAGAGTTTTCAAGTATTTCAATGTGCCTTGAAAAGTTTCAAACAG
  TAATTCTCACAATTGGTTAGACAATCATTNTTTTTTCTTTTTCTTTCCTCTCATTTAGNC
  TCAGAGCCTAAAAAGAATGCCTNCAGTCTCTGGTACCCCCGATGTTGGAATGAANNNTAC
  AGATCTACCTTAAGAGAAACTATGTGGCTTTTCAATGTGAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005613
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005613.3, NP_005604.1
• RefSeq Size: 2769 bp
• RefSeq ORF: 618 bp
• Locus ID: 5999
• UniProt ID: P49798
• Cytogenetics: 1q23.3
• Domains: RGS
• Protein Families: Druggable Genome
• Gene Summary: Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has an alternate 5' sequence, which results in a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1.