CRTAP (NM_006371) Human Untagged Clone

CAT#: SC116156

CRTAP (untagged)-Human cartilage associated protein (CRTAP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRTAP
• Synonyms: CASP; LEPREL3; OI7; P3H5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116156 sequence for NM_006371 edited (data generated by NextGen Sequencing)
  ATGGAGCCGGGGCGCCGGGGGGCCGCGGCGCTGCTAGCGCTGCTGTGCGTGGCCTGCGCG
  CTGCGCGCCGGGCGCGCCCAATACGAACGCTACAGCTTCCGCAGCTTCCCACGGGACGAG
  CTGATGCCGCTCGAGTCGGCCTACCGGCACGCGCTGGACAAGTACAGCGGCGAGCACTGG
  GCCGAGAGCGTGGGCTACCTGGAGATCAGCCTGCGGCTGCACCGCTTGCTGCGCGACAGC
  GAGGCCTTCTGCCACCGCAACTGCAGCGCCGCGCCGCAGCCCGAGCCCGCCGCCGGCCTC
  GCCAGCTATCCCGAGCTGCGCCTCTTCGGGGGCCTGCTGCGCCGCGCGCACTGCCTCAAG
  CGCTGCAAGCAGGGCCTGCCAGCCTTCCGCCAGTCCCAGCCCAGCCGCGAGGTGCTGGCG
  GACTTCCAGCGCCGCGAGCCCTACAAGTTCCTGCAGTTCGCTTACTTCAAGGCAAATAAT
  CTCCCCAAAGCCATCGCCGCTGCTCACACCTTTCTACTGAAGCATCCTGATGACGAAATG
  ATGAAGAGGAACATGGCATATTATAAGAGCCTGCCTGGTGCCGAGGACTACATTAAAGAC
  CTGGAAACCAAGTCATATGAAAGCCTGTTCATCCGAGCAGTGCGGGCATACAACGGTGAG
  AACTGGAGAACATCCATCACAGACATGGAGCTGGCCCTTCCCGACTTCTTCAAAGCCTTT
  TACGAGTGTCTCGCAGCCTGCGAGGGTTCCAGGGAGATCAAGGACTTCAAGGATTTCTAC
  CTTTCCATAGCAGATCATTATGTAGAAGTTCTGGAATGCAAAATACAGTGTGAAGAGAAC
  CTCACCCCAGTTATAGGAGGCTATCCGGTTGAGAAATTTGTGGCTACCATGTATCATTAC
  TTGCAGTTTGCCTATTATAAGTTGAACGACCTGAAGAATGCAGCCCCCTGTGCAGTCAGC
  TATCTGCTCTTTGATCAGAATGACAAGGTCATGCAGCAGAACCTGGTGTATTACCAGTAC
  CACAGGGACACGTGGGGCCTCTCAGATGAGCACTTCCAGCCCAGACCTGAAGCAGTTCAG
  TTCTTTAATGTGACCACACTCCAGAAGGAGCTGTATGACTTTGCTAAGGAAAATATAATG
  GATGATGATGAGGGAGAAGTTGTGGAATATGTGGATGACCTCTTGGAACTGGAGGAGACC
  AGCTAG
  
  Clone variation with respect to NM_006371.4
  1032 t=>g;1044 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006371 unedited
  TCAGAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCTTCCTT
  TCGCCGGGCGCGATGGAGCCGGGGCGCCGGGGGGCCGCGGCGCTGCTAGCGCTGCTGTGC
  GTGGCCTGCGCGCTGCGCGCCGGGCGCGCCCAATACGAACGCTACAGCTTCCGCAGCTTC
  CCACGGGACGAGCTGATGCCGCTCGAGTCGGCCTACCGGCACGCGCTGGACAAGTACAGC
  GGCGAGCACTGGGCCGAGAGCGTGGGCTACCTGGAGATCAGCCTGCGGCTGCACCGCTTG
  CTGCGCGACAGCGAGGCCTTCTGCCACCGCAACTGCAGCGCCGCGCCGCAGCCCGAGCCC
  GCCGCCGGCCTCGCCAGCTATCCCGAGCTGCGCCTCTTCGGGGGCCTGCTGCGCCGCGCG
  CACTGCCTCAAGCGCTGCAAGCAGGGCCTGCCAGCCTTCCGCCAGTCCCAGCCCAGCCGC
  GAGGTGCTGGCGGACTTCCAGCGCCGCGAGCCCTACAAGTTCCTGCAGTTCGCTTACTTC
  AAGGCAAATAATCTCCCCAAAGCCATCGCCGCTGCTCACACCTTTCTACTGAAGCATCCT
  GATGACGAAATGATGAAGAGGAACATGGCATATTATAAGAGCCTGCCTGGTGCCGAGGAC
  TACATTAAAGACCTGGAAACCAAGTCATATGAAAGCCTGTTCATCCGAGCAGTGCGGGCA
  TACAACNGGTGAGAACTGGAGAACATCCATCACAGACAGGGAGCTGGCCCTTCCCGACTT
  CTTCAAGCCTTTTACGAGTGTCTCGCAGCTGGCGAAGGTTCCNAGGGAGATCAAGGACTT
  CAAGGATTTCTACCTTTCCATAGCAGATATTAGGGNNAAAAGTNCTGGAATGCAAATACA
  GTGTGAAGAGAACTCACCCAGTTATAGGAGGCTATCCGGTTGAGAAATTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006371 unedited
  AGAGTCGAGTTTTTTTTTTTTTTTTTTCATCTTCTAGGAATGTTTTTCTTATTTAAAAAA
  TAATACTGATTTTCTGGGAAAAACAAAAAAACAAGCCAGAGAAGACTGCCCTTCAAACCA
  AAATGGTAAGAAAGGCAGCTATGAACATGGGGAAGACAAGTGTGAACATGAGGAAGACAG
  GGATGAAGGTGTGAAAACAGATGTGAGGATAAGAAGACAGGTGTAAAGGTGAAAAAGAGG
  CCGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTGTGGGAGGCCAAGGCAGATGGATC
  ATCTGAAGTCAAGAGTTCGAGACCAGCCTGGCCAACATGGCAAAACCCCGTGTCTACTAA
  AAATACAAAATTACCCGGGCGTGGTGGCACATGCCTGTAATCCCAGGTACTCGGGAGGCT
  GATGCAGCAAAATTGCTTGAACCCAAGAGGCGGAGGTTGCAGTGAGCTGAAAACGTGCCA
  TTGCACTTCAGCCTGTGCAAGAGAGCGAGACTCCATCTCACAAAAAGGTGAAAAAGATAG
  GTGTGAACATGAGGTGGCAGGTGTGAAAATAGGAAACGTCAGCTCACCCCTGATGACTTG
  CTGTTTAAGAAACCGGGGGCTCACTTTTTCCTTTTGGAATTTAAGACAGGTCTTGACGTA
  TCAAACACTCTTGCCTGTTGGAAAAGGACAAAAAACTTGTGCTCTCTTGACCCCAAAAGG
  ATCTTCTTTGCTGCCTGCGGCCAACTGGTCTCTTCCTTCTCAAAGCGTTTCCATTTTCCA
  ACCTCTGCCTATATTCTGCCTTTTTTCGGTTTACCAACCGTACCGCCCTTTCGGATGTGC
  CCCCTTAAAAACTTACTGTTCCAGCTGGCCCGGAAGGCTTTTTATAGCCCCCTTCATCTG
  GGTCCGGAATCATCACTATTTTGTATACCTTTTTTCCCAAAAATCAAAATGTTGGCAGGG
  GGTGTCTCTTGCTTTTCCTTTAAGGCCCGCCTATTGCACCGGCCCCCATTCGTCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006371
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006371.3, NP_006362.1
• RefSeq Size: 6622 bp
• RefSeq ORF: 1206 bp
• Locus ID: 10491
• UniProt ID: O75718
• Cytogenetics: 3p22.3
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass. [provided by RefSeq, Jul 2008]