UBE2E3 (NM_006357) Human Untagged Clone

CAT#: SC116144

UBE2E3 (untagged)-Human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2E3
• Synonyms: UBCH9; UbcM2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116144 sequence for NM_006357 edited (data generated by NextGen Sequencing)
  ATGTCCAGTGATAGGCAAAGGTCCGATGATGAGAGCCCCAGCACCAGCAGTGGCAGTTCA
  GATGCGGACCAGCGAGACCCAGCCGCTCCAGAGCCTGAAGAACAAGAGGAAAGAAAACCT
  TCTGCCACCCAGCAGAAGAAAAACACCAAACTCTCTAGCAAAACCACTGCTAAGTTATCC
  ACTAGTGCTAAAAGAATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCCTAAT
  TGCAGTGCTGGGCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTTGGTCCA
  CCGGGTTCTGTATATGAAGGTGGTGTGTTTTTTCTGGATATCACATTTTCATCAGATTAT
  CCATTTAAGCCACCAAAGGTTACTTTCCGCACCAGAATCTATCACTGCAACATCAACAGT
  CAGGGAGTCATCTGTCTGGACATCCTTAAAGACAACTGGAGTCCCGCTTTGACTATTTCA
  AAGGTTTTGCTGTCTATTTGTTCCCTTTTGACAGACTGCAACCCTGCGGATCCTCTGGTT
  GGAAGCATAGCCACTCAGTATTTGACCAACAGAGCAGAACACGACAGGATAGCCAGACAG
  TGGACCAAGAGATACGCAACATAA
  
  Clone variation with respect to NM_006357.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006357 unedited
  TCAAATTTTGTAATACGACTTCCTATAGGGGCGGCCGCGATTCGGCACGAGGTAGCGGCG
  CGCGAGCGGGCCGGGCGGGCGGCCGAGTTTTCCAAGAGATAACTTCACCAAGATGTCCAG
  TGATAGGCAAAGGTCCGATGATGAGAGCCCCAGCACCAGCAGTGGCAGTTCAGATGCGGA
  CCAGCGAGACCCAGCCGCTCCAGAGCCTGAAGAACAAGAGGAAAGAAAACCTTCTGCCAC
  CCAGCAGAAGAAAAACACCAAACTCTCTAGCAAAACCACTGCTAAGTTATCCACTAGTGC
  TAAAAGAATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCCTAATTGCAGTGC
  TGGGCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTTGGTCCACCGGGTTC
  TGTATATGAAGGTGGTGTGTTTTTTCTGGATATCACATTTTCATCAGATTATCCATTTAA
  GCCACCAAAGGTTACTTTCCGCACCAGAATCTATCACTGCAACATCAACAGTCAGGGAGT
  CATCTGTCTGGACATCCTTAAAGACAACTGGAGTCCCGCTTTGACTATTTCAAAGGTTTT
  GCTGTCTATTTGTTCCCTTTTGACAGACTGCAACCCTGCGGATCCTCTGGTTGGAAGCAT
  AGCCACTCAGTATTTGACCAACAGAGCAGAACACGACAGGATAGCCAGACAGTGGACCAA
  GAGATACGCAACATAATTCACATAATTTGTATGCAGTGTGAAGGAGCAGAAAGCATCTTC
  TCACTGTGCTGCANATCTTTATAGCCTTTNACATACGGACTTCTGNGGATATGGTATACT
  GGATCTACTCTGGCTTTATCCCTTTGGAGCCTGGGGAGACTTCCAAAAAGGTAAATGCTA
  TNCAGAGTAGAACTTTGTAGCTGTAGATAAGTATAGTTAAAACGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006357 unedited
  CAATCTAGAGTCGAGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTCAGGACCATGTTTTATTGGGTTTGAAAATAATACATAAGGGGCTTTTTGACC
  TACAAAGGGTAAAAGGAAATCCTTTCTTTACAAACTGACAAAGGTAAAAGGTAAAACTTT
  TTTACCAACAGCTTGACCCACATACTCAAAAATAAATCTGGGTTAAAAACAAAGGACATA
  TTTCCTACCCATCTACAGTTACTTTTATAACTTCATACAAATAATGAATTAGGCTAAAAG
  GCCCTATAATATTGGGAAAACTTAAGGACCAGTTTCCTTAGTACATCCCAAAATACTTTT
  TGATATCCCAAAAAACCAAAACTTGCAAGTAGGCGTTTAAAACATAACTAATCTACAGTT
  ACAAAGTTCTACTCTTGATACCATTAACCTTTTTGGGGAGTCTCCCAGGCTCCAAGGGAT
  AAAACCAAAGTAAAATCAGTTTAACCATTTCCCCAAAAGTCCGTATTGAAAAGCCTATAA
  AGATTTGCACCACAGTGAGAAGATCCCTTCTGCTCCTTCACACTGAATACAAATAATGGG
  AATTATGTGGCGTATCTTTTGGCCCACTGTCGGGCTATCCTGCGCGGTCCTGCCCTGTTG
  GAAAAAACCGAGGGGTTATGCTTCCAACCAAAGGATCCCAAGTTTCCATTCTGCCAAAAG
  GAAACAATAAACACAAAACCTTTGAAAAATCAAAGCGGCCCTCCGTGTTTTTAAGATGCC
  ACAAAAAAACCCCTGCATGTGAGGTTCAAGAATAATCTGGGGCGAAAACAACCTTGGCGG
  CTAAAAGGAACTCCGGAAAAGTGGATTCCAAAAAACCCCCCCTTTCATTAACAACCCGGG
  GCACAAAAANTAACCCCCTCAAAAGGTCCCCCCTAGCCCACCCGCCATAAGAGAGAAACA
  GGGTTTCCCTCCCCTTCGAATCTTAACCAAGGAAACAACAGGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006357
• Insert Size: 1350 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006357.2, NP_006348.1
• RefSeq Size: 1559 bp
• RefSeq ORF: 624 bp
• Locus ID: 10477
• UniProt ID: Q969T4
• Cytogenetics: 2q31.3
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3 and 4 encode the same protein.