SMAD1 (NM_005900) Human Untagged Clone

CAT#: SC116433

SMAD1 (untagged)-Human SMAD family member 1 (SMAD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMAD1
• Synonyms: BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005900 edited
  GAATTCGGCACGAGGGGCGGGCGGGCGGAGAAAGGAGAGGCCGAGCGGCTCAACCCGGGC
  CGAGGCTCGGGGAGCGGAGAGTGGCGCAGCGCCCGGCCGTCCGGACCCGGGCCGCGAGAC
  CCCGCTCGCCCGGCCACTCGTGCTCCCGCACGGACGGGCGCGCCGCCAACCCGGTGCTGA
  CTGGGTTACTTTTTTAAACACTAGGAATGGTAATTTCTACTCTTCTGGACTTCAAACTAA
  GAAGTTAAAGAGACTTCTCTGTAAATAAACAAATCTCTTCTGCTGTCCTTTTGCATTTGG
  AGACAGCTTTATTTCACCATATCCAAGGAGTATAACTAGTGCTGTCATTATGAATGTGAC
  AAGTTTATTTTCCTTTACAAGTCCAGCTGTGAAGAGACTTCTTGGGTGGAAACAGGGCGA
  TGAAGAAGAAAAATGGGCAGAGAAAGCTGTTGATGCTTTGGTGAAAAAACTGAAGAAAAA
  GAAAGGTGCCATGGAGGAACTGGAAAAGGCCTTGAGCTGCCCAGGGCAACCGAGTAACTG
  TGTCACCATTCCCCGCTCTCTGGATGGCAGGCTGCAAGTCTCCCACCGGAAGGGACTGCC
  TCATGTCATTTACTGCCGTGTGTGGCGCTGGCCCGATCTTCAGAGCCACCATGAACTAAA
  ACCACTGGAATGCTGTGAGTTTCCTTTTGGTTCCAAGCAGAAGGAGGTCTGCATCAATCC
  CTACCACTATAAGAGAGTAGAAAGCCCTGTACTTCCTCCTGTGCTGGTTCCAAGACACAG
  CGAATATAATCCTCAGCACAGCCTCTTAGCTCAGTTCCGTAACTTAGGACAAAATGAGCC
  TCACATGCCACTCAACGCCACTTTTCCAGATTCTTTCCAGCAACCCAACAGCCACCCGTT
  TCCTCACTCTCCCAATAGCAGTTACCCAAACTCTCCTGGGAGCAGCAGCAGCACCTACCC
  TCACTCTCCCACCAGCTCAGACCCAGGAAGCCCTTTCCAGATGCCAGCTGATACGCCCCC
  ACCTGCTTACCTGCCTCCTGAAGACCCCATGACCCAGGATGGCTCTCAGCCGATGGACAC
  AAACATGATGGCGCCTCCCCTGCCCTCAGAAATCAACAGAGGAGATGTTCAGGCGGTTGC
  TTATGAGGAACCAAAACACTGGTGCTCTATTGTCTACTATGAGCTCAACAATCGTGTGGG
  TGAAGCGTTCCATGCCTCCTCCACAAGTGTGTTGGTGGATGGTTTCACTGATCCTTCCAA
  CAATAAGAACCGTTTCTGCCTTGGGCTGCTCTCCAATGTTAACCGGAATTCCACTATTGA
  AAACACCAGGCGGCATATTGGAAAAGGAGTTCATCTTTATTATGTTGGAGGGGAGGTGTA
  TGCCGAATGCCTTAGTGACAGTAGCATCTTTGTGCAAAGTCGGAACTGCAACTACCATCA
  TGGATTTCATCCTACTACTGTTTGCAAGATCCCTAGTGGGTGTAGTCTGAAAATTTTTAA
  CAACCAAGAATTTGCTCAGTTATTGGCACAGTCTGTGAACCATGGATTTGAGACAGTCTA
  TGAGCTTACAAAAATGTGTACTATACGTATGAGCTTTGTGAAGGGCTGGGGAGCAGAATA
  CCACCGCCAGGATGTTACTAGCACCCCCTGCTGGATTGAGATACATCTGCACGGCCCCCT
  CCAGTGGCTGGATAAAGTTCTTACTCAAATGGGTTCACCTCATAATCCTATTTCATCTGT
  ATCTTAAATGGCCCCAGGCATCTGCCTCTGGAAAACTATTGAGCCTTGCATGTACTTGAA
  GGATGGATGAGTCAGATACGATTGAGAACTGACAAAGGAGCCTTGATAATACTTGACCTC
  TGTGACCAACTGTTGGATTCAGAAATTTAAACAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005900 unedited
  GTGTTCGAATATTTGTATACGACTCACTATAGGGCGGCCGCGNAATCGGCACGAGGGGCG
  GGCGGGCGGAGAAAGGAGAGGCCGAGCGGCTCAACCCGGGCCGAGGCTCGGGGAGCGGAG
  AGTGGCGCAGCGCCCGGCCGTCCGGACCCGGGCCGCGAGACCCCGCTCGCCCGGCCACTC
  GTGCTCCCGCACGGACGGGCGCGCCGCCAACCCGGTGCTGACTGGGTTACTTTTTTAAAC
  ACTAGGAATGGTAATTTCTACTCTTCTGGACTTCAAACTAAGAAGTTAAAGAGACTTCTC
  TGTAAATAAACAAATCTCTTCTGCTGTCCTTTTGCATTTGGAGACAGCTTTATTTCACCA
  TATCCAAGGAGTATAACTAGTGCTGTCATTATGAATGTGACAAGTTTATTTTCCTTTACA
  AGTCCAGCTGTGAAGAGACTTCTTGGGTGGAAACAGGGCGATGAAGAAGAAAAATGGGCA
  GAGAAAGCTGTTGATGCTTTGGTGAAAAAACTGAAGAAAAAGAAAGGTGCCATGGAGGAA
  CTGGAAAAGGCCTTGAGCTGCCCAGGGCAACCGAGTAACTGTGTCACCATTCCCCGCTCT
  CTGGATGGCAGGCTGCAAGTCTCCCACCGGAAGGGACTGCCTCATGTCATTTACTGCCGT
  GTGTGGCGCTGGCCCGATCTTCAGAGCCACCATGAACTAAAACCACTGGGATGCTGTGAG
  TTTCCTTTTGGTTCCAAGCAGAAAGAGGTCTGCATCAATCCCTACCACTATAAGAGAGTA
  GAAAGCCCTGTACTTCCTCCTGTGCTGGGTCCAAGACACAGCGAATATAATCCTCAGCAC
  AGCCTCTTAGCTCAGGTTCCGTAACTTAGGACAAAAAGAGCCTCACATGGCACTCCACGC
  CACTTTTCCAGATTTTTTCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005900 unedited
  GCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTAAATTTCTGAATCCA
  ACAGTTGGTCACAGAGGTCAAGTATTATCAAGGCTCCTTTGTCAGTTCTCAATCGTATCT
  GACTCATCCATCCTTCAAGTACATGCAAGGCTCAATAGTTTTCCAGAGGCAGATGCCTGG
  GGCCATTTAAGATACAGATGAAATAGGATTATGAGGTGAACCCATTTGAGTAAGAACTTT
  ATCCAGCCACTGGAGGGGGCCGTGCAGATGTATCTCAATCCAGCAGGGGGTGCTAGTAAC
  ATCCTGGCGGTGGTATTCTGCTCCCCAGCCCTTCACAAAGCTCATACGTATAGTACACAT
  TTTTGTAAGCTCATAGACTGTCTCAAATCCATGGTTCACAGACTGTGCCAATAACTGAGC
  AAATTCTTGGTTGTTAAAAATTTTCAGACTACACCCACTAGGGATCTTGCAAACAGTAGT
  AGGATGAAATCCATGATGGTAGTTGCAGTTCCGACTTTGCACAAAGATGCTACTGTCACT
  AAGGCATTCGGCATACACCTCCCCTCCAACATAATAAAGATGAACTCCTTTTCCAATATG
  CCGNCTGGTGTTTTCATAGTGGAATTCCGGTTAACATTGGAGAGCAGCCCAAGGCAGAAA
  CGGTTCCTATTGTTGGGAAGGATCAGTGAAACCATTCACCCACACACTTGTGGAGGAAGC
  ATGAAACGCTTCCCCCCACGATTGTTGACCTCATATAGACATTAAACACCAGTGTTTTGG
  TTCCTTATTAACAACCGGCTGAACATTTCCTCTGTTGATTTCTGAGGCCAAGGGAGGGCC
  CATCATGGTTTGGGTCCCTCGGCTGAGACCCTACCTGGGTCATGGGGCTCTTTAGAAGGC
  AGGAACCAGGGTGGGGGCGTTTCACCCTGCCTTCTGGAAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005900
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005900.2, NP_005891.1
• RefSeq Size: 3056 bp
• RefSeq ORF: 1398 bp
• Locus ID: 4086
• UniProt ID: Q15797
• Cytogenetics: 4q31.21
• Domains: DWB, DWA, MH1
• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. All eight variants encode the same protein.