AP3S2 (NM_005829) Human Untagged Clone

CAT#: SC116481

AP3S2 (untagged)-Human adaptor-related protein complex 3, sigma 2 subunit (AP3S2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP3S2
• Synonyms: AP3S3; sigma3b
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005829 edited
  ATGATTCAGGCGATTCTGGTTTTCAACAACCATGGGAAGCCACGGCTAGTCCGCTTCTAC
  CAGCGTTTCCCAGAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTAGTCCTC
  AAGCGGGATGACAACATCTGTAACTTCTTGGAGGGTGGAAGTTTGATTGGTGGCTCTGAC
  TACAAACTGATCTACCGGCACTATGCTACCCTCTACTTTGTATTTTGTGTGGATTCCTCA
  GAGAGTGAACTTGGAATCTTGGACCTCATCCAGGTTTTTGTGGAAACTCTGGATAAGTGT
  TTCGAAAATGTGTGTGAATTGGATTTGATCTTCCATATGGATAAGGTGCACTACATCCTC
  CAGGAGGTGGTGATGGGTGGGATGGTGTTGGAAACAAACATGAATGAAATCGTGGCTCAG
  ATTGAGGCTCAAAACAGGCTGGAGAAATCCGAGGGTGGCCTTTCAGCAGCCCCTGCGCGG
  GCTGTGTCTGCTGTGAAAAACATCAACCTGCCAGAGATTCCTCGGAACATCAACATTGGC
  GATCTCAACATCAAAGTTCCCAACCTGTCCCAGTTTGTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005829 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGATGATTCAGGC
  GATTCTGGTTTTCAACAACCATGGGNAAGCCACGGCTAGTCCGCTTCTACCAGCGTTTCC
  CAGAAGAAATTCAACAGCAGATTGTTCGAGAGACTTTCCATCTAGTCCTCAAGCGGGATG
  ACAACATCTGTAACTTCTTGGAGGGTGGAAGTTTGATTGGTGGCTCTGACTACAAACTGA
  TCTACCGGCACTATGCTACCCTCTACTTTGTATTTTGTGTGGATTCCTCAGAGAGTGAAC
  TTGGAATCTTGGACCTCATCCAGGTTTTTGTGGAAACTCTGGATAAGTGTTTCGAAAATG
  TGTGTGAATTGGATTTGATCTTCCATATGGATAAGGTGCACTACATCCTCCAGGAGGTGG
  TGATGGGTGGGATGGTGTTGGAAACAAACATGAATGAAATCGTGGCTCAGATTGAGGCTC
  AAAACAGGCTGGAGAAATCCGAGGGTGGCCTTTCAGCAGCCCCTGCGCGGGCTGTGTCTG
  CTGTGAAAAACATCAACCTGCCAGAGATTCCTCGGAACATCAACATTGGCGATCTCAACA
  TCAAAGTTCCCAACCTGTCCCAGTTTGTCTGANGATCAAGTATTGGCCTGAAATAGAGTC
  CTTAAGACAAGCAAAGACAAGCAAGGCAAGCACGTCTGGAAACAGAACCCATTTTGAGCC
  TTAGAAGAGTCAAGCCTCAGGACCTGGAAACTTTGTGTCTGGGGAAGACTGTTTGGCATG
  GATAAGGGAAGGGATTCCTATTGACACTGCTCGGGTGCACCCAGTTCTCACATGTGCAGT
  CATGCCGTTCTCTGATGCATACGGCCCTTGAGATGGAGAGGGCCCCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005829 unedited
  CGCGCGGCCCCAACACAAACACATTATCTNNCNNNNGCNNTTACTGTGNNCNCACGGNCC
  GCTATTTTANGANCGAGTTTTTTTTTTTTTTTTTTGCTCTCATGAGGAGATGTATTTTAA
  ACTTGGGAAGAGTCATAATTCTGGGATGTTTCACATGTTGTCAGCTTTAACCTTCTACAG
  ACACAGGCCCTCTCCTCTGTGAGGAGGGACCTCTGGCATGTGTGGGTGTGTGGTGGGTCC
  CTCTCCCTATTAGCAGAAATGTGTTGGGCATGAGCCAGGTTTATGATTTGGATTGTGTCC
  TGCACATAACACCTGTGAGAATACAACTGGGACTAGGACAATGCGGGAAGCATATTCTTC
  ATGAGGCGGTAACCAAAAGGCTTGGCTATACCAAAGGATTCTGGTGGCCGGGCACGGTGG
  CTCACACCTGTAATGCCAGCACTTTGGGAGGCCAAGGCGGGTAGATCACTTGAGGTCCAG
  GAGTTCGAGCCCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAACATACAACAAT
  TAGCTGGGCATGGTGGTGGGTGCCTGTAATCCCAGCTACTGNGGAGGCTGAGACAGGAGA
  AATGCTTGAACCCGGGAAGCAGAGGTTGCAGTGAGCCGAGATTGCACCACAGCACTCCAG
  CCTGNGTGACAGAGGCAGACTCCACCTCAAAAAAAAAAACCCCAAAAAAACACAAAAGGG
  ATTCTGGGTTGTAACAATTCTCANTAAGAGCCAGCGGGTGGACTCTTGGTTCTCCTAAAA
  ATTCTGCCCCTTATTTGCCACTACCCACACCCCCCAGATCTGAAAGGGCCAGCCCTTTTT
  TTACCTATGGAACCCTCACCCAGCAGTTAAAGGCCACCAGGACCTCTAAAAGTCTGTGGT
  GCCTAAGATGAAGACTCTTCTCAAAAAAAGGGGCTCACCCCTAATAAGGAGGTTTTGGAA
  ACAAGGTGGCCTGCTAAATGCAACCCTTACCCTTGGGAGCTTCCTTGGAAAAGGAAAGGG
  CCAAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005829
• Insert Size: 1830 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005829.3, NP_005820.1
• RefSeq Size: 2578 bp
• RefSeq ORF: 582 bp
• Locus ID: 10239
• UniProt ID: P59780
• Cytogenetics: 15q26.1
• Domains: Clat_adaptor_s
• Protein Pathways: Lysosome
• Gene Summary: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shortest transcript but encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.