UGT1A4 (NM_007120) Human Untagged Clone

CAT#: SC115733

UGT1A4 (untagged)-Human UDP glucuronosyltransferase 1 family, polypeptide A4 (UGT1A4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UGT1A4
• Synonyms: GNT1; hUG-BR1; HUG-BR2; UDPGT; UDPGT 1-4; UGT-1A; UGT-1D; UGT1; UGT1-01; UGT1-04; UGT1.1; UGT1.4; UGT1A; UGT1A1; UGT1A4S; UGT1D
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115733 sequence for NM_007120 edited (data generated by NextGen Sequencing)
  ATGGCCAGAGGACTCCAGGTTCCCCTGCCGCGGCTGGCCACAGGACTGCTGCTCCTCCTC
  AGTGTCCAGCCCTGGGCTGAGAGTGGAAAGGTGTTGGTGGTGCCCACTGATGGCAGCCCC
  TGGCTCAGCATGCGGGAGGCCTTGCGGGAGCTCCATGCCAGAGGCCACCAGGCGGTGGTC
  CTCACCCCAGAGGTGAATATGCACATCAAAGAAGAGAAATTTTTCACCCTGACAGCCTAT
  GCTGTTCCATGGACCCAGAAGGAATTTGATCGCGTTACGCTGGGCTACACTCAAGGGTTC
  TTTGAAACAGAACATCTTCTGAAGAGATATTCTAGAAGTATGGCAATTATGAACAATGTA
  TCTTTGGCCCTTCATAGGTGTTGTGTGGAGCTACTGCATAATGAGGCCCTGATCAGGCAC
  CTGAATGCTACTTCCTTTGATGTGGTTTTAACAGACCCCGTTAACCTCTGCGGGGCGGTG
  CTGGCTAAGTACCTGTCGATTCCTGCTGTGTTTTTTTGGAGGTACATTCCATGTGACTTA
  GACTTTAAGGGCACACAGTGTCCAAATCCTTCCTCCTATATTCCTAAGTTACTAACGACC
  AATTCAGACCACATGACATTCCTGCAAAGGGTCAAGAACATGCTCTACCCTCTGGCCCTG
  TCCTACATTTGCCATACTTTTTCTGCCCCTTATGCAAGTCTTGCCTCTGAGCTTTTTCAG
  AGAGAGGTGTCAGTGGTGGATCTTGTCAGCTATGCATCCGTGTGGCTGTTCCGAGGGGAC
  TTTGTGATGGACTACCCCAGGCCGATCATGCCCAACATGGTCTTCATTGGGGGCATCAAC
  TGTGCCAACGGGAAGCCACTATCTCAGGAATTTGAAGCCTACATTAATGCTTCTGGAGAA
  CATGGAATTGTGGTTTTCTCTTTGGGATCAATGGTCTCAGAAATTCCAGAGAAGAAAGCT
  ATGGCAATTGCTGATGCTTTGGGCAAAATCCCTCAGACAGTCCTGTGGCGGTACACTGGA
  ACCCGACCATCGAATCTTGCGAACAACACGATACTTGTTAAGTGGCTACCCCAAAACGAT
  CTGCTTGGTCACCCGATGACCCGTGCCTTTATCACCCATGCTGGTTCCCATGGTGTTTAT
  GAAAGCATATGCAATGGCGTTCCCATGGTGATGATGCCCTTGTTTGGTGATCAGATGGAC
  AATGCAAAGCGCATGGAGACTAAGGGAGCTGGAGTGACCCTGAATGTTCTGGAAATGACT
  TCTGAAGATTTAGAAAATGCTCTAAAAGCAGTCATCAATGACAAAAGTTACAAGGAGAAC
  ATCATGCGCCTCTCCAGCCTTCACAAGGACCGCCCGGTGGAGCCGCTGGACCTGGCCGTG
  TTCTGGGTGGAGTTTGTGATGAGGCACAAGGGCGCGCCACACCTGCGCCCCGCAGCCCAC
  GACCTCACCTGGTACCAGTACCATTCCTTGGACGTGATTGGTTTCCTCTTGGCCGTCGTG
  CTGACAGTGGCCTTCATCACCTTTAAATGTTGTGCTTATGGCTACCGGAAATGCTTGGGG
  AAAAAAGGGCGAGTTAAGAAAGCCCACAAATCCAAGACCCATTGA
  
  Clone variation with respect to NM_007120.2
  471 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007120 unedited
  GTCGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCTGAGATGGCC
  AGAGGACTCCAGGTTCCCCTGCCGCGGCTGGCCACAGGACTGCTGCTCCTCCTCAGTGTC
  CAGCCCTGGGCTGAGAGTGGAAAGGTGTTGGTGGTGCCCACTGATGGCAGCCCCTGGCTC
  AGCATGCGGGAGGCCTTGCGGGAGCTCCATGCCAGAGGCCACCAGGCGGTGGTCCTCACC
  CCAGAGGTGAATATGCACATCAAAGAAGAGAAATTTTTCACCCTGACAGCCTATGCTGTT
  CCATGGACCCAGAAGGAATTTGATCGCGTTACGCTGGGCTACACTCAAGGGTTCTTTGAA
  ACAGAACATCTTCTGAAGAGATATTCTAGAAGTATGGCAATTATGAACAATGTATCTTTG
  GCCCTTCATAGGTGTTGTGTGGAGCTACTGCATAATGAGGCCCTGATCAGGCACCTGAAT
  GCTACTTCCTTTGATGTGGTTTTAACAGACCCCGTTAACCTCTGCGGNGCGGTGCTGGCT
  AAGTACCTGTCGATTCCTGCTGTGTTTTTTTGGAGGTACATTCCATGTGACTTAGACTTT
  AAGGGCACACAGTGTCCAAATCCTTCCTCCTATATTCCTAAGTTACTAACGACCAATTCA
  GACCACATGACATTCCTGCANAGGGTCAAGAACATGCTCTACCCTCTGGCCCTGTCCTAC
  ATTTGCCATACTTTTTCTGCCCCTTTATGCAGTCTTGCCTCTGAGCTTTTCAGAGAGAGT
  GTCAGTGGTGGATCTTGTCAGCTATGCATCCGTGTGGCTGGTCCGAGGGACTTTGTGATG
  GACTACCCCAGCCGATCATGCCCACATGGTCTTCATGGNGGCATCACTGTGCCACGGGNA
  GCACTATCTCAGATTTGAGCTACTTATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007120 unedited
  NNTTCGACTTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAGA
  ATTATATAAATTTATTAATTTATTTTTAGTTGTAGGAAACATCAGAAAAAAATAAACTTG
  CCCAGCACTTCATAGCTGTATTTTGGGTTTTTATCAAATTCAGCTCCATTTGACATAAGC
  AATGATTATCTTCTCAAATACACCACCCACCAATTTCATAGCATCATTCTTTTTCCCCAA
  AGCAAGAAATCATATGCTGTTCTCAGTGCACTCCAAGCCATTCATTCATTTCACCTACAC
  TCTAAAGGTACAAAGCTTCCCTTCTTTAAACACACAAGGTGGCACCTATGAAGCACGACA
  GAGATGAGGACTGACCATTATTGGTTAAGGATCAATTGCAACCATCTGCAGAAGCCAAAA
  GATAAGATTAAAACTGCCATTTGCAGTAGGGGCAGCCGTGGGACCACCTTTGAATCCCGC
  ACTCCCAAACAGGCCATGTTTCAGAGTAAGAAAAGTAATCTAGAATGCCAGCCTGTCTGC
  ACGTCCTCTGAAAAATGGCACATGTCATCCTGATCAAAGACACCAGAGGGGGCACGATAC
  ATATTCAAATATCTTTACTGACTAGCGAGTCTATTATTTTTATTTAAGAGAATTTTTTAA
  AGCACTCTGGGGCTGATTAATTTATGCAAAGTATTTCCTTAATAAGAATAAAATGAATTT
  AACACTGATTCTGTNTTCAAGTTTGGAAATGACTACGGGAATGGTTCACAATTTTACCTT
  ATTTCCCACCCACTTCTCATGGGGTCTGGNATTTGTGGGCTTTCTTAACTCGCCTTTTGT
  GCCCCAGCATTTCCGGTAGCCATGAGCACACATTTAAAGGTGATGAAGCCACGGTAGCNC
  NAACGCCAAAAGAACCNATCACGTCAGGNATGTACTGGTACCAGTGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007120
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007120.2, NP_009051.1
• RefSeq Size: 2374 bp
• RefSeq ORF: 1605 bp
• Locus ID: 54657
• UniProt ID: P22310
• Cytogenetics: 2q37.1
• Domains: UDPGT
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• Gene Summary: This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. This enzyme has some glucuronidase activity towards bilirubin, although is is more active on amines, steroids, and sapogenins. [provided by RefSeq, Jul 2008]