GADD45G (NM_006705) Human Untagged Clone

CAT#: SC115922

GADD45G (untagged)-Human growth arrest and DNA-damage-inducible, gamma (GADD45G)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GADD45G
• Synonyms: CR6; DDIT2; GADD45gamma; GRP17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115922 sequence for NM_006705 edited (data generated by NextGen Sequencing)
  ATGACTCTGGAAGAAGTCCGCGGCCAGGACACAGTTCCGGAAAGCACAGCCAGGATGCAG
  GGTGCCGGGAAAGCGCTGCATGAGTTGCTGCTGTCGGCGCAGCGTCAGGGCTGCCTCACT
  GCCGGCGTCTACGAGTCAGCCAAAGTCTTGAACGTGGACCCCGACAATGTGACCTTCTGT
  GTGCTGGCTGCGGGTGAGGAGGACGAGGGCGACATCGCGCTGCAGATCCATTTTACGCTG
  ATCCAGGCTTTCTGCTGCGAGAACGACATCGACATAGTGCGCGTGGGCGATGTGCAGCGG
  CTGGCGGCTATCGTGGGCGCCGGCGAGGAGGCGGGTGCGCCGGGCGACCTGCACTGCATC
  CTCATTTCGAACCCCAACGAGGACGCCTGGAAGGATCCCGCCTTGGAGAAGCTCAGCCTG
  TTTTGCGAGGAGAGCCGCAGCGTTAACGACTGGGTGCCCAGCATCACCCTCCCCGAGTGA
  
  Clone variation with respect to NM_006705.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006705 unedited
  CAAACCCAATTCGCACGAGGTGGGCGCGCCGTGCTGAGCTCTGGCTGTCAGTGTGTTCGC
  CCGCGTCCCCTCCGCGCTCTCCGCTTGTGGATAACTAGCTGCTGGTTGATCGCACTATGA
  CTCTGGAAGAAGTCCGCGGCCAGGACACAGTTCCGGAAAGCACAGCCAGGATGCAGGGTG
  CTTGGAAAGCGCTGCATGAGTTGCTGCTGTCGGCGCAGCGTCAGGGCTGCCTCACTGCCG
  GCGTCTACGAGTCAGCCAAAGTCTTGAACGTGGACCCCGACAATGTGACCTTCTGTGTGC
  TGGCTGCGGGTGAGGAGGACGAGGGCGACATCGCGCTGCAGATCCATTTTACGCTGATCC
  AGGCTTTCTGCTGCGAGAACGACATCGACATAGTGCGCGTGGGCGATGTGCAGCGGCTGG
  CGGCTATCGTGGGCGCCGGCGAGGAGGCGGGTGCGCCGGGCGACCTGCACTGCATCCTCA
  TTTCGAACCCCAACGAGGACGCCTGGAAGGATCCCGCCTTGGAGAAGCTCAGCCTGTTTT
  GCGAGGAGAGCCGCAGCGTTAACGACTGGGTGCCCAGCATCACCCTCCCCGAGTGACAGC
  CCGGCGGGGACCTTGGTCTGATCGACGTGGTGACGCCCCGGGGCGCCTAGAGCGCGGCTG
  GCTCTGTGGAGGGGCCCTCCGAGGGTGCCCGAGTGCGGCGTGGAGACTGGCANGCGGNGG
  GGGCGCCTGGAGAGCGAGGAGGCGCGGCCTCCCGAGGAGGGGCCCGGTGGCNGCAGGGCC
  ANGCTGGTCCGAGCTGNAGACTCTGCAAGTGTCTGGAGCGGCTGCTCGCCCAGGAAGGCC
  TAGCTAAGACGTTGGCCCTCAGGCCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006705 unedited
  GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTGTTTCAAAAAGTTTATT
  GTAAAATTCAAAACTTCAACAGCAGCATCCTTTAAAAAACGAAGCATTGCCCGGATCCGT
  TTTGAAAAAGCAGCGCAGTCGGCTAAGTCCTTCACGCTCCTGCAACTGTACCAAGTCCAG
  GGCGCCGCTCCTTCCTGCCGAGCGCAGGCTGCTGAGTCACGCCTGCCCGGCCAGTCTGTC
  CTTCCTGGCCCTGAGGCCAACGTCCTAACCTAGGCCTTCCTGGGCGAGCAGCCGCTCCAG
  ACACTTGCAGAGTCCTCAGCTCGGACCAGCCTGGCCCTGCCGCCACCGGGCCCCTCCTCG
  GGAGGCCGCGCCTCCTCGCTCTCCAGGCGCCCCCCCCGCCTGCCAGTCTCCACGCCGCAC
  TCGGGCACCCTCGGAGGGCCCCTCCACAGAGCCAGCCGCGCTCTAGGCGCCCCGGGGCGT
  CACCACGTCGATCAGACCAAGGTCCCCGCCGGGCTGTCACTCGGGGAGGGTGATGCTGGG
  CACCCAGTCGTTAACGCTGCGGCTCTCCTCGCAAAACAGGCTGAGCTTCTCCAAGGCGGG
  ATCCTTCCAGGCGTCCTCGTTGGGGTTCGAAATGAGGATGCAGTGCAGGTCGCCCGGNGC
  ACCCGCCTTCTCGCCGGNGCCCACGATAGCCGNCAGCCGCTGCACATCGCCCACGCGCAC
  TATGTCGATGTCGNTCTCGCAGCAGAAGCCTGGATCAGCGTAAATGGATCTGCAGCGCGA
  TGTCGNCCTTGTNCTTCTCACCCGCAGNCAGACACAGAAGTCACATTGGCCGGGTCCAGT
  TCAAGACTTTGCTGACTCGTAAACCCCGCAGTGAAGCAACCTGACGCTGGGCGAAGNAGA
  ACTATGCAGNGCTTNCCGGNACCTGATNCTGGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006705
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006705.2, NP_006696.1
• RefSeq Size: 1078 bp
• RefSeq ORF: 480 bp
• Locus ID: 10912
• UniProt ID: O95257
• Cytogenetics: 9q22.2
• Domains: Ribosomal_L7Ae
• Protein Pathways: Cell cycle, MAPK signaling pathway, p53 signaling pathway
• Gene Summary: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]