CRTAP (NM_006371) Human Tagged ORF Clone

CAT#: RC200555

CRTAP (Myc-DDK-tagged)-Human cartilage associated protein (CRTAP)

Product Images

Western blot validation of overexpression lysate (Cat# LY416690) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200555 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CRTAP protein (Cat# TP300555). The protein was produced from HEK293T cells transfected with CRTAP cDNA clone (Cat# RC200555) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRTAP
• Synonyms: CASP; LEPREL3; OI7; P3H5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200555 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGGGCGCCGGGGGGCCGCGGCGCTGCTAGCGCTGCTGTGCGTGGCCTGCGCGCTGCGCGCCG
  GGCGCGCCCAATACGAACGCTACAGCTTCCGCAGCTTCCCACGGGACGAGCTGATGCCGCTCGAGTCGGC
  CTACCGGCACGCGCTGGACAAGTACAGCGGCGAGCACTGGGCCGAGAGCGTGGGCTACCTGGAGATCAGC
  CTGCGGCTGCACCGCTTGCTGCGCGACAGCGAGGCCTTCTGCCACCGCAACTGCAGCGCCGCGCCGCAGC
  CCGAGCCCGCCGCCGGCCTCGCCAGCTATCCCGAGCTGCGCCTCTTCGGGGGCCTGCTGCGCCGCGCGCA
  CTGCCTCAAGCGCTGCAAGCAGGGCCTGCCAGCCTTCCGCCAGTCCCAGCCCAGCCGCGATGTGCTGGCG
  GACTTCCAGCGCCGCGAGCCCTACAAGTTCCTGCAGTTCGCTTACTTCAAGGCAAATAATCTCCCCAAAG
  CCATCGCCGCTGCTCACACCTTTCTACTGAAGCATCCTGATGACGAAATGATGAAGAGGAACATGGCATA
  TTATAAGAGCCTGCCTGGTGCCGAGGACTACATTAAAGACCTGGAAACCAAGTCATATGAAAGCCTGTTC
  ATCCGAGCAGTGCGGGCATACAACGGTGAGAACTGGAGAACATCCATCACAGACATGGAGCTGGCCCTTC
  CCGACTTCTTCAAAGCCTTTTACGAGTGTCTCGCAGCCTGCGAGGGTTCCAGGGAGATCAAGGACTTCAA
  GGATTTCTACCTTTCCATAGCAGATCATTATGTAGAAGTTCTGGAATGCAAAATACAGTGTGAAGAGAAC
  CTCACCCCAGTTATAGGAGGCTATCCGGTTGAGAAATTTGTGGCTACCATGTATCATTACTTGCAGTTTG
  CCTATTATAAGTTGAACGACCTGAAGAATGCAGCCCCCTGTGCAGTCAGCTATCTGCTCTTTGATCAGAA
  TGACAAGGTCATGCAGCAGAACCTGGTGTATTACCAGTACCACAGGGACACGTGGGGCCTCTCAGATGAG
  CACTTCCAGCCCAGACCTGAAGCAGTTCAGTTCTTTAATGTGACCACACTCCAGAAGGAGCTGTATGACT
  TTGCTAAGGAAAATATAATGGATGATGATGAGGGAGAAGTTGTGGAATATGTGGATGACCTCTTGGAACT
  GGAGGAGACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200555 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPGRRGAAALLALLCVACALRAGRAQYERYSFRSFPRDELMPLESAYRHALDKYSGEHWAESVGYLEIS
  LRLHRLLRDSEAFCHRNCSAAPQPEPAAGLASYPELRLFGGLLRRAHCLKRCKQGLPAFRQSQPSRDVLA
  DFQRREPYKFLQFAYFKANNLPKAIAAAHTFLLKHPDDEMMKRNMAYYKSLPGAEDYIKDLETKSYESLF
  IRAVRAYNGENWRTSITDMELALPDFFKAFYECLAACEGSREIKDFKDFYLSIADHYVEVLECKIQCEEN
  LTPVIGGYPVEKFVATMYHYLQFAYYKLNDLKNAAPCAVSYLLFDQNDKVMQQNLVYYQYHRDTWGLSDE
  HFQPRPEAVQFFNVTTLQKELYDFAKENIMDDDEGEVVEYVDDLLELEETS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006371
• ORF Size: 1203 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006371.5
• RefSeq Size: 6668 bp
• RefSeq ORF: 1206 bp
• Locus ID: 10491
• UniProt ID: O75718
• Cytogenetics: 3p22.3
• Protein Families: Secreted Protein, Transmembrane
• MW: 46.5 kDa
• Gene Summary: The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass. [provided by RefSeq, Jul 2008]