PKC delta (PRKCD) (NM_212539) Human Untagged Clone

CAT#: SC116249

PRKCD (untagged)-Human protein kinase C, delta (PRKCD), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKC delta
• Synonyms: ALPS3; CVID9; MAY1; nPKC-delta; PKCD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_212539 edited
  ATGGCGCCGTTCCTGCGCATCGCCTTCAACTCCTATGAGCTGGGCTCCCTGCAGGCCGAG
  GACGAGGCGAACCAGCCCTTCTGTGCCGTGAAGATGAAGGAGGCGCTCAGCACAGAGCGT
  GGGAAAACACTGGTGCAGAAGAAGCCGACCATGTATCCTGAGTGGAAGTCGACGTTCGAT
  GCCCACATCTATGAGGGGCGCGTCATCCAGATTGTGCTAATGCGGGCAGCAGAGGAGCCA
  GTGTCTGAGGTGACCGTGGGTGTGTCGGTGCTGGCCGAGCGCTGCAAGAAGAACAATGGC
  AAGGCTGAGTTCTGGCTGGACCTGCAGCCTCAGGCCAAGGTGTTGATGTCTGTTCAGTAT
  TTCCTGGAGGACGTGGATTGCAAACAGTCTATGCGCAGTGAGGACGAGGCCAAGTTCCCA
  ACGATGAACCGCCGCGGAGCCATCAAACAGGCCAAAATCCACTACATCAAGAACCATGAG
  TTTATCGCCACCTTCTTTGGGCAACCCACCTTCTGTTCTGTGTGCAAAGACTTTGTCTGG
  GGCCTCAACAAGCAAGGCTACAAATGCAGGCAATGTAACGCTGCCATCCACAAGAAATGC
  ATCGACAAGATCATCGGCAGATGCACTGGCACCGCGGCCAACAGCCGGGACACTATATTC
  CAGAAAGAACGCTTCAACATCGACATGCCGCACCGCTTCAAGGTTCACAACTACATGAGC
  CCCACCTTCTGTGACCACTGCGGCAGCCTGCTCTGGGGACTGGTGAAGCAGGGATTAAAG
  TGTGAAGACTGCGGCATGAATGTGCACCATAAATGCCGGGAGAAGGTGGCCAACCTCTGC
  GGCATCAACCAGAAGCTTTTGGCTGAGGCCTTGAACCAAGTCACCCAGAGAGCCTCCCGG
  AGATCAGACTCAGCCTCCTCAGAGCCTGTTGGGATATATCAGGGTTTCGAGAAGAAGACC
  GGAGTTGCTGGGGAGGACATGCAAGACAACAGTGGGACCTACGGCAAGATCTGGGAGGGC
  AGCAGCAAGTGCAACATCAACAACTTCATCTTCCACAAGGTCCTGGGCAAAGGCAGCTTC
  GGGAAGGTGCTGCTTGGAGAGCTGAAGGGCAGAGGAGAGTACTTTGCCATCAAGGCCCTC
  AAGAAGGATGTGGTCCTGATCGACGACGACGTGGAGTGCACCATGGTTGAGAAGCGGGTG
  CTGACACTTGCCGCAGAGAATCCCTTTCTCACCCACCTCATCTGCACCTTCCAGACCAAG
  GACCACCTGTTCTTTGTGATGGAGTTCCTCAACGGGGGGGACCTGATGTACCACATCCAG
  GACAAAGGCCGCTTTGAACTCTACCGTGCCACGTTTTATGCCGCTGAGATAATGTGTGGA
  CTGCAGTTTCTACACAGCAAGGGCATCATTTACAGGGACCTCAAACTGGACAATGTGCTG
  TTGGACCGGGATGGCCACATCAAGATTGCCGACTTTGGGATGTGCAAAGAGAACATATTC
  GGGGAGAGCCGGGCCAGCACCTTCTGCGGCACCCCTGACTATATCGCCCCTGAGATCCTA
  CAGGGCCTGAAGTACACATTCTCTGTGGACTGGTGGTCTTTCGGGGTCCTTCTGTACGAG
  ATGCTCATTGGCCAGTCCCCCTTCCATGGTGATGATGAGGATGAACTCTTCGAGTCCATC
  CGTGTGGACACGCCACATTATCCCCGCTGGATCACCAAGGAGTCCAAGGACATCCTGGAG
  AAGCTCTTTGAAAGGGAACCAACCAAGAGGCTGGGAGTGACGGGAAACATCAAAATCCAC
  CCCTTCTTCAAGACCATAAACTGGACTCTGCTGGAAAAGCGGAGGTTGGAGCCACCCTTC
  AGGCCCAAAGTGAAGTCACCCAGAGACTACAGTAACTTTGACCAGGAGTTCCTGAACGAG
  AAGGCGCGCCTCTCCTACAGCGACAAGAACCTCATCGACTCCATGGACCAGTCTGCATTC
  GCTGGCTTCTCCTTTGTGAACCCCAAATTCGAGCACCTCCTGGAAGATTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_212539 unedited
  CACCCATTCCGCACGAGGCCGCGGCGCCGGAGCCCGAGGCGGCTGTAGCCCACATCTCCC
  GATCGACCCCCGGCGCCCGCCCGCCGCGCGGAGGCCCGGGCCACACCTCACTGGCCGCTT
  GGCCCATCCCAGTCAGCGCCGCGCCGAACCCCGTCCGCGCGCGCCGGGGAGCGGCTTTCT
  CTTCGCTGCCGCCGCGACCCTTGGCGCCTGCCCCTGCAACGGGAGCCCCACTGCAGGCCC
  CACCATGGCGCCGTTCCTGCGCATCGCCTTCAACTCCTATGAGCTGGGCTCCCTGCAGGC
  CGAGGACGAGGCGAACCAGCCCTTCTGTGCCGTGAAGATGAAGGAGGCGCTCAGCACAGA
  GCGTGGGAAAACACTGGTGCAGAAGAAGCCGACCATGTATCCTGAGTGGAAGTCGACGTT
  CGATGCCCACATCTATGATGGGCGCGTCATCCAGATTGTGCTAATGCGGGCAGCAGAGGA
  GCCAGTGTCTGAGGTGACCGTGGGTGTGTCGGTGCTGGCCGAGCGCTGCAAGAAGAACAA
  TGGCAAGGCTGAGTTCTGGCTGGACCTGCAGCCTCAGGCCAAGGTGTTGATGTCTGTTCA
  GTATTTCCTGGAGGACGTGGATTGCAAACAGTCTATGCGCAGTGAGGACGATGCCAAGTT
  CCCAACGATGAACCGCCGCGGAGCCATCAAACAGGCCAAAATCCACTACATCAAGAACCA
  TGAGTTTATCGCCACCTTCTTTGGGCAACCCACCTTCTGTTCTGTGTGCAAAGACTTTGT
  CTGGGGCCCTCAACAAGCAAGGCTACAAATGCAAGCNATGTNACGCTGCCATCACAAGAA
  ATGCATCGACCAGATCATCGGCAGATGCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_212539 unedited
  CAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTGTCATATATATATTTTTTTGAAAAAGA
  CAACATATCACCAGTCCCCCATGGTTTTTCTCTACTGAGCAATATATACAGCCTATTATA
  TATATATATATGTCTATGTATATAATATATATTTTCAATAGATAACTACATTCAAGTAAT
  GAAACACAGGATTTACAATTTCCCTCCGAAGGCAGAGGAAAAGCAGATTCACACACAGTT
  CACAGAGGAAGTACCATGAGAGCCCGGCCAGACGGCAGCCAAGGACGCTCTGGGGGCAGG
  GGCGGGGCCAGCAGCAGAAGTCACCACAGTCCCACTGGTGCTTATCGTGGGGAGCGGGCA
  TGTGTGGGTGGAGGGCACGGCTAGCCTGATCTGTCCAGGAACCTCAATCTTCCAGGAGGT
  GCTCGAATTTGGGGTTCACAAAGGAGAACCCAGCGAATGCACACTGGTCCATGGAGCCGA
  TGAGGTTCTTGTCGCTGTAAGAGAGGCGCGCCTTCTTCCTCACGAACTCCCGGTCAAAGT
  TACCGTAGCCTCTGGGGGACTTCACTTTGGGCCCTGAAGGCGGCTCCAACCTCCCCTCTT
  TCATCATATTCCACCCCATCGCCCCCCAGACGGCGCGGACTCTGATGTTTCCCGCACTCC
  CATTCTCTTCGGCGGGCCCCCCTTTACACAGCCTTCCTCATAACTTTCTTTGCACCCCCC
  GCCGACCCACTCCGGACAATGTGGTTGCGTTCCCCCTCCTCGCCCCACACTAGTATCTCC
  ATATATTTATCCCAGAATCGGGAACCGCCCCCCCCCTCTTTCTTTTCCTCCCCCTGTTTT
  ATCCCGTTTCTCTTATTCCCCCCCGCCCCCCCTGCTTCCCCCGTTCTATTTTTTCTCTCC
  ATCCGCCCCCTCCCGCTTTCCTCTTCCCCCCTATTCCCCCCGTACCCCCGCTTGTCCCCT
  CCCGACCTTTCCATTTTATTTCCCCTCCCCCCCCCCTCTTCCTTTTCGCCCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_212539
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212539.1, NP_997704.1
• RefSeq Size: 2738 bp
• RefSeq ORF: 2031 bp
• Locus ID: 5580
• UniProt ID: Q05655
• Cytogenetics: 3p21.1
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, GnRH signaling pathway, Neurotrophin signaling pathway, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction
• Gene Summary: The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 3, 6, and 7 all encode the same isoform (c).