ADK (NM_006721) Human Untagged Clone

CAT#: SC115890

ADK (untagged)-Human adenosine kinase (ADK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADK
• Synonyms: AK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115890 sequence for NM_006721 edited (data generated by NextGen Sequencing)
  ATGGCAGCTGCTGAGGAGGAGCCGAAGCCCAAAAAGCTGAAGGTGGAGGCGCCGCAAGCG
  CTGAGAGAAAATATTCTCTTTGGAATGGGAAATCCTCTGCTTGACATCTCTGCTGTAGTG
  GACAAAGATTTCCTTGATAAGTATTCTCTGAAACCAAATGACCAAATCTTGGCTGAAGAC
  AAACACAAGGAACTGTTTGATGAACTTGTGAAAAAATTCAAAGTCGAATATCATGCTGGT
  GGCTCTACCCAGAATTCAATTAAAGTGGCTCAGTGGATGATTCAACAGCCACACAAAGCA
  GCAACATTTTTTGGATGCATTGGGATAGATAAATTTGGGGAGATCCTGAAGAGAAAAGCT
  GCTGAAGCCCATGTGGATGCTCATTACTACGAGCAGAATGAGCAGCCAACAGGAACTTGT
  GCTGCATGCATCACTGGTGACAACAGGTCCCTCATAGCTAATCTTGCTGCTGCCAATTGT
  TATAAAAAGGAAAAACATCTTGATCTGGAGAAAAACTGGATGTTGGTAGAAAAAGCAAGA
  GTTTGTTATATAGCAGGCTTTTTTCTTACAGTTTCCCCAGAGTCAGTATTAAAGGTGGCT
  CACCATGCTTCTGAAAACAACAGGATTTTCACTTTGAATCTATCTGCACCGTTTATTAGC
  CAGTTCTACAAGGAATCATTGATGAAAGTTATGCCTTATGTTGATATACTTTTTGGAAAT
  GAGACAGAAGCTGCCACTTTTGCTAGAGAGCAAGGCTTTGAGACTAAAGACATTAAAGAG
  ATAGCCAAAAAGACACAAGCCCTGCCAAAGATGAACTCAAAGAGGCAGCGAATCGTGATC
  TTCACCCAAGGGAGAGATGACACTATAATGGCTACAGAAAGTGAAGTCACTGCTTTTGCT
  GTCTTGGATCAAGACCAGAAAGAAATTATTGATACCAATGGAGCTGGAGATGCATTTGTT
  GGAGGTTTTCTGTCTCAACTGGTCTCTGACAAGCCTCTGACTGAATGTATCCGTGCTGGC
  CACTATGCAGCAAGCATCATAATTAGACGGACTGGCTGCACCTTTCCTGAGAAGCCAGAC
  TTCCACTGA
  
  Clone variation with respect to NM_006721.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006721 unedited
  NAGCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGT
  GGGCTGTAGAGCCAAAGTGGGGTGGGAGCGCGAAGATGGCAGCTGCTGAGGAGGAGCCGA
  AGCCCAAAAAGCTGAAGGTGGAGGCGCCGCAAGCGCTGAGAGAAAATATTCTCTTTGGAA
  TGGGAAATCCTCTGCTTGACATCTCTGCTGTAGTGGACAAAGATTTCCTTGATAAGTATT
  CTCTGAAACCAAATGACCAAATCTTGGCTGAAGACAAACACAAGGAACTGTTTGATGAAC
  TTGTGAAAAAATTCAAAGTCGAATATCATGCTGGTGGCTCTACCCAGAATTCAATTAAAG
  TGGCTCAGTGGATGATTCAACAGCCACACAAAGCAGCAACATTTTTTGGATGCATTGNGA
  TAGATAAATTTGGGGAGATCCTGAAGAGAAAGCTGCTGAAGCCCATGTGGATGCTCATTA
  CTACGAGCAGAATGAGCAGCCAACAGGAACTTGTGCTGCATGCATCACTGGTGAACACAG
  GTCCCTCATAGCTAATCTTGCTGCTGCCAATTGTTATAAAAAGGAAAAACATCTGATCTG
  GAGAAAACTGGATGTTGGTAGAAAAGCAAGAGTTTGTATATAGCAGGCTTTTTTCTACAG
  TTTCCCAGAGTCAGTATTAAAGTGGCTANCATGCTTCTGAAACAACAGGATTTTACTTTG
  ATCTATCTGCACCGTTATTACCCAGTCTACAGGAATCATTGATGAAGTATGCCTTAGTTT
  ATATACTTTTTGGAATGAAACAAAACTGCCCTTTTGCTAAAAGCAAGGCTTGANACTAAA
  ACGTTAAGAGAAGCCAAAAAAACCAACCCTGCGAAAATGACTCGAGAGCCCGAATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006721 unedited
  ACTTGTACCGCGCCGCATTCTAGATCGAGTTTTTTTCTTTTTTTTTTTTTTTTTTGGCAT
  CCTTAAAAAATGATTTATTGGTCTCTGATACTAAGTTATTCTTCAGTGTATTATATTGCT
  GTTCTTCATAATTCCATTAACAAATTGGGTGTCATTTCAGGGCCCAGTATTAAATAATGA
  TCTTTCTTCCTCAGTTTGCTGTACACAGGGTAAAAGACAGAAAAAAATTCCTATATGACA
  AGTTTTTGTTTGTATCCAATTACAGAGTTCCATAAGACTATGATTTGAATATTGTGGTAC
  AACTTAAATTTGGCACATTATTTAATTATCCAAAGTTTATTACATGTTTGAGTGCACATT
  AAAGAGATTATTTGGCATAATTGACAAGATAAAATTTCTGTACCTTATTTGACTATCAGG
  TGTATATGTGGTATATTTATGTATGTGTGTGTATAGACACACATATACTCCTAAATTAAA
  TATATTTGTATAAAAAGCTAAAGATTACTATGTTAAGATTAATTCATTAAAAAATCTATT
  ATATTAAATTGCATCTGCATTCAAAGCAGAAATGTAAAAAAACAAATCAGTGTACTAAAT
  ACACACGAATTTACAAAGTAATTGAAAATGAAATAAAAATTTCTATTGAAATGTTATATT
  CCTTGTTTAAATGGCATTTAAGTGGCACTATCAAACTATGGAAAATAAACATCATTTTTT
  AGATTGTTGAGATAATAAAGATCTACAAGCCTTACATACCCTTGTACCCTCCTAATTAAA
  AATCCGCATTTATATTAGTAGGGAAAAATGGCGAAAATTTTCCTCTTTATAATATGGGGA
  ATCTCAGGAGCCATTAAGGCAATGTCTGCCTTCCGGGGCTTGGGGTTTCAGCCTCTCCAT
  CAAGGAAAGCCTGGCTTTCAGGAAGGTGGCACCCCTCCCCCCTAATTTGAAGCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006721
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006721.2, NP_006712.2
• RefSeq Size: 2018 bp
• RefSeq ORF: 1089 bp
• Locus ID: 132
• UniProt ID: P55263
• Cytogenetics: 10q22.2|10q11-q24
• Domains: pfkB
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (2), alternately referred to as ADK-long, differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b) is longer than isoform a.