p53 BP3 (TOPORS) (NM_005802) Human Untagged Clone

CAT#: SC116539

TOPORS (untagged)-Human topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (TOPORS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p53 BP3
• Synonyms: LUN; P53BP3; RP31; TP53BPL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005802, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGTCGCAGCCGCCGCTGGGGTCTCCGCTGTCTCGCGAGGAGGGTGAAGCGCCCCCGCCTGCTCCCG
  CTTCGGAGGGTAGGCGGAGAAGTCGCCGGGTACGCCTTCGCGGATCCTGCCGACACCGACCTAGCTTTCT
  GGGCTGCCGGGAGCTCGCGGCGAGCGCCCCAGCCAGGCCTGCGCCGGCATCCTCCGAGATAATGGCATCA
  GCTGCTAAGGAATTTAAAATGGACAACTTTTCACCTAAAGCTGGCACTAGCAAATTGCAACAGACAGTAC
  CAGCTGATGCATCTCCTGATTCTAAGTGTCCTATATGCTTGGATAGATTTGATAATGTGTCTTACTTAGA
  TCGCTGCTTACATAAGTTCTGTTTTCGCTGTGTACAGGAGTGGTCAAAAAACAAAGCTGAATGCCCACTA
  TGTAAACAGCCCTTTGATTCTATTTTCCATTCTGTGAGGGCAGAAGATGACTTCAAGGAGTATGTCCTAA
  GGCCTTCGTATAATGGTTCTTTTGTCACCCCTGATCGACGATTTCGCTACCGTACAACTCTGACAAGGGA
  ACGAAATGCTTCTGTGTATTCACCTAGTGGTCCTGTGAACAGAAGAACAACAACTCCACCGGATAGTGGA
  GTACTGTTTGAAGGGTTAGGCATTTCAACAAGACCTAGAGATGTTGAAATTCCTCAGTTTATGAGACAGA
  TTGCAGTAAGGAGGCCAACTACGGCAGATGAAAGATCTTTGCGGAAAATTCAAGAACAAGATATTATTAA
  TTTTAGACGAACTCTTTATCGTGCTGGTGCTCGAGTTAGAAATATTGAAGATGGTGGCCGCTACAGGGAT
  ATTTCAGCTGAATTTTTCCGTAGAAATCCAGCTTGCCTTCACAGATTAGTCCCCTGGTTAAAACGTGAAC
  TTACAGTTCTTTTTGGAGCTCATGGATCTTTAGTGAATATTGTCCAGCATATTATCATGAGTAATGTTAC
  TCGCTATGACTTGGAGAGTCAGGCATTTGTGTCTGATTTAAGACCATTTTTACTTAATCGAACTGAGCAT
  TTTATACATGAATTTATCAGTTTTGCCCGATCTCCTTTTAACATGGCAGCCTTTGACCAGCATGCCAATT
  ATGATTGCCCTGCTCCTTCATACGAAGAAGGCAGCCATTCTGATTCTTCAGTCATAACAATATCTCCAGA
  TGAGGCTGAGACCCAAGAGCTGGATATTAATGTAGCCACTGTTAGTCAGGCACCATGGGATGATGAAACT
  CCAGGACCATCTTACTCAAGCTCAGAGCAGGTACACGTTACTATGTCTTCTCTTTTAAATACTTCTGACA
  GTTCAGATGAAGAACTTGTCACAGGAGGAGCCACGTCTCAGATACAAGGAGTACAAACCAATGACGACCT
  AAATAATGACAGTGATGATTCTTCAGATAATTGTGTCATTGTTGGGTTTGTTAAACCACTAGCTGAGAGG
  ACCCCAGAACTTGTTGAACTGTCCTCTGATTCTGAGGACTTAGGTTCTTATGAGAAAATGGAGACAGTGA
  AGACACAAGAACAGGAGCAATCTTACAGTTCTGGTGATAGCGATGTTAGTAGATGCTCATCTCCACACTC
  TGTCCTTGGAAAGGATGAACAAATAAATAAAGGTCATTGTGATTCTAGTACAAGAATCAAATCAAAGAAG
  GAAGAGAAACGATCTACATCATTGTCATCTCCCAGAAACCTGAACTCATCTGTAAGAGGAGACAGAGTAT
  ATTCTCCATATAACCATAGACACAGAAAGAGGGGAAGATCAAGAAGTTCAGATTCACGTTCTCAGAGTAG
  AAGTGGGCATGATCAGAAGAATCATAGAAAGCATCATGGGAAGAAAAGAATGAAAAGTAAACGATCCAGA
  AGCAGGGAAAGTAGCAGACCTAGAGGGAGAAGAGACAAAAAGAGATCAAGAACTAGAGATAGCAGTTGGT
  CCAGAAGAAGCCAAACTCTGTCTCTAAGTAGTGAAAGCACAAGCAGATCAAGGTCTCGTAGCAGTGATCA
  TGGTAAAAGAAGATCACGGAGCAGAAATAGAGATCGTTATTATTTAAGAAATAATTATGGAAGCAGATAC
  AAATGGGAGTATACTTATTACAGTAGAAACAAGGACAGGGATGGGTACGAATCATCTTACAGGAGGAGGA
  CTCTGTCCAGAGCTCATTATTCTAGACAGTCTTCAAGTCCAGAATTTAGAGTTCAGTCCTTTTCTGAAAG
  AACAAATGCTAGGAAAAAAAATAATCACAGTGAGAGGAAGTATTACTACTATGAAAGGCACAGATCAAGG
  AGCCTGTCTAGTAACAGATCAAGGACTGCATCTACCGGGACTGACCGGGTGAGAAATGAAAAGCCTGGAG
  GGAAACGAAAATACAAAACACGGCATTTGGAGGGTACTAACGAAGTGGCTCAGCCATCTCGTGAATTTGC
  TTCTAAAGCAAAGGACAGTCATTACCAAAAATCTTCATCAAAATTGGATGGAAACTACAAAAATGAGAGT
  GATACCTTTTCAGACAGCCGATCATCAGACAGAGAGACAAAACACAAAAGGAGAAAAAGGAAGACCCGGA
  GCCTAAGTGTAGAGATAGTTTATGAAGGAAAAGCTACTGATACAACTAAACACCATAAAAAGAAAAAGAA
  GAAACATAAGAAGAAGCATAAGAAACACCATGGAGATAATGCTTCACGTTCCCCAGTTGTAATTACCATT
  GACAGTGACAGTGATAAGGATTCTGAAGTAAAGGAGGATACAGAATGTGACAATAGTGGTCCTCAAGACC
  CTCTACAAAATGAGTTTTTGGCTCCTTCCTTGGAACCATTTGAAACTAAAGATGTAGTTACAATAGAAGC
  TGAATTTGGTGTGCTGGACAAGGAATGTGATATTGCCACACTTAGTAACAACTTGAATAATGCCAACAAA
  ACTGTAGATAATATTCCACCTCTGGCAGCTTCAGTTGAACAAACTCTCGATGTAAGAGAAGAGAGCACCT
  TTGTTTCTGATTTGGAGAACCAGCCCAGTAACATTGTGTCTCTTCAAACTGAGCCATCAAGGCAATTGCC
  ATCGCCACGGACATCATTAATGTCAGTATGTCTTGGTAGAGACTGTGATATGTCTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005802 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTCTATTTTCCATTCTGTGA
  GGGCAGAAGATGACTTCAAGGAGTATGTCCTAAGGCCTTCGTATAATGGTTCTTTTGTCA
  CCCCTGATCGACGATTTCGCTACCGTACAACTCTGACAAGGGAACGAAATGCTTCTGTGT
  ATTCACCTACTGGTCCTGTGAACAGAAGAACAACAACTCCACCGGATAGTGGAGTACTGT
  TTGAAGGGTTAGGCATTTCAACAAGACCTAGAGATGTTGAAATTCCTCAGTTTATGAGAC
  AGATTGCAGTAAGGACGCCAACTACGGCAGATGAAAGATCTTTGCGGAAAATTCAAGAAC
  AAGATATTATTAATTTTAGACGAACTCTTTATCGTGCTGGTGCTCGAGTTAGAAATATTG
  AAGATGGTGGCCGCTACAGGGATATTTCAGCTGAATTTTTCCGTAGAAATCCAGCTTGCC
  TTCACAGATTAGTCCCCTGGTTAAAACGTGAACTTACAGTTCTTTTTGGAGCTCATGGAT
  CTTTAGTGAATATTGTCCAGCATATTATCATGAGTAATGTAACTTGCTATGACTTGAAAA
  GTCAAGCATTTGGGTCTTGATTTAAAACCCATTTTTACTTATCCGACTGACCCATTTTAT
  CCATGAATTTAATTAGTTTTGCCCCGATCTCCTTTTTAACATGGCAGCTCTTGACCCCCA
  TGCCAATTATGATTGCCCTGCTCCTTCTACCAAAAAGGAGCCTTTTTGTTCTTTAATCTT
  ACAATATTCTCCGAATGAGGCGGACACCCAAAACTTGTTTTAAAGGCACCACTGTTTACT
  CCGGCCCTTGGGAAGATGGATATTCAGACACCTTTTCTTCAACTTAAACCAGGCCCCCGT
  CCCTATCGCCTTTTTTTAAACCTTCGAAGGAAAAAAAAAAACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005802 unedited
  CACGTGTACGAGCCGCCGTCGAGATCGAGTTAATTTCTTTTTTGTTTCTTGTTTTAAAGT
  GTTACTGGTTTATTTTTTTTCTTTTGTCGAATACACATTTGTTTTACTGGCAGTCCAGAT
  AAACAAGTTTATAAAGGAAATAGTAAATTTTTATTTTGGCAGAAGATAAACTGTGCAGAT
  TAAAATGCCTCAATTTAACCTGAAGAAAGGTTCTGATCATATAAATTTTTAAGAAGAAAA
  ATAAAGACTGTCCAAAGGGATTCTCCTAGCTTATAACACATTTCCAGAATAACTCTCAAA
  AAAAAAAAATTCAAAAATACTCATTGACTACCTTAAAAATATGTCAATACCGTTTGGCTT
  TATGAAATAACTTCTAAAATTTATATAATTTTTCTTATTTAAAAAGGACCCCAAGTTTTA
  AATAAGCTGCTACCAGTCTCATTTTCTTCACTTAAAAGTGCATATCTTTGAGGGTGGGAC
  ATCCCTTTTGAACAAATAATGATTTTTTACAATTACCCCCCACACAAAAATCTTTTATAA
  TTTTTGCAGGTGGAAGAGAGTTTTCCCCAAATGTCATCTTTTAATCGACTGCGGTGGACG
  ACATTCTTCCTTTTCCCTTTTTTTACCTCCTTCTTCTCCCTGAAATGCCTTCGGCCGCTT
  TCCGACTCCTCCCAGTCTTTCCCCTCTCTTCCTGCCCTTTACGGAGCCCCCGGGCCCTTG
  GCCTTTGGCCTCTGTTGCCCACTTTTGGNGATCCCCCCTCGTTCCGGGCCTGGTTTTCTT
  CTCCTCCCACGCGGGCGGTCTTTTTTTTCTTTTCCTTGGGGTTCTGTCCTCCTTGTGGCC
  TCGCCTTGGGGGGGATTTTTTCCTTCCCTCTTGTCGGCCCTTTCTTTGTGTCTGCTCCCC
  CGCGGGTGGTCTCCGTCCGTCGCCTGCGCGCCGCACTCGTTTCCCGCTCCTCTTGTCCCT
  CCCCTGTCTAACTGATAATAACACACAAAACAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005802
• Insert Size: 3370 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005802.1, NP_005793.1
• RefSeq Size: 4183 bp
• RefSeq ORF: 2448 bp
• Locus ID: 10210
• UniProt ID: Q9NS56
• Cytogenetics: 9p21.1
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a nuclear protein which is serine and arginine rich, and contains a RING-type zinc finger domain. It is highly expressed in the testis, and functions as an ubiquitin-protein E3 ligase. Mutations in this gene are associated with retinitis pigmentosa type 31. Alternatively spliced transcript variants, encoding different isoforms, have been observed for this locus. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).